Mechanisms and regulation of aluminum-induced secretion of organic acid anions from plant roots / 浙江大学学报（英文版）（B辑：生物医学和生物技术）

Aluminum (Al) is the most abundant metal element in the earth’s crust. On acid soils, at pH 5.5 or lower, part of insoluble Al-containing minerals become solubilized into soil solution, with resultant highly toxic effects on plant growth and development. Nevertheless, some plants have developed Al-tolerance mechanisms that enable them to counteract this Al toxicity. One such well-documented mechanism is the Al-induced secretion of organic acid anions, including citrate, malate, and oxalate, from plant roots. Once secreted, these anions chelate external Al ions, thus protecting the secreting plant from Al toxicity. Genes encoding the citrate and malate transporters responsible for secretion have been identified and characterized, and accumulating evidence indicates that regulation of the expression of these transporter genes is critical for plant Al tolerance. In this review, we outline the recent history of research into plant Al-tolerance mechanisms, with special emphasis on the physiology of Al-induced secretion of organic acid anions from plant roots. In particular, we summarize the identification of genes encoding organic acid transporters and review current understanding of genes regulating organic acid secretion. We also discuss the possible signaling pathways regulating the expression of organic acid transporter genes.

Mechanisms and regulation of aluminum-induced secretion of organic acid anions from plant roots / 浙江大学学报（英文版）（B辑：生物医学和生物技术）

Aluminum (Al) is the most abundant metal element in the earth's crust. On acid soils, at pH 5.5 or lower, part of insoluble Al-containing minerals become solubilized into soil solution, with resultant highly toxic effects on plant growth and development. Nevertheless, some plants have developed Al-tolerance mechanisms that enable them to counteract this Al toxicity. One such well-documented mechanism is the Al-induced secretion of organic acid anions, including citrate, malate, and oxalate, from plant roots. Once secreted, these anions chelate external Al ions, thus protecting the secreting plant from Al toxicity. Genes encoding the citrate and malate transporters responsible for secretion have been identified and characterized, and accumulating evidence indicates that regulation of the expression of these transporter genes is critical for plant Al tolerance. In this review, we outline the recent history of research into plant Al-tolerance mechanisms, with special emphasis on the physiology of Al-induced secretion of organic acid anions from plant roots. In particular, we summarize the identification of genes encoding organic acid transporters and review current understanding of genes regulating organic acid secretion. We also discuss the possible signaling pathways regulating the expression of organic acid transporter genes.

Analysis on residual blood in the pipeline consumables of FENWAL blood cell separator / 医学研究生学报

Objective After the collection of blood donation by plateletpheresis, the residual blood in the pipeline consumables cannot be completely returned to blood donors, resulting in waste. The purpose of this article was to explore a safe and simple operation method in order to reduce the blood loss of donors as much as possible.Methods We randomly selected 10 plasmapheresis donors and adopted single-needle procedure of FENWAL blood cell separator to collect platelets in accordance with the blood cell separator operation manual. The air trap was inverted when the machine was performing suction, the last step of return procedure. The salt water pipeline and the blood return pipeline were disconnected their respective pipeline clip after the return procedure ended and the blood were returned by gravity. Cell counts were performed on the remaining blood in blood return pipeline, air trap and pipeline consumables respectively.Results In pipeline consumables, there was (84.68±4.38)mL residual dilute blood finally, containing equivalent red blood cells in (42.06±4.08)mL whole blood and white blood cells in (214.3±68.09)mL whole blood. In air trap, there was (11.98±3.27)mL blood, containing equivalent red blood cells in (8.32±2.52)mL whole blood and white blood cells in (58.97±24.57)mL whole blood. In blood return pipeline, there was 20 mL blood, containing equivalent red blood cells in (11.18±1.18)mL and white blood cells in (80.74±30.89)mL whole blood.The blood remaining in air trap and blood return pipeline was returned to donors by improved operation method.Conclusion The residual blood in air trap and return pipeline can be returned to donors, which provides technical support for the healthy development of voluntary blood donation.

Prevalence and risk factors for posttraumatic stress disorder among survivors from a coal mining accident after 2 and 10 months / 中南大学学报(医学版)

OBJECTIVE@#To investigate the prevalence of posttraumatic stress disorder (PTSD) among survivors from a coal mining accident after 2 and 10 months and factors related to PTSD.@*METHODS@#To estimate the prevalence of PTSD, 104 miners were surveyed through the PTSD Checklist-Civilian Version (PCL-C). Forty survivors including 24 severe PTSD patients and 24 non-PTSD subjects were evaluated thoroughly.@*RESULTS@#The current prevalence rate among survivors from the coal mining accident at 2 months was 50%. There were significant differences in PTSD symptoms, anxiety and depression, personality, and memory performance between the PTSD patients and non-PTSD controls. State anxiety, time to renew the work, depression, neuroticism, and the place they were staying, and length of service were predictors of PTSD. After 10 months of the accident, 30.6% survivors still met the criterion of PTSD. Compared with 2 months after the accident, PTSD symptoms, anxiety, and memory performance improved clearly, while the depressive symptoms had no significant difference. The state anxiety, time to renew the work, positive coping, emotional balance, and length of service were the factors of PTSD symptom healing.@*CONCLUSION@#The current prevalence of PTSD among survivors from coal mining accident is high. The mining accident has great influence on victims, and psychological or medication interventions are necessary. There are lots of risk factors for the prevalence of PTSD, such as state anxiety, depression, neuroticism, and shorter duration of service. Positive coping may be a beneficial factor for PTSD recovery.