RESUMO
Objective: To develop a method for the determination of berberine hydrochloride (BH) in Zhibai Dihuang Pills. Methods: The BH ion selective electrode was fabricated with a molecularly imprinted polymer (MIP). The process is based on the synthesis of polymers with BH as template, methacrylic acid (MAA) as functional monomer, and ethylene glycol dimethacrylate (EGDMA) as a crosslinker. An ion selective electrode was prepared with this MIP as electric active substance. The content of BH was calculated with working curve. Results: The electromotive force (E) exhibited a good Nernst response to BH concentration over a range of 1-100 μmol/L with a slop of 55.48 mV per decade, and recovery rate was 94.5%-105.1%. Conclusion: The method is rapid, precise, and accurate, and the result is satisfaction.