Screening of differentially expressed genes in the jejunum of rats with idiopathic hyperoxaluria / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Idiopathic hyperoxaluria (IH) may be caused by increased endogenous formation or exogenous absorption of oxalic acid. Characterization of the molecular pathogenesis of IH has been hampered by the lack of an ideal animal model. We therefore established a stabile rat IH model in order to analyze variation in gene expression profile in the jejunum and to investigate the association between IH pathogenesis and exogenous absorption of oxalic acid.</p><p><b>METHODS</b>A rat model of IH was established and three female rats with IH were assigned to the study group, while three normal rats served as controls. Total RNA was isolated from the jejunum of rats in the two groups and mRNA was purified, reversely transcribed, labeled with Cy5 or Cy3 and hybridized to 27K Rat Genome Array. Differences in gene expression profile between the 2 groups were analyzed by bioinformatics methods.</p><p><b>RESULTS</b>Comparative analysis revealed that the expression of 517 genes was up-regulated and that of 203 genes was down-regulated by at least two-fold in the jejunum of rats with idiopathic hyperoxaluria. These genes are related to many functions including cell signal transduction, DNA binding and transcription, ATP binding, ion binding and transport, cell receptors, immunity, cyclins, cytoskeleton structure, and metabolic proteins. Kyoto encyclopedia of genes and genomes (KEGG) signaling pathway analysis revealed that the variations of 239 pathway functional changes are statistically significant (P < 0.05).</p><p><b>CONCLUSIONS</b>cDNA microarray can be used effectively to screen differentially expressed genes in the jejunum of rats with idiopathic hyperoxaluria. These differentially expressed genes may underlie idiopathic hyperoxaluria pathophysiology and provide a platform for further studying molecular pathogenetic mechanisms.</p>

Changes of seminal parameters, zinc concentration and antibacterial activity in patients with non-inflammatory chronic prostatitis/chronic pelvic pain syndrome / 中华男科学杂志

<p><b>OBJECTIVE</b>To investigate the seminal parameters, zinc concentration and antibacterial activity in patients with non- inflammatory chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS).</p><p><b>METHODS</b>Seminal parameters, zinc concentration and antibacterial activity of seminal plasma were detected in 60 CP/CPPS patients and 20 normal men.</p><p><b>RESULTS</b>Statistically significant differences were found in the duration of semen liquefaction, sperm vitality, sperm motility, zinc concentration and antibacterial activity of the seminal plasma between the CP/CPPS and the control males ( P < 0.01). Zinc concentration was significantly correlated with the duration of sperm motility (r = 0. 272, P = 0.015) and antibacterial activity of the seminal plasma (r = 0.449, P < 0.01) in the CP/CPPS patients.</p><p><b>CONCLUSION</b>CP/CPPS has a significant negative impact on semen liquefaction, sperm motility and vitality, zinc concentration and antibacterial activity of seminal plasma. The antibacterial activity of seminal plasma is positively correlated with zinc concentration and sperm motility.</p>

Sonic hedgehog and prostate growth regulation / 中华男科学杂志

Sonic hedgehog (Shh) is a key signal regulatory factor in embryonic development. It is reported that Shh signaling plays important roles in prostatic duct differentiation and matrix-epithelium interaction, and thus regulates the development, growth and cell proliferation of the prostate. A disorder in Shh signaling will lead to the production and proliferation of tumor cells. An exploration into the mechanism of Shh signaling in the normal growth and diseased condition of the prostate will offer some significant ideas for the studies on the pathogenesy of prostate diseases.

Facilitation of Shengjingbao to spermatogenesis in oligospermia mouse models and its action mechanisms / 中华男科学杂志

<p><b>OBJECTIVE</b>To investigate the effects of Shengjingbao on spermatogenesis in the mouse model of oligospermia and its action mechanisms.</p><p><b>METHODS</b>Sixty male mice were randomly divided into 4 groups, Shengjingbao (Group 1), Vitamin E (Group 2), blank model control (Group 3) and normal blank control (Group 4). The first three groups were treated by celiac injection of cyclophosphamide for 5 successive days to make models, followed by intragastric administration of Shengjingbao and Vitamin E to Group I and 2, respectively, for 36 days. And then all the mice were sacrificed. The serum testosterone (T) level was determined by radioimmunology, and a suspension was made from the testis and epididymis of one side for sperm analysis, while the testis of the other side was sliced up and stained by HE method and TUNEL technique to detect the count of Leydig cells, the layers of spermatogenic epithelia and the apoptosis of spermatogenic cells.</p><p><b>RESULTS</b>Compared with Group 3, the serum T level, the layers of spermatogenic epithelia and the count of Leydig cells were obviously improved and even exceeded those in Group 2. The positive expression rate of spermatogenic cell apoptosis in Group 1 was evidently lower than Group 2 and 3. The above differences were statistically significant (P < 0.01 or P < 0.05), but no significant difference was noted between Group 1 and 4.</p><p><b>CONCLUSION</b>Shengjingbao can significantly increase the count of Leydig cells, elevate the T level that influences the layers of spermatogenic epithelia, and thus enhance spermatogenesis. The action mechanisms of Shengjingbao may lie in its capacity of inhibiting the apoptosis of spermatogenic cells, increasing the layers of spermatogenic epithelia and facilitating spermatogenesis. T may play a role in inhibiting the apoptosis of spermatogenic cells.</p>

Effects of Bombina maxima protein on growth of bladder cancer cell lines and expession of telomerase / 中国药理学通报

AIM: To study the boosting effect of Bombina maxima protein on bladder cancer cell lines growth and expression of telomerase. METHODS: Cell culture was used to dectect the effects of 3 blad der cancer cell lines growth; TRAP-PCR-ELISA method was used to detect the telomerase express under the ID50 culture concentration. RESULTS: Bombina maxima protein had the strong effect on growth of cell lines; particularly in the first 48 h; no effect was found in telomerase expression. CONCLUSION: Bombina maxima protein has the direct inhibitory effect on bladder cancer cell lines growth; The mechanism of antitumor of Bombina maxima protein is not by telomerase.