RESUMO
AIM:To investigate the effect of proline-spirooxindole on the viability and apoptosis of human nonsmall-cell lung cancer A549 cells.METHODS:The effect of proline-spirooxindole on the viability of A549 cells was determined by CCK-8 assay.The apoptosis was analyzed by flow cytometry.The effects of proline-spirooxindole on the expression of PARP and p53 and the phosphorylation of m TOR were determined by Western blot.RESULTS:After A549 cells were treated with proline-spirooxindole (25, 50 and 100 mg/L) , the cell viability was decreased (P<0.01) compared with DMSO control group.The apoptotic rate was increased compared with DMSO control group (P<0.01).The protein expression of p53 was up-regulated, the increased apoptotic protein cleaved PARP was observed, and the phosphorylation of m TOR was inhibited (P<0.01).CONCLUSION:Proline-spirooxindole inhibits the viability of A549 cells and induces apoptosis, which may be related to the phosphorylation of m TOR.