The efficacy of motion control technique based on mobility test in rehabilitation of patients with physiologi-cal sacroiliac joint dysfunction / 实用医学杂志

Objective To explore the short-term efficacy of individualized motion control training for the patients with physiological sacroiliac joint dysfunction. Methods 58 patients were diagnosed as sacroiliac joint dysfunction by standing and sitting stoop test and single leg test. All the patients were randomly divided into two groups. Rotation of the iliac bone was adjusted by muscle energy technique and impact technique in all the patients. The study group received additional exercise control training including local muscle drafting,flexibility and stability. The short-term efficacy was observed. Results VAS and ODI in the two groups were improved significantly after treatment,and there was significant difference between the two groups before treatment(P < 0.01). Three weeks after treatment,the VAS score and ODI in the control group increased,while those in the study group did not change significantly. There was a significant difference in the continuous efficacy of treatment between the two groups(P < 0.05). Conclusions Motion control training based on manipulative exercise test evaluation has a better continuous efficacy for patients with no-specific low back pain and sacroiliac joint dysfunction. It is worth clinically popularizing.

Ultrastructure of periosteal cells induced by bone morphogenetic protein 7 in vitro / 中国组织工程研究

BACKGROUND:Study confirms that bone morphogenetic protein can induce osteogenesis;however the ultrastructure of periosteal cells induced by bone morphogenetic protein-7 remains poorly reported. OBJECTIVE:To study the bioactivity and ultrastructure of periosteal cells induced by bone morphogenetic protein-7 in vitro. METHODS:The primary periosteal cells isolated from adult tibial bone were in vitro cultured, and then divided into experimental group and control group. In the experimental group, cells were cultured with bone morphogenetic protein-7 and culture adjuvant;while cells in the control group were only cultured with the adjuvant. Three samples in each group were tested at 5, 10, 15 days, respectively. The general structure of cultured cells was observed using von Kossa staining, and the ultrastructure was observed under transmission electron microscopy. RESULTS AND CONCLUSION:The periosteal cells in the two groups grew wel in vitro, showing uniform morphology. Early cells were spindle-shaped, with strong three-dimensional sense and ful transparency;mitotic cells were short columnar or cubic shaped, there were a lot of rough endoplasmic reticulum and Golgi complex in osteoblasts under electron microscope. Later stage of cells developed from long fusiform into wide shuttle and irregular shape, there were a large number of matrix vesicles within the cells under the electron microscope. The membrane coating, alkaline phosphatase and calcium-binding protein in the cytoplasm, as wel as calcium crystals were found. The osteogenesis basement and lateral sides appeared projections, which were connected with adjacent bone cells. Induction of bone morphogenetic protein-7 in vitro promotes the osteoblasts proliferation, division and bone formation speed. The results suggest that bone morphogenetic protein-7 can significantly enhance the proliferation ability of osteoblasts in vitro.

Bone morphogenetic protein-7 induces the expression of alkaline phosphatase in periosteal cells / 中国组织工程研究

BACKGROUND:The reports on bone morphogenetic protein-7 as a stimulating factor to induce osteogenic are relatively rare. OBJECTIVE:To study the expression of alkaline phosphatase of periosteal cel s after induced by bone morphogenetic protein-7 in vitro. METHODS:Periosteal cel s were obtained from adult tibial periosteum, and then the periosteal cel s were cultured by routine method in vitro. The cel s were divided into experimental group and control group, and then cultured with bone morphogenetic protein-7 plus osteoblast culture adjuvants and simple osteoblast culture adjuvants, respectively. The phase contrast microscope was used to observe the morphology and ultrastructure of periosteal cel s. Each group was observed at 7, 14 and 21 days, and three samples were observed at each time point. Alkaline phosphatase kit was used to detect the expression of osteoblast-specific markers alkaline phosphatase. RESULTS AND CONCLUSION:After cultured for 7 days, the proliferation of periosteal cel s in the experimental group and the control group was increased obviously, and the expression of alkaline phosphatase was detected but less. The cel s were spindle in shape, while the expression of alkaline phosphatase in the experimental group was higher than that in the control group. After cultured for 14 days, the proliferation of periosteal cel s in the experimental group and the control group was increased obviously, the cel morphology was changed from spindle-shaped to wide spindle-shaped, and the expression of alkaline phosphatase in the experimental group was increased significantly when compared with the control group. After cultured for 21 days, the proliferation of periosteal cel s was detected in the experimental group and the control group, and the proliferation in the experimental group was more significant than that in the control group, the cel morphology was wide spindle-shaped, and the number of alkaline phosphatase in the experimental group was higher than that in the control group. Statistical analysis showed that the positive rate of osteogenic markers alkaline phosphatase of bone morphogenetic protein-7 induced periosteal cel s in the experimental group was higher than that in the control group (Posteogenic and regeneration ability, the bone morphogenetic protein-7 could induce periosteal cel s, promote the expression of alkaline phosphatase, and could induce the periosteal cel s to transform into osteoblasts.

Role of osteogenic factors in bone disease and formation / 中国组织工程研究

BACKGROUND: Many factors are expressed during bone formation and osteonosus, which can induce cartilage to transformed into bone, or induce periosteal cells to transform into chondrocytes.OBJECTIVE: To summarize the osteogenic factors in recent years, and explore the important roles of these factors in bone formation and osteonosus.METHODS: A computer-based online search of Medline database (2008-01/2009-07) and CNKI (2008-01/2009-07) was performed using the key words of "osteogenic factors, bone disease, bone formation, Intercellular Signaling Peptides and Proteins,osteogenesis" in English and "osteogenic factors, bone formation, bone disease, cytokine, stimulating factor" in Chinese. All the articles were limited to the English and Chinese languages. The content about the bone factors, bone formation or osteonosus was included. Repeated or old studies were excluded.RESULTS AND CONCLUSION: According to the exclusion criteria, 158 literatures were collected and the roles of osteogenic factors in bone formation and osteonosus were analyzed. Bone formation and osteonosus are complex processes, influenced by many hormones and systemic or local growth factors. A variety of bone growth factors participate in process of bone healing and bone metabolism-related disease, which act on the target calls through local autocrine/paracrine mode and influence the distant target cells through blood circulation mode. A great number of factors are involved in the process of bone cell proliferation,differentiation, matrix synthesis and osteonosus metabolism, such as the fibroblast growth factor, bone morphogenetic protein,platelet-derived growth factor, transforming growth factor, interleukin-1, insulin-like growth factor, growth hormone etc.Osteogenic factors play an important role in the process of bone formation and bone-related diseases. More and more factors have been used in clinical therapy and bone tissue engineering research, and their effects have been clinically proven.