Flow cytometric analysis of asialoglycoprotein receptor expression predicts hepatic functional reserve after hepatectomy

To validate a cheaper and more accessible flow cytometry-based method of assessing Asialoglycoprotein Receptor [ASGPR] expression for hepatic functional reserve. A retrospective analysis. Beijing Ditan Hospital, Capital Medical University, Beijing, from January 2011 to October 2013. Patients with Hepatocellular Carcinoma [HCC] undergoing major hepatectomy at Beijing Ditan Hospital, during the study period were retrospectively studied. The fraction of hepatocytes expressing ASGPR in liver tissues was assessed by flow cytometry. Patients were grouped according to the presence or absence of postoperative hepatic dysfunction. The correlation between ASGPR expression and pre-operative liver function parameters with the outcomes of hepatectomy were analyzed. Fewer hepatocytes from patients with postoperative hepatic dysfunction expressed ASGPR [63.3 [57.3 - 68.2]] than from patients without postoperative hepatic dysfunction [72.4 [70.6 - 76.3], p < 0.001]. Multiple logistic regression demonstrated ASGPR levels to be independently correlated with postoperative hepatic dysfunction [Odds ratio 3.34, 95% CI: 2.61-6.02, p < 0.001], and the Receiver Operating Characteristic [ROC] curve for prediction of postoperative liver dysfunction at

All-transretinoic acid regulates Th1/Th2 balance in CD4+ T cells when GATA-3 is deficient / 生物医学与环境科学（英文）

The essential effect of vitamin A on immune function occurs through various mechanisms including direct effect on Th1-Th2 balance modulation. However, it is unclear whether or not vitamin A can regulate Th1-Th2 balance under a strong Th1-polarizing condition. Therefore, the purpose of our study was to examine the effect of vitamin A metabolite all-trans retinoic acid (ATRA) on Th1-Th2 differentiation in CD4+ T cells under GATA-3 deficiency, which can induce Th1-polarizing condition. In the present study, GATA-3 deficiency T cells were induced by siRNA and checked by real-time quantitative PCR and western blot. GATA-3 deficiency CD4+ T cells and normal CD4+ T were treated for 48 h with or without ATRA. The expression of Th1 and Th2 cytokines were detected by qPCR and ELISA. The results would contribute to clarify the knowledge of the role of vitamin A in regulating Th1-Th2 balance under some special conditions, and help to explain the mechanism of immune regulatory function of vitamin A.