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Objective:To explore the application of linkage teaching model between hospitals and colleges based on integration of theory and practice in the course of pediatric nursing skills.Methods:Two undergraduate classes of pediatric nursing in a college in 2017 were selected as the experimental group and the control group by random sampling. The experimental group adopted the combined teaching method of colleges and hospitals based on the integration of truth and practice, while the control group adopted the traditional teaching method. The results of skill operation assessment were compared between the two groups, and the results were evaluated by the self-designed teaching effect evaluation questionnaire.Results:The results of the combined nursing skills teaching in colleges and universities were higher in the experimental group (91.24±3.01) than that in the control group (87.33±2.96), the difference was statistically significant ( t value was 5.027, P<0.05). The evaluation score of teaching effect of the experimental group was 62.61 ± 2.94, which was significantly higher than that of the control group (49.67 ± 7.79) ( t value was 7.965, P<0.05). Conclusions:The combined teaching mode of colleges and hospitals based on the integration of theory and practice is beneficial to improve students′ comprehensive quality and learning effect, further shorten the gap between college theory and clinical practice, and is an effective form of pediatric nursing education.
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Objective To explore the long-term effects of dexmedetomidine on the brain development in propofol-induced neonatal rats.Methods Thirty-five seven-day-old Sprague-Dawley rats of both genders,weighing 10-15 g,were randomly divided into seven groups (n =5) using a random number table:normal saline group (group N),intralipid group (group F),propofol 100 mg/kg group (group P),dexmedetomidine 75 μg/kg (group D),dexmedetomidine 25 μg/kg,50 μg/kg and 75μg/kg+propofol 100 mg/kg groups (groups PD25,PD50 and PD75),neonatal rats in each group were treated according to the corresponding dosing regimen.After fully awake,the rats were allowed to mature until postnatal week 9 and the spatial learning and memory capacities were tested by Morris water maze.The rats were sacrificed after the tests.Brain was sliced for determination of hippocampal apoptosis by TUNEL assays and the expression of postsynaptic density protein 95 (PSD95) by immunohistochemistry.Results Compared with group N,the escape latency was significantly prolonged,the times of platform crossing were significantly decreased,the hippocampal apoptosis ratio was significantly increased and the expression of PSD95 was significantly down-regulated in groups P,PD25 and PD50 (P＜0.05).Compared with group P,the escape latency was significantly shortened,the times of platform crossing were significantly increased and the hippocampal apoptosis were significantly decreased in groups PD50 and PD75 (P＜0.05),the expression of PSD95 was up-regulated in group PD75 (P＜0.05).Compared with group PD25,the escape latency was significantly shortened,the number of platform crossing was significantly increased and the hippocampal apoptosis were significantly decreased in groups PD50 and PD75 (P＜0.05),the expression of PSD95 was significantly up-regulated in group PD75 (P＜0.05).Compared with group PD50,the hippocampal apoptosis were significantly decreased,the expression of PSD95 was significantly up-regulated in group PD75 (P＜ 0.05).Conclusion The addition of dexmedetomidine 50,75 μg/kg attenuates propofol-induced neurocognitive impairment in neonatal rats after aducthood,partially by attenuating hippocampal apoptosis and upregulating the expression of PSD95.Dexmedetomidine alone was not neurotoxic to the developing brain.
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Objective To explore the efficacy of quantitative susceptibility mapping (QSM) in the assessment of osteoporosis and the impact factors on the QSM values.Methods A total of 105 volunteers (35 males and 70 females) were recruited in this study.The height,weight,waistline and hipline were measured,and the body mass index was calculated.All the subjects underwent MRI-based QSM and quantitative computed tomography (QCT).The measurement of QSM and QCT values was performed on L3 vertebrae body.According to QCT value,the subjects were divided into three groups (normal,osteopenia and osteoporosis).According to age,the subjects were divided into group I (21-30 years old),group 2 (31-40 years old),group 3 (41-50 years old),group 4 (51-60 years old),and group 5 (＞60 years old).Differences among all groups were compared using one-way ANOVA or Kruskal-Wallis.Results According to QCT value,54 subjects were normal,22 osteopenic and 29 osteoporotic.The QSM value for the subjects with osteoporosis [148.60(109.42,188.81)ppb] was significantly higher than that of normal (P＜0.001)and the osteopenia (P＜0.001).The QSM value for the subjects with osteopenia was significantly higher than the normal (P＜0.001).The coefficient of QSM and BMD was-0.749 (P＜0.001).Multiple linear regression showed age was the independent influence factor for QSM value (r=0.72,P＜0.001),whereas the gender,BMI,waistline and hipline showed no significant difference (P＞0.05).With the increasing of age,the QSM value showed a gradual increasing trend.And there were significant differences of QSM values among the different age groups (P＜0.001).The QSM value of 138.98 (100.37,183.84)ppb for group 5 (＞60 years old) was significantly higher than that of group 1,group 2,and group 3 (P＜0.001).There is no difference between group 5 and group 4 (P＞0.05).The QSM value of 96.62(28.62,143.99)ppb for group 4 (51-60 years old) was significantly higher than that of group 1 and group 2 (P＜0.001).And there was no difference between group 4 and group 3 (P＞0.05).The QSM value of group 1,group 2,and group 3 showed no significant difference (P＞0.05).Conclusions The QSM of bone is feasible in the assessment of osteoporosis and has the potential to be a biomarker providing new insights into osteoporosis.And age is the critical factor affecting QSM value.
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Objective To investigate the correlation between the changes of fat and microcirculation in vertebral marrow and the intervertebral disc degeneration. Methods This was a cross-sectional cohort study. Based on the inclusion and exclusion criteria, all 82 patients were recruited, while 246 lumbar intervertebral disc (L2/3-L4/5) were studied. Each disc was assessed by using Pfirrmann grades. The chemical shift imaging (CSI) was performed to calculate the signal intensity ratio (SIR) of the corresponding upper and under vertebral marrow. And intravoxel incoherent motion (IVIM) imaging was performed to obtain the IVIM parameters of slow apparent diffusion coefficient (Dslow), fast apparent diffusion coefficient (Dfast) and perfusion fraction (f). At the same time, b values of 0, 600 s/mm2 were used to obtain the ADC value of each disc. The ADC values of disc, the SIR values and IVIM parameters of the upper and under vertebral marrow between the different segments and different Pfirrmann grading groups were compared using one-way ANOVA or non-parametric test. The correlation of the Pfirrmann grading and ADC value of disc with the parameters of the vertebral marrow were analyzed, respectively. Results Only the f value of the upper and under vertebral marrow showed significant difference between the different segments groups of L2/3 to L4/5 discs (F=5.351 and 8.482, both P<0.05). The ADC values of discs, the SIR value of the upper vertebral marrow and the Dslow value of the under vertebral marrow had significant difference between the different Pfirrmann grading groups (all P<0.05). The Pfirrmann grading was negatively correlated with the disc ADC values (r=-0.651, P<0.01), and was mildly and positively correlated with the SIR values of the upper and under vertebral marrow (r=0.238 and 0.266, both P<0.01). The disc ADC values had a slightly negative correlation with the SIR value of the upper and under vertebral marrow(r=-0.230 and-0.247, both P<0.01). Conclusions The changes of the SIR value and all IVIM parameters of the vertebral bone marrow were not very obvious with the increasing of the grading of the intervertebral disc degeneration, which may be not an effective supplement for the grading of intervertebral disc degeneration.
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Objective To investigate the correlation between the changes of fat and microcirculation in vertebral marrow and the intervertebral disc degeneration. Methods This was a cross-sectional cohort study. Based on the inclusion and exclusion criteria, all 82 patients were recruited, while 246 lumbar intervertebral disc (L2/3-L4/5) were studied. Each disc was assessed by using Pfirrmann grades. The chemical shift imaging (CSI) was performed to calculate the signal intensity ratio (SIR) of the corresponding upper and under vertebral marrow. And intravoxel incoherent motion (IVIM) imaging was performed to obtain the IVIM parameters of slow apparent diffusion coefficient (Dslow), fast apparent diffusion coefficient (Dfast) and perfusion fraction (f). At the same time, b values of 0, 600 s/mm2 were used to obtain the ADC value of each disc. The ADC values of disc, the SIR values and IVIM parameters of the upper and under vertebral marrow between the different segments and different Pfirrmann grading groups were compared using one-way ANOVA or non-parametric test. The correlation of the Pfirrmann grading and ADC value of disc with the parameters of the vertebral marrow were analyzed, respectively. Results Only the f value of the upper and under vertebral marrow showed significant difference between the different segments groups of L2/3 to L4/5 discs (F=5.351 and 8.482, both P<0.05). The ADC values of discs, the SIR value of the upper vertebral marrow and the Dslow value of the under vertebral marrow had significant difference between the different Pfirrmann grading groups (all P<0.05). The Pfirrmann grading was negatively correlated with the disc ADC values (r=-0.651, P<0.01), and was mildly and positively correlated with the SIR values of the upper and under vertebral marrow (r=0.238 and 0.266, both P<0.01). The disc ADC values had a slightly negative correlation with the SIR value of the upper and under vertebral marrow(r=-0.230 and-0.247, both P<0.01). Conclusions The changes of the SIR value and all IVIM parameters of the vertebral bone marrow were not very obvious with the increasing of the grading of the intervertebral disc degeneration, which may be not an effective supplement for the grading of intervertebral disc degeneration.
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BACKGROUND: In many studies, rats were commonly used as models of retrorsine-induced hepatic injury. Some reports have confirmed that retrorsine cannot inhibit proliferation of mouse hepatic cells. Other reports have shown that retrorsine has inhibitory effects on proliferation of mouse hepatic cells. OBJECTIVE: To study the liver regeneration after hepatic injury by creating mouse models treated with partial hepatectomy combination with retrorsine. METHODS: A total of 40 C57BL/6J mice were equally and randomly assigned to 2 groups. In the partial hepatectomy combined with retrorsine group, intraperitoneal injection of retrorsine 70 mg/kg was conducted, twice, within an interval of 2 weeks. Four weeks later, 2/3 hepatectomy was performed. In the partial hepatectomy group, intraperitoneal injection of saline 70 mg/kg was performed, twice, with an interval of 2 weeks. Four weeks later, 2/3 hepatectomy was performed. At 14 days after partial hepatectomy, the restoration of the livers was observed. The liver cell injury was observed at 3, 7 days with hematoxylin-eosin staining. The hepatocyte proliferation was observed at 3 days with BrdU staining. Oval cell proliferation was observed at 3, 7and 14 days with CK19 and C-kit antibody immunohistochemistry.RESULTS AND CONCLUSION: In the partial hepatectomy group, the damaged liver nearly restored to normal at 14 days after partial hepatectomy, and the result was contrary to partial hepatectomy combined with retrorsine group. Hematoxylin-eosin staining demonstrated that significant degeneration changes in hepatic cells in the partial hepatectomy combined with retrorsine group. BrdU staining showed that hepatocyte proliferation at day 3 was significantly determined in the partial hepatectomy group, but few in the partial hepatectomy combined with retrorsine group. CK19 and C-kit immunohistochemistry demonstrated that visible oval cell proliferation was seen in mice of partial hepatectomy combined with retrorsine group. First of all, hepatic oval cells appeared in portal area and differentiated into hepatic cells and bile duct cells, and then grew into the hepatic lobule gradually. These indicated that retrorsine can obviously inhibit hepatocyte regeneration after liver injury in mice. The model of mice treated with retrorsine and partial hepatectomy could induce oval cell proliferation.