RESUMO
<p><b>OBJECTIVE</b>To investigate the expression of neural salient serine/arginine-rich protein 1 (NSSR1) in colorectal cancer.</p><p><b>METHODS</b>RT-PCR, Western blot and immunohistochemical staining were used to detect the expression of NSSR1 mRNA and protein in different mouse tissues and human colorectal cancer, respectively.</p><p><b>RESULTS</b>NSSR1 mRNA was expressed in mouse cerebrum, cerebellum, heart, liver, intestine, kidney and lung tissue, but NSSR1 protein was only expressed in neural tissues. In normal human intestinal mucosa, NSSR1 was expressed in the colorectal epithelial cells. In colorectal cancer, NSSR1 was highly expressed in the nucleus of tumor cells.</p><p><b>CONCLUSION</b>The extensive expression of NSSR1 in colorectal cancer cells may hint it's roles in the biological function of colorectal cancer.</p>
Assuntos
Animais , Humanos , Camundongos , Proteínas de Ciclo Celular , Genética , Metabolismo , Colo , Metabolismo , Neoplasias Colorretais , Metabolismo , Proteínas de Neoplasias , Genética , Metabolismo , RNA Mensageiro , Genética , Proteínas de Ligação a RNA , Genética , Metabolismo , Reto , Metabolismo , Proteínas Repressoras , Genética , Metabolismo , Fatores de Processamento de Serina-ArgininaRESUMO
A proposal is presented to improve the original complicated control of the exposure parameters in the direct radiography system (DR). The interface circuitry of the high-voltage generator is redesigned using RS-232 serial COM port. The data and signals are transmitted between the personal computer and the high-voltage generator in the serial mode. A program package is designed to realize the control of the exposure parameters of the high-voltage generator in DR on the PC machine. Experiment results have shown that the proposed console operates steadily and it is capable of providing a more convenient operation console for the direct radiography system.