Computational analysis of phytocompounds with 1,3-β-D-Glucan synthase for antidermatophytic activity.

Skin diseases are the most common infection in humans as well as in animals caused by fungi like yeast, mould and dermatophytes. In this study, in silico analysis of 20 phytocompounds was carried out for their efficacy as antidermatophytic agents using the receptor 1, 3 - β -D-Glucan synthase. The 3D structure of the receptor obtained using Modeller9V8 was validated with Procheck, where Ramachandran plot showed 80.3% of residues in the most favoured region. The phytocompounds and the drugs Echinocandin B and Caspofungin were docked with 1, 3 - β -D-Glucan synthase using Glide. Though all the 20 compounds exhibited lesser energy than both Echinocandin B (-3.3Kcal/mol) and Caspofungin (-1.68 Kcal/mol), Quercetin-3-O-rutinoside exhibited very less energy (-11.56 Kcal/mol). Further, comparing to synthetic drugs, the entire compounds selected for this study showed high interaction with the modeled protein. Hence, the present study concludes that the efficacy of all phytocompounds used in this study act against dermatophyes and which will be very helpful to the researchers working in the area of dermatophye drug developments.

Are Shoulder Surgeons any good at Diagnosing Rotator Cuff tears using Ultrasound ? A Comparative Analysis of Surgeon vs Radiologist

High-resolution ultrasound has gained increasing popularity as an aid in the diagnosis of rotator cuff pathology. With the advent of portable machines; ultrasound has become accessible to clinicians. Aim: This study was conducted to evaluate the accuracy and reliability of ultrasound in diagnosing rotator cuff tears by a shoulder surgeon and comparing their ability to that of a musculoskeletal radiologist. Materials and Methods: Seventy patients undergoing shoulder arthroscopy for rotator cuff pathology underwent preoperative ultrasonography (US). All patients were of similar demographics and pathology. The surgeon used a Sonosite Micromax portable ultrasound machine with a 10-MHz high frequency linear array transducer and the radiologist used a 9-12 MHz linear array probe on a Siemens Antares machine. Arthroscopic diagnosis was the reference standard to which ultrasound findings were compared. Results: The sensitivity in detecting full thickness tears was similar for both the surgeon (92) and the radiologist (94). The radiologist had 100sensitivity in diagnosing partial thickness tears; compared to 85.7for the surgeon. The specificity for the surgeon was 94and 85for the radiologist. Discussion: Our study shows that the surgeons are capable of diagnosing rotator cuff tears with the use of high-resolution portable ultrasound in the outpatient setting. Conclusion: Office ultrasound; by a trained clinician; is a powerful diagnostic tool in diagnosing rotator cuff tears and can be used effectively in running one-stop shoulder clinics