Preparation of the monoclonal antibody against human Bocavirus VP2 protein / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>To express and purify HBoV VP2 protein, and the monoclonal antibody against HBoV VP2 protein was prepared with hybridoma technique.</p><p><b>METHODS</b>The HBoV VP2 cloned into vector pET-30a was expressed in E. coil. After purified by immobilized metal affinity chromatography, the BALB/c mouse was immunized with purified protein as antigen. The positive hybridoma cells were screened with hybridoma technique and ELISA assay. Isotype and titer of the monoclonal antibody were detected.</p><p><b>RESULTS</b>The recombinant HBoV VP2 protein was expressed and purified, and then the monoclonal antibody was obtained with hybridoma technique. The titer of the IgG monoclonal antibody was up to 1:4 x 10(5).</p><p><b>CONCLUSION</b>Monoclonal antibody against recombinant HBoV VP2 protein was prepared and the antibody titer was high. This work may provide a new method in rapid diagnosis and study of HBoV.</p>

Replantation of fingertip amputation in lack of availability of intravenous anastomosis / 中国骨伤

<p><b>OBJECTIVE</b>To discuss the replantation of fingertip amputation in lack of availability of intravenous anastomosis.</p><p><b>METHODS</b>From November 2009 to November 2010, 86 patients (104 fingers) with fingertip amputation were treated with replantatioin, including 64 males and 22 females, with an average age of 26 years ranging from 2 to 64 years. The time from injury to therapy was from 30 min to 12 h, time of broken finger ischemia was from 2.5 to 12 h. Preoperative examination showed no obvious abnormalities. Four different replantation methods were selectively applied to these 104 amputated fingertips of 86 cases: (1) replantation with anastomosis of single or bilateral proper digital artery in 37 fingers; (2) replantation with arteriovenous bypass in 27 fingers; (3) replantation with exclusive anastomosis of digital artery in 24 fingers; (4) replantation with removing the palmar pocket method in 16 fingers.</p><p><b>RESULTS</b>One hundred and two of 104 amputated fingertips were survived. Among these survived fingers,75 cases (92 fingers) were followed-up for 6 to 24 months. According to the assessment standard of Chinese Medical Association of Hand Surgery, the results were excellent in 52 cases, good in 19, poor in 4.</p><p><b>CONCLUSION</b>It benefits to expand the indications and improve the survival rate of replantation of fingertip amputation with the correct choice of different replantation methods according to the injury situation of the broken fingertip artery after debridement under the microscope.</p>

Impact of ageing and related factors on death rate of diabetes mellitus in Beijing / 中华预防医学杂志

<p><b>OBJECTIVE</b>To analyze the ageing and related risk factors affecting the death rate of diabetes mellitus in Beijing, and make a correct assessment of risk factors in changing the death rate of diabetes mellitus.</p><p><b>METHOD</b>The changes of death rate on diabetes mellitus in the past 10 years, from 1991 to 2000 were analyzed and calculated as to making clear what is the effects resulting from the ageing and the risk factors.</p><p><b>RESULTS</b>The death rate of diabetes mellitus was increased to 117.55%, from 1991 to 2000, in which 53.28% were attributing to the ageing and 46.72% to the risk factors.</p><p><b>CONCLUSION</b>The effects of ageing and risk factors on death rate of diabetes mellitus should be different and the ageing should be a factor more important than the other risk factors.</p>