Palindromic sequence-mediated de novo chromosome translocation in sperm samples derived from normal and oligospermic males / 中华男科学杂志

<p><b>OBJECTIVE</b>To systematically explore the occurrence of a novel type of chromosome translocation in human sperm samples.</p><p><b>METHODS</b>Specific translocation junction fragments were quantified using nested and/or multi-nested PCR in sperm DNA derived from 28 oligospermic patients and 32 normal controls.</p><p><b>RESULTS</b>t(11;22) was detected in 49 samples. At least 4 samples were found to have t(1;22) (p21.2;q11.2), t(17;22) (q11;q11) or t(X;22) (q27;q11). The mutation rate seemed to be associated not with age or semen volume, but with sperm concentration (r = -0.389, P < 0.05) and motility (r = -0.397, P < 0.05). Correlation was not found between homology of palindromic sequences and mutation rate.</p><p><b>CONCLUSION</b>Palindromic sequence mediated chromosome translocation is common in human sperm, and associated with sperm concentration and motility. Measurement of such mutations may provide a molecular-level reference for assessing sperm quality.</p>

Application of VISER in the diagnosis of erectile dysfunction / 中华泌尿外科杂志

Objective To determine the value of vascular indication of sexual excitation response (VISER) in the diagnosis of erectile dysfunction (ED). Methods Fifty three cases of ED underwent PGE1 intracavernous injection,VISER and color duplex ultrasonography.The comparison of the results was performed. Results Forty three cases were in Porst Grade 3, whose average cavernous pressure and carvenous pressure index were 144.29 mmHg and 1.24,respectively.Of them,42 cases were of normal penile vascular function,the remaining one was of venous ED;10 cases were in Porst Grade 2, whose average cavernous pressure and carvenous pressure index were 87.76 mmHg and 0.81,respectively.There were significant differences in the average cavernous pressure and carvenous pressure index between the two groups ( P

Expression of metallothionein and Ki-67 in renal cell carcinoma / 中华泌尿外科杂志

Objective To study the expression of metallothionein(MT),Ki 67 in renal cell carcinoma(RCC) and to evaluate their biological significance. Methods The expression of MT and Ki 67 were assayed by means of immunohistochemical technique in RCC tissues and normal renal tissues.DNA content and S phase fraction(SPF) were measured by flow cytometry(FCM). Results The positive expression rates of MT in RCC tissues and normal renal tissues were 52.5%,78.9% respectively( P