Establishment of fingerprint of active fraction from dried body of Catharsius molossus by capillary electrophoresis and analysis by its total quantum statistical moment / 中草药

Objective: To establish capillary electrophoresis (CE) fingerprint of active fraction from the dried body of Catharsius molossus and to explore the feasibility of fingerprint evaluation by total quantum statistical moment method. Methods: Capillary zone electrophoresis (CZE) was used. The electrophoretic conditions were as follows: fused silica capillary column (60 cm × 75 μm), borate solution (75 mmol/L, pH 9.8) as the running buffer, applied voltage of +20 kV, and the detection wavelength of 200 nm. Fingerprint was evaluated by similarity analysis software of Pharmacopeia Committee and total quantum statistical moment method. Results: The CE fingerprint of active fraction from the dried body of C. molossus was established. The similarities in six batches of the active fractions evaluated by similarity analysis software of Pharmacopeia Committee and total quantum statistical moment method had no significant difference. Conclusion: The method is accurate, simple, and reproducible. It could be used for the quality control of active fraction from the dired body of C. molossus. The total quantum statistical moment method could be used to analyze the fingerprint.

Study on acute toxicity of Catharsius molossus / 中草药

Objective: To study the toxicity of Catharsius molossus and the toxic components in C. molossus. Methods: The toxicities of ethanol and water extracts from C. molossus were measured by acute toxicity experiments in mice. The toxic components in the water extract of C. molossus were isolated by organic solvent precipitation and gel permeation chromatography. Results: The mice were ig administered with the ethanol extract (≤ 34.8 g/kg), which indicated that the oral administration was safe. The water extract had the significant acute toxicity with the LD50 value of 19.01 g/kg, and the symptoms were listlessness, abnormal breathing, lack of exercise, and unresponsive to external acoustic stimulation. The purified water extract contained 13% of protein, and the molecular weight distributed from 1.5 × 103 to 3.0 × 104. The protein (molecular weight of 3.0 × 104) may be one of the toxic components of C. molossus. Conclusion: Herbalogical description is consistent with the modern research on the toxicity of C. molossus.

A review on liquid-filled hard gelatin capsules / 中国中药杂志

Novel dosage forms emerges more and more in recent years. One of them is liquid-filled hard gelatin capsules, which adopt gelatin or the hydroxypropyl methyl cellulose (HPMC) as capsule shell. The liquid-filled hard gelatin capsule is increasingly getting attention because of its new-concept dosage form design, which deliver liquid drugs by solid form. The paper mainly introduces application, pharmaceutical manufacturing, quality assessment, prospect of liquid-filled hard gelatin capsules, and focuses on the application and pharmaceutical manufacturing (preparation) of liquid-filled capsule. It is suggested that the capsule is suitable for various liquid or semi-solid natural plant extract and achieve different release profiles. The preparation adopted liquid-filled hard capsules technology. The influence factors concluded property of shell and device of filling. The quality was often evaluated by moisture content of capsule shell, dissolution rate etc. At the same time, it was pointed out that the new dosage form has remarkable marketing prospect and bring profits for enterprises.