Treatment of hemifacial atrophy by autologous fat injection / 第二军医大学学报

Objective: To assess the clinical outcome of autologous fat injection in treatment of hemifacial atrophy, so as to search for an ideal treatment of hemifacial atrophy. Methods: Autologous fat granules were obtained by tumescent liposuction. After purification, the fat granules were injected in a multi-strata, multi-tunnel, and multi-spot manner to repair facial introcession abnormality. Patients whose introcession was not improved after the stabilization of the fillings were re-injected with autologous fat granules twice or more until satisfactory outcomes were achieved. Results: All the 7 patients in this study obtained satisfactory outcomes after fat injection and basically reached symmetry. The follow-up result was good after 2 years. Conclusion: Local injection transplantation of autologous fat granules is effective for the treatment of facial depression and hemifacial atrophy, with slight trauma, little complication, and satisfactory outcome.

Reconstruction of distal segment defect of thumb with reverse dorsal radial flap / 中华整形外科杂志

<p><b>OBJECTIVE</b>To introduce the application of reverse dorsal radial flap in reconstruction of distal segment defect of thumb.</p><p><b>METHODS</b>From January 2004 to June 2007, 24 cases of soft tissue defect with exposed bone or tendon at the distal segment of thumb were involved. The flap size ranged from 2 cm x 2 cm to 3 cm x 4 cm.</p><p><b>RESULTS</b>Venous congestion happened in 3 cases with one case of partial necrosis at the distal end of the flap. All the other flaps survived completely. The follow-up period was 6 - 24 months. The appearance and function of the thumbs were satisfactory. The 2-point discrimination was 7 - 11 mm (mean 9.3 mm).</p><p><b>CONCLUSIONS</b>Reverse dorsal radial flap is a good option for reconstruction of distal segment defect of thumb.</p>

Pudendal-thigh flap: anatomic basis and application in repairing and reconstructing male perineal region / 中华外科杂志

<p><b>OBJECTIVE</b>To investigate the anatomic feature of pudendal-thigh flap and to provide anatomic guide for clinical design and application of the flap in repairing and reconstructing male perineal region.</p><p><b>METHODS</b>The clinical anatomy of blood supply and refluence and nerve innervation of the perineal region in 15 male cadavers (30 sides) were investigated. Then reasonable flaps were designed for male patients needing repair and reconstruction of the perineal regions based on the anatomic findings.</p><p><b>RESULTS</b>Perineal region has adequate arterial blood supply, abundant venous refluence and reliable nerve innervation. The area has multi-source blood supplies: external pudendal artery, anterior cutaneous branches of obturator artery, bole and lateral branch of arteriae scrotales posteriores. The locations of the arteries were mainly uniform in the 30 sides. Three vasoganglion composed by these arteries and their branches connecting superior, central and inferior parts of the flap. In the 23 cases, the largest flap was 17 cm x 9 cm, and the smallest was 8 cm x 5 cm. The flaps survived and recovered well, with perfect appearance and sensation. The patients were all satisfied with the operation results.</p><p><b>CONCLUSIONS</b>The pudendal-thigh flap has abundant blood supply. It can be incised conveniently and covers donor sites well. The flap can be used to repair and reconstruct male perineum with ideal appearance and sensation.</p>

Expressions of TGF-betaR I, Smad2, Smad3 and Smad7 in keloids / 中华整形外科杂志

<p><b>OBJECTIVE</b>To investigate the expression of TGF-betaR I, Smad2, Smad3 and Smad7 in keloids, normal scars and normal skins. Discuss the significance of these proteins in the course of keloid.</p><p><b>METHODS</b>Immunohistochemistry method was used to detect the expression intensity and distribution of these proteins in above 3 kinds of different tissues in 44 cases. Statistics was used to analyze the data.</p><p><b>RESULTS</b>The expression of TGF-betaR I were much stronger in keloid than in the other two tissues. The expression of Smad7 were lower in keloids. The increase expression of Smad2,3 were not obvious, but they were found to accumulate in the nucleus.</p><p><b>CONCLUSIONS</b>The results indicate that over-expression of TGF-betaR I, low-expression of Smad7 and accumulation of Smad2,3 may be one of the etiological factors of keloids. This research may provide a new idea to prevent and treat keloids or other fibrosis diseases in the future.</p>

The effects of chitosan on different-derived fibroblasts / 中华整形外科杂志

<p><b>OBJECTIVE</b>To investigate the effects of chitosan on the biological activities of the fibroblasts derived from different tissues.</p><p><b>METHODS</b>The biological activities of the fibroblasts derived from different tissues were evaluated with a MTT method for fibroblast proliferation, photic and electronic microscope for morphologic and subcellular structure, 3H-proline uptake method for collagen secretion and ELISA box for the secretion of TGF-beta 1, FGF-AB, and IL-8.</p><p><b>RESULTS</b>This study showed that the chitosan inhabited the proliferation of the fibroblasts and the secretion of the TGF-beta 1, FGF-AB and collagen of the fibroblasts with a dose-depended manner in the normal skin, hypertrophic scar and keloid groups, but it stimulated the IL-8. However, there were no significant differences among the three groups (P > 0.05).</p><p><b>CONCLUSION</b>The chitosan could inhibit the growth, proliferation, biosynthesis and secretion of the fibroblasts, and it may be used to treat different scars.</p>