Study on protective effect of water extract from Sabia parviflora on liver injury in mice induced by acetaminophen / 中国中药杂志

The aim of this study was to observe the protective effect of water extract from Sabia parviflora on mice with acute liver injury induced by acetaminophen, and investigate its possible mechanism. Fifty-eight Kunming mice were divided into 6 groups, 8 in the normal group, 10 in the model group, 10 in the biphenyl diester group, and 10 each in the low, medium and high dose groups. After adaptive feeding for one week, the mice in normal group were intragastrically administered with an equal volume of 0.5% sodium carboxymethylcellulose sodium(CMC-Na), and the mice in other groups were intragastrically administered with corresponding drugs at 20 mL·kg~(-1) once a day. Then acetaminophen(200 mg·kg~(-1)) was administered after the above drug administration except the normal group. The behavior and signs of the experimental animals were observed every day and the samples were taken for experiments on the next day of the final administration. The liver mass and mass index were calculated. The blood was collected from the abdominal aorta and centrifuged to obtain the serum for detecting aspartate aminotransferase(AST) activity and alanine aminotransferase(ALT) activity. The liver tissue homogenate was used to detect superoxide dismutase(SOD) activity, glutathione(glutathione, r-glutamyl cysteingl+glycine, GSH) activity and malondialdehyde(MDA) content. Liver tissue was analyzed for histological analysis. The results showed that S. parviflora could alleviate the lipid peroxidation damage in the liver caused by acetaminophen, reduce the ALT and AST activities in serum, increase the levels of SOD and GSH in liver tissue, decrease the content of MDA in liver tissue, and inhibit the apoptosis. S. parviflora could also improve the live histopathological profile, protect liver cells and restore liver function. Among them, the high dose had the most significant effect and showed dose-effect relationship. This study indicated that S. parviflora had a significant protective effect on acetaminophen-induced liver injury in mice, and its mechanism may be related to its anti-oxidation effect and inhi-bitory effect on apoptosis.

Cardiac surgery ERAS system and practical effects / 医学研究生学报

Objective Enhanced recovery after surgery (ERAS) refers to a series of evidence-based medical evidence that has been used to confirm the effective treatment measures in perioperative period, which contributes to speed up the recovery of patients. We aimed to explore the effect of preoperative ERAS for patients undergoing cardiac surgery. Methods Patients with cardiac surgery of a single-center (third-class hospital in cardiac surgery intensive care unit) were compared 6 months before the implementation of ERAS (n =704) and 6 months after implementation (n = 685), and followed up for 1 month. The primary outcomes were acute renal failure, re-intubation, stroke and 30-day mortality. The secondary outcomes included re-operation rates, bloating, wound infections and lung infections. Results After the implementation of ERAS, the mechanical ventilation time decreased significantly from the median of 6 hours to 4.5 hours (P<0.001). The ICU stay time and hospitalization time after ICU transfer were significantly shorter (P<0.001), and the incidence of adverse events, postoperative abdominal distension and lung infection was significantly lower (P<0.05). Addictionally, the hospitalization cost decreased (P<0.05), and the satisfaction increased (P<0.05). Conclusion ERAS for patients undergoing heart surgery can effectively promote their postoperative recovery, decrease postoperative complications, shorten the length of hospitalization, reduce the cost of hospitalization, then improve patients’ satisfaction. The transformation and application of ERAS in cardiac surgery has important clinical value.