RESUMO
PURPOSE: Current studies have demonstrated the neuroprotective effects of dizocilpine (MK-801) in many animal models of brain injury, including hypoxic-ischemic (HI) encephalopathy, trauma and excitotoxicity, but limited data are available for those during the neonatal periods. Here we investigated whether dizocilpine can protect the developing rat brain from HI injury via mediation of nitric oxide synthase. METHODS: In an in vivo model, left carotid artery ligation was done in 7-day-old Sprague-Dawley (SD) rat pups. The animals were divided into three groups; normoxia, hypoxia with operation (HO), and HO treated with dizocilpine at a dose of 10 mg/kg. Hypoxia was made by exposure to a 2 hours period of hypoxic incubator (92% N2, 8% O2). In an in vitro model, embryonic cortical neuronal cell culture of SD rats at 18-day gestation was done. The cultured cells were divided into three groups: normoxia, hypoxia, and hypoxia treated with dizocilpine. The N group was prepared in 5% CO2 incubators and the other groups were placed in 1% O2 incubators (94% N2, 5% CO2) for 16 hours. RESULTS: Dizocilpin treatment significantly reduced the size of brain infarct in the neonatal rat model of HI. Both in the animal and in vitro experiments, expression of iNOS and eNOS were lower in the hypoxia group than in the normoxia group. Meanwhile, the nNOS expression was greater in the hypoxia group. Dizocilpine treatment attenuated these aberrant expressions of NOSs following hypoxic injury. CONCLUSION: Dizocilpine has neuroprotective property over perinatal HI brain injury via mediation of nitric oxide synthase.
Assuntos
Animais , Gravidez , Ratos , Hipóxia , Encéfalo , Lesões Encefálicas , Artérias Carótidas , Técnicas de Cultura de Células , Células Cultivadas , Maleato de Dizocilpina , Incubadoras , Ligadura , Modelos Animais , Negociação , Neurônios , Fármacos Neuroprotetores , Óxido Nítrico , Óxido Nítrico SintaseRESUMO
PURPOSE: Women's weight perception and obesity-related quality of life were analyzed according to BMI (Body Mass Index). METHODS: A survey was conducted using self-report questionnaires from 178 participants. The data analysis included descriptive statistics, frequency, percentage, t-test, ANOVA, Fisher's exact test and Kendall's tau using the SPSS version 14.0. RESULTS: There were significant differences in BMI according to age (F=8.037, p=<.001), weight perception (F=60.71, p<.001), weight control experience (F=2.504, p=.013), weight control method (F=5.839, p=.001) and weight control success (F=-2.451, p=.016). There was a significant difference in obesity-related quality of life according to weight perception in the low weight group (F=5.587, p=.021) and in the obesity group (t=3.419, p=.003). Higher correlations were found between the women's BMI group, weight perception and obesity-related quality of life. CONCLUSION: A program to provide appropriate information for weight perception is needed for the low weight group. There is a need for a program about weight reduction as well as weight perception for the obesity group.