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1.
Chinese Traditional Patent Medicine ; (12): 129-133, 2018.
Artigo em Chinês | WPRIM | ID: wpr-710169

RESUMO

AIM To study the chemical constituents from Perenniporia subacida.METHODS The chloroform-methanol extract from P.subacida was isolated and purified by silica,RP-18 and Sephadex LH-20 column,then the structures of obtained compands were identified by physicochemical properties and spectral data.RESULTS Nine compounds were isolated and identified as 3-hydroxy-4-methoxybenzoicacid (1),2,5-dihydroxybenzoicacid (2),(22E,24R)-ergosta-7,22-dien-3β,5α,6β-triol (3),hydroxylbenzaldehyde (4),4-hydroxyphenyl acetate (5),7-hydroxymethylphthalide (6),(22E,24R)-ergosta-5,7,22-trien-3β-ol (7),(22E,24R)-5 α,8α-epidiory-ergosta-6,22-dien-3β-ol (8),(22E,24R)-ergosta-7,22-dien-3 β,5 β,6β-triol (9).CONCLUSION All the compounds are isolated from Perenniporia subacida for the first time,and compound 6 is a new natural product.

2.
Acta Pharmaceutica Sinica ; (12): 1578-1581, 2014.
Artigo em Inglês | WPRIM | ID: wpr-299094

RESUMO

Two new sesquiterpenoids, named as tyromols A and B (1 and 2), were isolated from cultures of basidiomycete Tyromyces chioneus, along with two previously reported 15-hydroxy-6 α, 12-epoxy-7β, 10αH, 11βH-spiroax-4-ene (3) and agripilol C (4). Compounds 1-4 were separated and purified by silica gel, RP-18, Sephadex LH-20 column chromatography. Their structures were elucidated on the basis of extensive spectroscopic analysis including IR, MS, 1D and 2D NMR experiments.


Assuntos
Basidiomycota , Química , Espectroscopia de Ressonância Magnética , Sesquiterpenos , Química , Sesterterpenos
3.
Chinese Journal of Natural Medicines (English Ed.) ; (6): 685-688, 2014.
Artigo em Inglês | WPRIM | ID: wpr-812215

RESUMO

AIM@#To investigate the chemical constituents of the cultures of Laetiporus sulphureus (Bull.) Murrill.@*METHOD@#Compounds were isolated and purified by various chromatographic techniques. The structure of the new compound was determined by interpretation of MS and 1D-, 2D-NMR spectroscopic data, while the known compounds were identified by comparison of their data with those reported.@*RESULTS@#Three mycophenolic acid derivatives, 6-((2E, 6E)-3, 7-dimethyldeca-2, 6-dienyl)-7-hydroxy-5-methoxy-4-methylphtanlan-1-one (1), 6-((2E, 6E)-3, 7, 11-trimethyldedoca-2, 6, 10-trienyl)-5, 7-dihydroxy-4-methylphtanlan-1-one (2), and 6-((2E, 6E)-3, 7, 11-trimethyldedoca-2, 6, 10-trienyl)-7-hydroxy-5-methoxy-4-methylphtanlan-1-one (3) were isolated.@*CONCLUSION@#Among them, compound 1 was new, and compound 2 exhibited moderate cytotoxicity against HL-60, SMMC-7721, A-549, and MCF-7 cells, with IC50 values of 39.1, 31.1, 27.4, and 35.7 μmol·L(-1), respectively.


Assuntos
Humanos , Agaricales , Produtos Biológicos , Química , Farmacologia , Usos Terapêuticos , Células HL-60 , Células MCF-7 , Estrutura Molecular , Ácido Micofenólico , Química , Neoplasias , Tratamento Farmacológico , Fenóis , Química , Farmacologia , Usos Terapêuticos , Polyporales , Química
4.
Acta Pharmaceutica Sinica ; (12): 446-451, 2012.
Artigo em Chinês | WPRIM | ID: wpr-323021

RESUMO

ATP-binding cassette transporter A1 (ABCA1) promotes cholesterol and phospholipid efflux from cells to lipid-poor apolipoprotein A-I (apoA-I), and plays a key role in the initial steps of the whole process of reverse cholesterol transport (RCT). Upregulation of ABCA1 is beneficial for atherosclerosis (AS) prevention and/or therapy, which indicated that ABCA1 was a target for anti-AS drug development. In the previous study, a high-throughput screening method was established using ABCA1p-LUC HepG2 cell line to find the upregulators of ABCA1. In the present study, compound 2030421B was found using this method, with EC50 of 0.50 microg x mL(-1). The compound was further identified as an upregulator of ABCA1 expression by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting analysis. Studies also showed that the 2030421B could induce apoA-I-mediated cholesterol efflux and inhibit lipids uptake into mouse peritoneal macrophages RAW264.7.


Assuntos
Animais , Humanos , Camundongos , Transportador 1 de Cassete de Ligação de ATP , Transportadores de Cassetes de Ligação de ATP , Genética , Metabolismo , Anticolesterolemiantes , Química , Farmacologia , Apolipoproteína A-I , Metabolismo , Benzaldeídos , Química , Farmacologia , Transporte Biológico , Células Cultivadas , Colesterol , Secreções Corporais , Relação Dose-Resposta a Droga , Células Hep G2 , Ensaios de Triagem em Larga Escala , Metabolismo dos Lipídeos , Lipídeos , Macrófagos Peritoneais , Biologia Celular , Metabolismo , Estrutura Molecular , RNA Mensageiro , Regulação para Cima
5.
China Journal of Chinese Materia Medica ; (24): 1766-1767, 2007.
Artigo em Chinês | WPRIM | ID: wpr-287900

RESUMO

<p><b>OBJECTIVE</b>To investigate the chemical constituents of Boletus vioaceo-fuscus.</p><p><b>METHOD</b>The compounds were isolated with column chromatography. The structures were determined by spectroscopic techniques.</p><p><b>RESULT</b>Six compounds were isolated from the fruiting bodies of Boletus vioaceo-fiuscus. They were identified as ergosta-5, 7, 22-triene-3beta-ol (1), dihydrofuran-2, 5-dione (2), (22E, 24R)-5alpha, 6alpha-epoxyergosta-8, 22-diene-3beta, 7alpha-diol (3), (22E, 24R)-5alpha, 6alpha-epoxyergosta-8 (14), 22-diene-3beta, 7alphadiol (4), cerebroside B (5) and adenosine (6), respectively.</p><p><b>CONCLUSION</b>All the Compounds were obtained from the fruiting bodies of Boletus vioaceo-fiscus for the first time.</p>


Assuntos
Adenosina , Química , Basidiomycota , Química , Cerebrosídeos , Química , Carpóforos , Química , Fitosteróis , Química
6.
Microbiology ; (12)1992.
Artigo em Chinês | WPRIM | ID: wpr-685844

RESUMO

With the constant rise of energy price,it has a great practical meaning of using lignocellulose to produce ethanol.Xylose is a kind of monosaccharide whose content is only less than glucose in most lignocellulosic hydrolysates.There is some difficulty of producing ethanol from lignocellulose by the traditional ethanol production strain Saccharomyces cerevisiae,because it cannot metabolize xylose.People have tried to use genetic engineering technology and cell fusion method to modify Saccharomyces cerevisiae to make it metabolize xylose and produce ethanol for many years.This review indroduced the progress in this field.

7.
Microbiology ; (12)1992.
Artigo em Chinês | WPRIM | ID: wpr-684990

RESUMO

Candida shehatae xyl1 gene and Pichia stipitis xyl2 gene were amplified by PCR and the xyl1 and xyl2 were both placed under the promoter GAL of vector pYES2 to produce the recombinant expression vector pYES2-P12. Subsequently the pYES2-P12 vector was transformed into S. cerevisiae YS58 by LiAc to produce the recombinant yeast YSS8-12. It was indicate that the recombinant yeast YSS8-12 could converse xylose to ethanol with the xylose consumption rate of 81. 3%.

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