Characteristics of group-occurring lung paragonimiasis in early stage in CT / 中国人兽共患病学报

To investigate the CT appearances in early stage of clustering lung paragonimiasis,9 cases of two clustering lung paragonimiasis caused by eating raw stone-crab and laboratory examination were included in the study.Eight cases consulted by doctors in the hospital and their appearances were retrospectively analyzed.There were pleural effusion of varying degree (n=8) and random distribution sub-pleural pulmonary infiltrative lesions (n=7).The accompany appearances of the latter had lunar halo sign,characteristic tunnel sign (n=1) and peri-bronchitis (n=1).If CT detects pulmonary infiltrative lesions of random distribution within sub-pleura or tunnel sign,combining with the history of eating raw stone crabs and other freshwater fishes,with the rise of eosinophilic granulocytes in peripheral blood,the diagnosis of paragonimiasis should be suggested.

Effects of wild-type (Trp72) and mutant (Arg72) apolipoprotein(a) kringle IV-10 on the proliferation of human arterial smooth muscle cells / 中华医学杂志(英文版)

<p><b>OBJECTIVE</b>To assess the atherogenicity of lipoprotein(a), the effect of the heterogeneity of lysine binding of apolipoprotein(a) [apo(a)], a plasminogen-like glycoprotein component on the proliferation of human arterial smooth muscle cells (SMCs).</p><p><b>METHODS</b>Both wild type (wt) Trp72 and mutant (mut) Trp72-->Arg forms of apo(a) kringle IV-10 were expressed by employing a GST-gene fusion system into E. coli. The proliferation of SMCs was determined by flow cytometry and MTT colorimetry. Enzyme-linked immunosorbent assay (ELISA) assay was used to detect the active form of transforming growth factor beta(1) (TGF-beta(1)).</p><p><b>RESULTS</b>Apo(a) wt-kringle IV-10 that has lysine binding properties possessed a growth-stimulating activity to SMCs on a dose-dependence manner by stimulating cells in the G(1)/G(0) phase of cell cycle to S and G(2)/M phase, and reduced significantly the amounts of endogenous active TGF-beta(1) in culture when compared with the control medium and the GST group (2.4 +/- 0.5 vs 8.6 +/- 1.6 and 9.1 +/- 1.7 ng/ml, P < 0.01). The growth-stimulating effect of apo(a) mut-kringle IV-10 deficient in lysine binding was negligible.</p><p><b>CONCLUSIONS</b>Apo(a) induces SMCs growth by inhibiting the activation of latent TGF-beta(1), an activity that may involve the ability of apo(a) kringle IV-10 to bind lysine. The mitogenic effect of apo(a) wt-kringle IV-10 on SMCs might play an active role in the atherogenic function of lipoprotein(a).</p>

The relationship between LDL oxidation and macrophage myeloperoxidase activity / 中华医学杂志(英文版)

<p><b>OBJECTIVE</b>To explore low density lipoprotein (LDL) oxidation by macrophage myeloperoxidase (MPO) at molecular level.</p><p><b>METHODS</b>Using a mouse macrophage model, we examined the relationship between LDL oxidation and macrophage MPO by measuring macrophage MPO activity, LDL oxidation products, MPO gene expression and cellular orientation of LDL oxidation.</p><p><b>RESULTS</b>MPO gene expression increased to its maximum gradually when the concentration of LDL was increased, and then maintained at that level. NaN(3) inhibied the elevation of MPO activity and LDL oxidation, which was LDL concentration-dependent. After the composition of macrophage membrane was roughly analyzed, it was determined that the contents of MPO and LDL in 5% sucrose were 7.667 and 21 times higher than those in 10% sucrose, respectively.</p><p><b>CONCLUSION</b>LDL is attached to the "microdomain" of the macrophage membrane in which LDL is oxidized by MPO.</p>

Sodium Ferulate protects human aortic smooth muscle cells against oxidized Lipoprotein(a) / 中国病理生理杂志

AIM: To investigate the influences of native and oxidized lipoprotein(a) on human arterial smooth muscle cell (SMC) proliferation, change of intracellular free calcium concentration ([Ca 2+ ] i) and the protective effect of sodium ferulate(SF). METHODS: Lp(a) was oxidized by Cu 2+ and the extent of oxidation was assessed by the MDA content.Human SMC were incubated in culture media with SF for 12 h, then exposed to Lp(a) and oxidized-Lp(a), respectively. MTT colorimetry and flow cytometry were used to evaluated the proliferation of SMC and flurorescent indicator Fura-2/AM was used to determined [Ca 2+ ] i. RESULTS: ox-Lp(a) significantly promoted proliferation of SMC and increased[Ca 2+ ] i compared with Lp(a). SF(40,80 mg/L) remarkedly inhibited SMC proliferation and decreased the rising of [Ca 2+ ] i induced by ox-Lp(a) in a dose-dependent manner, but no effect on SMC proliferation and the increase in [Ca 2+ ] i induced by Lp(a).CONCLUSION: ox-Lp(a) induces the strong growth-promoting effect in SMC through increasing in [Ca 2+ ] i, which might be one of the cellular mechanisms responsible for the higher atherogenic potential of ox-Lp(a) compared with Lp(a), and this process can be prevented by inhibiting of oxidation by SF.

Effect of nitric oxide on transcriptional expression of c-fos and c-jun oncogene of cultured rabbit arterial smooth muscle cells / 中国药学杂志

OBJECTIVE：To study the effcet of Nitric oxide (No) on transcriptional expression of c-fos and c-jun oncogene of cultured rabbit arterial smooth muscle cells (ASMC),and its mechanism.METHODS:(1)To culture rabbit ASMC from explants;(2) To determine if NO,FeSO4,and methylene blue have toxic effect on ASMC by cell counting;(3)RNA isolation from ASMC by Guanidinium Thiocyanate-phenol-chloroform method;(4)RNA-DNA blot hybridization.RESULTS:Under the condition of no toxic effects,NO inhibited the expression of c-fos and c-jun oncogene of ASMC apparently,FeSO4 and methylene blue antagonized the inhibition effcet.CONCLUSION:NO inhibited the expression of c-fos and c-jun oncogene of ASMC through cGMP.This may be related to the important mechanism that NO inhibits the proliferation of ASMC.

Induction of apoptosis in mouse fibroblast cell line L929 by arachidonic acid / 中国病理生理杂志

AIM: To observe whether arachidonic acid (AA) could induce apoptosis in mouse fibroblast cell line L929 and the potential mechanism involved. METHODS: The viability and damaged degree of L929 was monitored by MTT and the release of lactate dehydrogenase (LDH). Lipid peroxidation in L929 was measured as malondialdehyde (MDA) content by colorimetric assay. Hoechst 33258 staining was used to observe AA-induced morphological changes. Agarose gel electrophoresis was used to detect DNA fragmentation. RESULTS: Treatment of L929 cell with AA for 24 h, in the range of 40-160 ?mol/L, caused a great decrease in cell survival and increased MDA contents and the release of LDH simultaneously( P

Study of the relation between low density lipoprotein and macrophage-myeloperoxidase / 中国免疫学杂志

Objective:To study the relation between low density lipoprotein and macrophage myeloperoxidase.Methods:Using the reaction that MPO catalyze the oxidation of o dianisidin hydrochloride,MPO activity was determined.Using reverse transcription polymerase chain reaction(RT PCR),MPO gene expression was determined.The two methods were used to observe the relationship between low density lipoprotein and macrophage myeloperoxidase activity.Results:LDL was able to accelerate the hoist of MPO activity, but its effect is less than LPS OX LDL have no this effect When the time of LDL effect was prolonged, MPO activity was enhancing little by little When the concentration of LDL effect was increased, MPO activity was enhancing to max little by little, then it come to plateau Conclusion:LDL non especially induced the hoist of MPO activity and the swelling of MPO secretion The reason of the hoist of MPO activity was likely to make MPO into action and enhance its secretion, but not MPO gene expression