Bicyclol alleviates fulminant liver injury induced by TNF-α/D-GalN via inhibiting the mitochondrial apoptosis pathway / 中国药理学通报

Aim To investigate the protective effect of bicyclol on tumor necrosis factor-α/D-galactosamine (TNF-α/D-GalN) induced liver injury and its possible mechanisms. Methods Male C57 mice were given 50, 100, and 200 mg · kg

Donor MHC gene to mitigate rejection of transplantation in recipient mice / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Donor organ rejection continues to be a significant problem for patients receiving transplants. We therefore tested whether transferring a donor's major histocompatibility complex (MHC) gene to the recipient would mitigate the rejection of transplanted hearts in mice.</p><p><b>METHODS</b>H-2K(k) gene from donor mice was amplified using nested polymerase chain reaction (PCR) and ligated into a mammalian expression vector, which was then transfected into thymus ground mass cells collected from the recipients. Clones stably expressing the transgene were then injected into the recipients' thymus visualized using ultrasound. Control mice were administered cells previously transfected with empty vector. Following heart transplantation, cardiac activity was monitored electrocardiographically. Recipient thymus cells were tested for MHC antigenicity using flow cytometry and spleen cells were subjected to mixed lymphocyte culture tests. Finally, the transplanted hearts were sectioned, stained and examined under light microscopy.</p><p><b>RESULTS</b>Southern analysis following nested PCR revealed clear expression of H-2K(k) gene. Following transplantation, electrocardiosignals were detectable highly significantly longer in recipients administered thymal cells expressing donor H-2K(k) than in those receiving control cells. Flow cytometric analysis using an anti-H-2K(k) antibody confirmed its expression in H-2K(k) treated recipients but not in control mice. Mixed lymphocyte cultures containing H-2K(k) treated cells showed significantly less proliferation than those containing control cells. Hearts from control mice showed substantially greater lymphocyte infiltration than those from H-2K(k) treated mice and large areas of necrosis.</p><p><b>CONCLUSION</b>Rejection of transplanted hearts can be mitigated substantially by introducing the donor's MHC into the recipient.</p>

Orthodontic retention and adjustment of the occlusion after orthognathic surgery / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the orthodontic retention and adjustment of the occlusion after orthognathic surgery.</p><p><b>METHODS</b>18 patients were divided into three groups. Group I: Cases with skeletal Class I bimaxillary protrusion treated by subapical osteotomy; Group II: Cases with skeletal Class II mandibular retrusion treated by sagittal split mandibular advancement surgery; Group III: Cases with skeletal Class III treated by Le Fort I osteotomy on maxilla and sagittal split osteotomy on mandible. There were 6 patients in each group. Three kinds of orthodontic elastic tractions were used based on different categories of malocclusion and different types of operation.</p><p><b>RESULTS</b>18 patients attained functional occlusion after the orthodontic occlusion adjustment. There was no relapse and malocclusion by surgery.</p><p><b>CONCLUSION</b>Intermaxillary elastics based on different categories of occlusion and different kinds of surgery can improve the occlusion after orthognathic surgery and attatin the functional occlusion.</p>