Policy reconstruction of elderly spouse caregivers from the perspectives of the theory"produc-tive engagement in later life" / 中国卫生政策研究

As one of the core contents of the theory"productive engagement in later life",elderly taking care of family members has created tremendous economic and social values. Family caregiving behavior being widely exis-ted in the Chinese society and stretches for thousands of years, still needs to be interpreted from a completely new theory framework in a contemporary ageing society. As one of the most important theories in Social Gerontology,there is already a consensus reached in western society about the value recognition of family caregiving,but in China's con-text,is still however in the twilight zone. This paper begins with a comprehensive review and analysis of the nega-tive impact of family care on the economic burden and physical and mental health impairments among the elderly caregivers, and then introduces the world-wide development of the"productive engagement in later life"theories and practices which recognize and evaluate the social value of the elderly, a framework for rearranging the social support system for the elderly caregivers and the promotion of the sense of the self-development of the elderly based on gender perspectives, with a view it will also greatly restore the identities and socialization of older peo-ple by putting forward several public policies based on the above theoretical framework and the reduction of the elderly subjectivity and sociality.

Study on construct and expression of synthetic genes encoding spider dragline silk in Escherichia coli / 生物工程学报

Dragline spider silk produced from Nephilia clavipes major ampullate is a natural fibrous protein with specific mechanical properties such as high tensile strength and elasticity. Synthetic gene monomer encoding recombinant spider silk protein, based on the known repetitive protein sequence and partial cDNA sequence of dragline silk, was constructed and expressed. DNA monomer sequences were multimerized to encode high molecular weight synthetic spider silks using a "head-to-tail" construction strategy. Multimer was cloned into pET30a(+), a prokaryotic high potency expression vector, and induced with IPTG. The protein from 8-unit repeat was produced in Escherichia coli at levels up to 20 mg/L. The protein was easily purified with high recovery by using an metal ion affinity chromatography and purity was over 90%. The results of SDS-PAGE and Western blot suggested that the mass of the expression product was about 37 kD. This value and amino acid analysis were consistent with those of theoretic calculation.