Apoptosis of Endothelial Cells Induced by Anti-Platelet Integrin β3 Antibody / 中国实验血液学杂志

OBJECTIVE@#To investigate the damaging of human umbilical vein endothelial cells (HUVEC) induced by antiplatelet integrin β3 antibodies in vitro.@*METHODS@#The serum from 36 chronic ITP patients were collected, flow cytometry and monoclonal antibody specific immobilization of platelet antigen (MAIPA) assay were used to collect antiplatelet integrin β3 antibodies from the serum of the patients. After HUVEC were treated by ITP patient serum (PS) containing anti-integrin β3 antibodies, the cell damage was detected by Lactate dehydrogenase (LDH) assay, cell apoptosis was detected by flow cytometry, the expression of apoptosis-related gene Bax was detected by Reverse transcription-Quantitative real-time PCR (RT-qPCR), and expression of Apoptosis-related signaling pathway protein Akt and related protein Bax were detected by Western blot. HUVEC were treated by PS combined with Akt activator SC79, the cells damage were detected by LDH assay, apoptosis of the cells were detected by flow cytometry, the expression of apoptosis-related gene Bax was detected by RT-qPCR.@*RESULTS@#Among 36 cases of serum from the chronic ITP patients, 5 patients' serum containing anti-integrin β3 antibodies were collected. After HUVEC was treated by PS, the viability of LDH was significant increased（P<0.05）, so as for the apoptosis of the cells（P<0.05）, the expression of gene and protein of Bax was increased up-regulated（P<0.05）, the protein expression of pAkt was down-regulated（P<0.05）. Comparing with HUVEC cultured with PS alone, the viability of LDH of HUVEC treated by PS combined with SC79 was significantly reduced（P<0.05）, so as for the apoptosis of the cells（P<0.05）, and gene expression of Bax was significantly decreased（P<0.05）.@*CONCLUSION@#Anti-integrin β3 serum can cause the damage and apoptosis of HUVEC through Akt signaling pathway，the apoptotic effects of anti-integrin β3 antibodies to HUVEC was effectively reversed by SC79.

Clinical Significance of Minimal Residual Disease Monitoring by Multi-parameter Flow Cytometry before Allogeneic Hematopoietic Stem Cell Transplantation for Prognosis of Acute Leukemia Patients / 中国实验血液学杂志

OBJECTIVE@#To investigate the clinical significance of minimal residual disease (MRD) monitoring by multiparameter flow cytometry (MFC) before allogeneic hematopoietic stem cell transplantation (allo-HSCT) in acute leukemia.@*METHODS@#81 cases of patients with AL treated with allo-HSCT in Department of Hematology, the First Affiliated Hospital of Chongqing Medical University form July 2015 to July 2018 was selected and retorspectively analyed. of which 79 patients were in CR and two patients were in non-CR. The CR group was further divided into two groups of MRD and MRD based on the MRD level prior to HSCT.@*RESULTS@#Among 81 patients, there were statistically significant differences in the three-year overall survival(OS) (CR 82.2%: NCR 0%), cumulative relapse incidence(RI) (CR 17.7%; NCR 100%) and leukemia-free survival rate(LFS) (CR 42.3%: NCR 0%) between CR and NCR group(P<0.05). Among 79 CR patients, MRD was negative in 30 patients while positive in 49 patients, there was significant differences in the three-year overall survival between MRD and MRD group. The results of univariate analysis showed that the MRD group showed lower LFS compared with that of MRD group （10.5% vs 36.2%）(P＜0.001，95%CI).@*CONCLUSION@#MRD patients shows longer LFS as compared with that of MRD patients, therefore, MRD monitoring by MFC before allo-HSCT is very important for the prognosis of the AL patients.

Estabilshment and stemness identification of an asymmetric dividing cell line derived from mouse Lewis lung cancer cells / 基础医学与临床

Objective To establish an asymmetric dividing cell line(LLC-ASD cells) derived from mouse Lewis lung carcinoma cancer cells(LLC-parental cells),and to investigate its stemness features in order to lay a founda-tion for depth studying the function of asymmetric dividing in the cancer biology. Methods In order to obtain asymmetrically dividing LLC cells (LLC-ASD cells) derived from LLC-Parental cells,8 times of consecutive cul-ture,enrichment and collection of floating spheriod forming cells followed by 5 times of consecutive single cell clo-ning were conducted. Immunofluorescence assay was used to visualize and quantify the rate of asymmetric division in LLC-ASD cells labeled by BrdU. For comparing the stem characteristics of LLC-Parental and LLC-ASD, RT-qPCR,clonogenic assay in 6-well plate,single cell spheroid formation assay with agar in 6-well plate and 96-well-plate single cell cloning assay were conducted. In vivo,LLC-parental cells and LLC-ASD cells were subcutaneously transplanted in nude mice to determine the effect of the difference in stem cell like properties on tumorigeneicy. The same lung transplantation into tumor experiment in mice were used to compare the differences in cancer biology. Results Asymmetric dividing cells were found in LLC-ASD cell culture through the BrdU immunofluorescence assay and the rate of asymmetric division in the anaphase cells was as high as 50%。According to the clonogenic assay in 6-well plate,single cell and spheroid formation assay with agar in 6-well plate and 96-well-plate single cell cloning assay in LLC-ASD cells,the results showed that they were more prominent than those in the LLC-Parental cells(P<0.05). In vivo,the tumor metastasis potentials of LLC-ASD was enhanced than that of LLC-parental when transplanted to the C57 mice. Further,the tumorigenic potentials of LLC-ASD cells was also increased.Conclusions The asymmetric dividing cell line derived from mouse Lewis lung carcinoma cancer cell line (LLC-ASD cells) is established which exhibits stemness properties. The establishment and characterization of this model will facilitate the research on the function of asymmetric cell dividing in cancer biology.

Effect of transurethral ureteroscopic holmiumlaser lithotripsy on ureteral calculi / 局解手术学杂志

Objective To explore the clinical effect of the transurethral ureteroscopic holmiumlaser lithotripsy in the treatment of ureteral calculi.Methods This study conducted a retrospective analysis of 205 patients with ureteral calculi from September 2015 to June 2017 in the affiliated hospital of Panzhihua university.According to the surgical method,all the patients were divided into control group (102 cases) who were treated with conventional pneumatic lithotripsy and observation group(103cases) who received transurethral ureteroscopic holmiumlaser lithotripsy.The surgical efficacy,renal function indexes and surgical indexes of the two groups were compared and analyzed respectively.Results The total effective rate of observation group was 97.06％,the control group was 85.00％,the difference between two groups was significantly(P ＜ 0.05).There was no significant difference in Cr and BUN level before surgery between two groups (P ＞ 0.05);after treatment,the Cr and BUN levels of two groups were improved significantly (P ＜ 0.05);while the renal function improved index of observation group was significantly better than that of control group,the difference was significant(P ＜ 0.05).The intraoperative blood loss,operative time and postoperative hospital stay of the observation group were significantly lower than those of control group (P ＜ 0.05).Conclusion The transurethral ureteroscopic holmiumlaser lithotripsy and normal air pressure ballistic were both well treatment for ureteral calculi,but patients with the holmium laser lithotripsy have better renal function indexes and operation index.

Information communication between suicide attempters and their family caregivers and care ability of the family caregivers / 上海交通大学学报（医学版）

Objective·To investigate the information requirements of suicide attempters and their family caregivers, as well as the ability of family caregivers to take care of them. Methods·A total of 148 suspected suicide attempters from Department of Emergency of three hospitals in Shanghai and their family caregivers were enrolled in this study. Each group combined with one patient and one family caregiver. The patients and their family caregivers were interviewed by suicide module of the mini-international neuropsychiatric interview, homemade general information questionnaire, family caregiver task inventory, family adaptability and cohesion scale and information needs willingness questionnaire. Results·There were 84 cases (56.8%) of suicide attempters and 64 cases (43.2%) of non-suicide attempters according to the survey. Linear regression analysis showed that the family's ability of taking care of the patients is positively correlated with family intimacy and negatively correlated with the impact of such illness on the family (P=0.000, P=0.008).The scores of patients' information requirement about the status of disease, the treatment-related information, the way to obtain medical information, the cure probability, precautions after treatment, and self-care ability or support from family caregivers were higher than the expectation scores from their family caregivers. Equally, the scores of information requirement from the patients' family caregivers about above-mentioned information were also higher than the expectation scores from patients. Conclusion·The family care ability is related to the influence of the patients on the life of the family caregivers and family intimacy. For the patients and their family caregivers, there is a great demand for information about disease status and treatment situation, the way of seeking help, care information and so on. More information support and psychological assistance should be provided to suicide attempters and their family caregivers.

The role of 14-3-3σ and TPD52L1 in HaCaT cells treated with low dose ultraviolet B / 中国职业医学

OBJECTIVE: To explore the role of 14-3-3σ and TPD52L1 in apoptosis,proliferation and cell cycle in HaCaT cells treated with low dose ultraviolet B( UVB). METHODS: i) HaCaT cells in logarithmic growth phase were exposed to UVB irradiation( cumulative exposure dose was 2. 97 ×10~(-2)J /cm~2) and were harvested after culture for 6-48 hours.HaCaT cells with no UVB irradiation were set as the control( pseudo-irradiation). The apoptosis of cells was detected by flow cytometry. The cells were divided into the control group and the UVB group exposed to UVB irradiation. They were harvested after being cultured for 0-72 hours,and monotetrazolium assay was used to detect the proliferation ability of cells.ii) HaCaT cells were randomly divided into control group( given pseudo-irradiation) and UVB group. Cells were cultured for 0-24 hours and then harvested. Flow cytometry was used to detect the proportion of G2 / M phase of cells. iii) After HaCaT cells were exposed to low dose of UVB irradiation and cultured for 3-24 or 3-30 hours,they were harvested. In addition,the control was treated with pseudo-irradiation. Real-time fluorescence quantitative polymerase chain reaction was used to detect the mRNA relative expression levels of 14-3-3σ and TPD52L1. The Western blotting was used to detect the protein relative expression of 14-3-3σ and TPD52L1 in cells. RESULTS: i) With 24 hours after UVB irradiation as the cell cycle observation time,the apoptosis of HaCaT cells reached to the maximum value and the proliferation ability to the minimum value( P < 0. 05). ii) After irradiation,the proportion of G2 / M phase in the control group showed a periodic change that it maintained at the highest levels after 6-12 hours of exposure to pseudo-irradiation,and at the time point of 18 hours it was decreased to the lowest value,the proportion of G2 / M phase in the UVB group was higher than that in control group at time points of 6,12 and 18 hours after irradiation( P < 0. 05),and sustained at the highest value,which resulted in significant G2 / M phase block. iii) After UVB treatment,the relative expression of mRNA and protein of 14-3-3σ first increased and then decreased. The relative expression level of mRNA of 14-3-3σ reached the highest level in 3 hours after irradiation( P < 0. 05),and maintained at the peak level at time points of 3-12 hours( P < 0. 05),and then gradually decreased until it returned to normal level 24 hours after irradiation; the relative expression of 14-3-3σ protein began to rise at time point of 6 hours after irradiation( P < 0. 05),and reached the peak value at time point of 18 hours( P < 0. 05),and then gradually decreased,but it did not recover to normal level after irradiation for 30 hours. The relative expression of mRNA of TPD52L1 first decreased and then increased. It reached the lowest level in 12 hours after irradiation( P <0. 05),and then increased gradually and reached the peak value after 24 hours( P < 0. 05). The relative expression of TPD52L1 protein first increased and then decreased. It got to the peak 24 hours after irradiation( P < 0. 05),and 30 hours after irradiation it returned to normal level( P < 0. 05). CONCLUSION: 14-3-3σ and TPD52L1 might jointly play an important role in cell apoptosis,proliferation and G2 / M phase block in HaCaT cells induced by low dose UVB irradiation.

The sexual psychology of human males / 中华男科学杂志

Human sexuality has both its sociological nature and biological nature. Advances in molecular biology has unveiled almost all the biological mysteries of human sexuality. However, many problems in the psychological and sociological aspects have not yet been thoroughly studied. As a matter of fact, these psychological and sociological factors have much influence on sexuality than those biological ones. This paper briefly introduces some factors affecting male sexual psychology, including masturbation and other common sexual psychological dysfunctions.

Antagonistic Mechanisms and Related Properties of Strain H5 Against Black-rot Disease of Dracaena sanderiana / 微生物学通报

A strain no.H5 isolated from Rhizophora stylosa Griff in Zhanjiang had a good antagonistic activity against Aspergillus niger,pathogen of Dracaena Sanderiana black-rot disease.It was identified as Bacillus licheniformis.Dual culture,mycelium growth rate and inhibitory zones were used to test the effect. Strong inhibition was shown against A.niger.Inhibitory ratios of H5 germ-free fermented filtrate on mycelium growth and conidial germination were 91.9%and 100%respectively.In addition,mycelia on the edge of antagonistic band became abnormal and over-branching.Meanwhile,a lot of vesicles appeared on the surface.When treated with heat,acid and alkali,the filtration of H5 was always with stable activity.Precipitate in 55%saturated ammonium sulfate dissolved in phosphate buffer solution maintained most of the activity after high pressure steam sterilization for 25 minutes.It was preliminarily considered as a kind of heat resistant protein.