RESUMO
Objective: To test the cytokine production in HIV infection
Design and methods: Cytokine profiles in two separate studies of HIV patients and controls are presented: Peripheral blood mononuclear cells [PBMC] from patients and controls were tested for the production of [interleukin [IL]-4, IL-5, IL-10, interferon [IFN]-gamma, and TNF-alpha] cytokines by enzyme-linked immunosorbent assay [ELISA]. Both spontaneous and mitogen-induced cytokine production was measured
Results: The serum cytokine profile is altered in HIV patients compared to normal subjects. We examined if HIV binding to resting CD4 T-cells induced production of cytokines, intracellular IL-4, IL-10. TNF-alpha, and IFN-gamma expression 48 hrs after mock- or HIV-exposure of resting T- cells and T-cell. We found that HIV binding to resting T-cells upregulated expression of IFN-gamma and TNF-alpha by 24 hrs], and these remained elevated during 60 hrs of observation HIV binding had no appreciable effect on IL-4 expression and at 48 hrs little effect on IL- 10. EIA measurements of released cytokines in culture supernatants further showed that TNF-alpha and IFN-gamma were produced, but IL-4 was not. Furthermore, by EIA analysis, IL-2 was not induced [data not shown], but IL-5 and IL-10 were at 3 days. It seems likely, then, that HIV-signaled resting CD4 T-cells can provide <
Conclusions: The study herein was performed to more fully characterize HIV-signaled, resting CD4 lymphocytes. We found that HIV signaling also induced production of IFN-gamma, IL-5, IL-10 and TNF-alpha, but not IL-2 or IL-4. These cells, however, did not go into cell cycle. Further, they could secondarily respond to normal proliferation stimuli from anti-CDS cross-linking or PHA and enter into cell cycle and produce a greater number of cytokines, and they did not display any significant activation-induced apoptosis. Thus, resting CD4 lymphocytes are partially activated by HIV binding, resulting in enhanced expression of certain activation markers and cytokines