RESUMO
We think the strategy of classic natural product-based drug discovery will be an effective way for us to develop new drugs with independent intellectual property. The strategy includes: to study the molecular mechanism of action of classic natural product with chemical genetics and chemical biology approaches firstly; then establish the proper in vitro bioassay or bioassay system based on its molecular mechanism for their pharmacodynamic evaluation; finally, study their structure-activity, structure-toxicity and structure-ADME properties with medicinal chemistry.
Assuntos
Animais , Humanos , Fármacos Anti-HIV , Farmacologia , Antineoplásicos Fitogênicos , Farmacologia , Berberina , Farmacologia , Produtos Biológicos , Química , Farmacologia , Camptotecina , Farmacologia , Descoberta de Drogas , Hipoglicemiantes , Farmacologia , Ácido Oleanólico , Farmacologia , Triterpenos , Farmacologia , Vimblastina , FarmacologiaRESUMO
<p><b>OBJECTIVE</b>To determine actinoside C in the leaves of Actinidia kolomikta with different growth periods.</p><p><b>METHOD</b>The separation was performed at 25 degrees C on ZORBAX Extend C18 column (4.6 mm x 250 mm, 5 microm), using amixture of methanol and water (51:49) as a mobile phase. The flow rate was 1.2 mL x min(-1), and the wavelength for measurement was 267 nm.</p><p><b>RESULT</b>The results showed that the contents of actinoside C in the leaves of A. kolomikta were variety in different growth periods. Actinoside C could reach its highest content in the middle ten days of June, then the content would decrease in the middle ten days of July slightly, it could reach their lowest content in the middle ten days of August.</p><p><b>CONCLUSION</b>The optimal collective date for A. kolomikta are in the middle ten days of June.</p>