Quantitative Evaluation Method Trillium tschonoskii / 中国药学杂志

OBJECTIVE: To develop a method for the quantitative evaluation Trillium tschonoskii. METHODS: Selecting three penogenin saponins as indicators, a TLC assay was developed for the identification Trillium tschonoskii, and a RP-HPLC assay was established for the content determination. RESULTS: Three penogenin saponins were clearly distinguished by TLC (CHCl3-MeOH-HCOOH-H2O, 6.5:3.5:0.5:1) and well separated by RP-HPLC (CH3CN-H2O, 42:58). The TLC method showed clear spots, and the RP-HPLC method had good linear relationship within the ranges 0.115-0.690 mg · mL-1 for Paris VIII (1) (r=0.9998), 0.135-0.810 mg · mL-1 for penogenin-3β-O-α-L-rhamnopyranosyl-(1→4)-[-O-α-L-rhamnopyranosyl-(1→2)]-O-β-D-gluco-pyraboside(2) (r=0.9996), and 0.225-1.350 mg · mL-1 for Paris VI (3) (r=0.9999). The average recoveries were 99.3%, 98.3%, and 97.7%, respectively. CONCLUSION: The established method is rapid and sensitive for controlling the quality T. tschonoskii.

Chemical constituents of leaf of Eucommia ulmoides / 中国中药杂志

Ten compounds were isolated from the leaf of Eucommia ulmoides by means of recrystallization and chromatographic techniques such as D-101 macroporous resin, MCI resin, ODS gel, Sephadex LH-20 and Rp-HPLC. Their structures were identified by NMR spectral analyses as kaempferide 3-O-beta-D-glucoside (1), quercetin-3-O-beta-D-glucoside (2), quercetin (3), quercetin-3-O-beta-D-xylosyl-(1-->2)-beta-D-galactoside (4), kaempferol-3-O-alpha-L-rhamnosyl-(1-->6)-beta-D-glucoside (5), (2S,3S)-taxifolin 3-O-beta-D-glucoside (6) ,4-hydroxy cinnamic acid (7), (+)-cycloolivil (8), pinoresinol beta-D-glucoside (9), squalene (10). Among them compounds 1,5-7,10 were isolated from the Eucommia genus for the first time. In the DPPH free radical scavenging assay, compound 2 exhibited significant activity (IC50 13.7 micromol x L(-1)), compared with vitamin C (IC50 59.9 micromol x L(-1)); compounds 1, 3 and 9 showed moderate activity (IC50 161,137, 214 micromol x L(-1)), compared with 2,6-di-tert-butyl-4-methylphenol (IC50 236 micromol x L(-1)); compound 4 and 6 showed weak activity (IC50 264, 299 micromol x L(-1)).

Chemical constituents of leaves of Panax japonicus var. major / 中国中药杂志

Seven compounds were isolated from the leaves of Panax japonicus var. major by chromatographic methods including silica gel, Sephadex LH-20, ODS and semi-preparative HPLC. Their structures were elucidated by their physical and chemical properties and spectral data analysis as 5, 7-dihydroxy-8-methoxyl flavone (1), ginsenoside Rs2 (2), quinquenoside R1 (3), ginsenoside Rs1 (4), notoginsenoside Fe (5), ginsenoside Rd2 (6) and gypenosiden IX (7). Among them, compound 1 was obtained from the Panax genus for the first time, and compounds 2-7 were isolated from this plant for the first time.

Chemical constituents of Jasminum giraldii and their antioxidant activity / 中国中药杂志

Ten compounds were isolated from the barks of Jasminum giraldii by means of various of chromatographic techniques such as silica gel, Sephadex LH-20 and Rp-HPLC. Their structures were identified by spectroscopic data analysis as (+)-medioresinol (1), (+) -syringaresinol (2), syringaresinol-4'-O-beta-D-glucopyranoside (3), oleanic acid (4), 3-methoxy-4-hydroxy-trans-cinnamaldehyde (5), trans-sinapaldehyde (6), syringaldehyde (7), 1-(4-methoxy -phenyl) -ethanol (8), trans-cinnamic acid (9), and 4-(1-methoxyethyl) -phenol (10). Among them, compounds 1-3, 5-8 and 10 were isolated from the J. genus for the first time and compounds 4 and 9 were obtained from J. giraldii for the first time. In the DPPH free radical scavenging assay, compound 1 exhibited significant activity (IC50 55.1 micromol x L(-1)), compared with vitamin C(IC50 59.9 micromol x L(-1)); and compound 2 showed moderate activity (IC50 79.0 micromol x L(-1)), compared with 2, 6-di-tert-butyl4-methylphenol (IC50 236 micromol x L(-1)).