P2Y6 receptor and immunoinflammation / 神经科学通报·英文版

The immunocytes microglia in the central nervous system (CNS) were reported to play a crucial role in neurodegeneration. As a member of P2 receptors family, purinoceptor P2Y6 has attracted much attention recently. Previous studies showed that purinoceptor P2Y6 mainly contributed to microglia activation and their later phagocytosis in CNS, while in immune system, it participated in the secretion of interleukin (IL)-8 from monocytes and macrocytes. So there raises a question: whether purinoceptor P2Y6 also takes part in neuroinflammation? Thus, this review mainly concerns about the properties and roles of purinoceptor P2Y6, including (1) structure of purinoceptor P2Y6; (2) distribution and properties of purinoceptor P2Y6; (3) relationships between purinoceptor P2Y6 and microglia; (4) relationships between purinoceptor P2Y6 and immunoinflammation. Itos proposed that purinoceptor P2Y6 may play a role in neuroinflammation in CNS, although further research is still required.

Rapid Detection of Non-01 Vibrio Cholerae in Haihe River by Vibrio Cholerae Outer Membrane Protein Gene (ompw) PCR Technology / 环境与健康杂志

Objective To detect pollution status of non_01 Vibrio cholerae in Haihe river by Vibrio cholerae outer membrane protein gene (ompw) PCR technology. Methods A pair of Vibrio cholera ompw primers was synthetized to detect non_01 Vibrio cholerae in 102 water samples collected from 5 sampling sites in Haihe river by ompw PCR approach and the results were compared with that of the routine bacterial separation culture method. When amplified by PCR, isolated non_01 Vibrio cholerae strain Vbo35 was used as positive reference, Shigella, E.coli and Salmonella strains were used as negative reference in the study. Results The total positive rate of Vibrio cholerae in 102 water samples was 52% and the amplified products were 588 bp. The detection results of the PCR approach were 100% in accord with that of the routine bacterial separation culture method. Conclusion The results indicated that ompw PCR technology was a highly sensitive and specific method to determination of non_01 Vibrio cholerae in Haihe river water system, it shortened the determination time 4～5 times, and could provided a useful determination method for non_01 Vibrio cholerae.