RESUMO
Objective To establish an HPLC method for testing the content of icariin in Yinyangsu capsules.Methods An Lichrospher C18(4.6mm?250mm,5?m) was used as the column,and column C18 as the protecting column.The mobile phase was a mixture of acetonitrile-20g/L acetum(30∶70) in a flow rate of 1.0mL/min.The detected wavelength was 270nm.Column temperature was room temperature and the injection quantity was 10?L.The sample was prepared when we added 50%(V/V) alcohol and then made ultrasonic extraction.Measurement was made after the prepared sample was filtrated on 0.45?m filter paper.Results Icariin in the sample was well separated.The linear range was 10.0-60.0mg/L.The linear equation was C=2.042?10-5A+0.554,while the correlation coefficient was 0.9993;the average recovery was 99.8% and RSD was 0.65%(n=6).Conclusion This method can be used in content measurement of Yinyangsu capsules.
RESUMO
Objective To establish a determination method of ferulic acid in Jianpibuxue tablets.(Methods)HPLC was used with Lichrospher C_(18)(4.6mm?250mm,5?m) as the column.The mobile phase was a mixture of acetonitrile-0.378mol/L acetum(30∶70) and in a flow rate of 0.8mL/min;The detective wavelength was 321nm;Column temperature was room temperature and the injection quantity was 10?L.(Results)Ferulic acid in samples was well separated.The linear range was 1.0-10.0mg/L(r=0.9996).The average recovery was 99.8% and RSD was 0.26 %(n=6).Conclusion This method can be used for quality control of ferulic acid in Jianpibuxue tablets.
RESUMO
Objective To establish a method of high performance liquid chromatogram(HPLC) for measuring matrine and oxymatrine in Zhidaipian Tablets.Methods A Lichrospher-NH2 column(4.6 mm?250 mm,5?m) was used with a mobile phase consisting of acetonitrile-ethanol-0.5 mol/L phosphoric acid(80∶10∶10),and in a flow rate of 1.0 mL/min.The detective wavelength was 212 nm.Column temperature was room temperature and the injection quantity was 20 ?L.Results The samples were well separated.The linear range was the same for the target(1.0-10.0 ?g/mL).The linear equations for matrine was C=1.201?10-4A +0.161,the correlation coefficient was 0.9992,and for oxymatrine was C=1.366?10-4A +0.221,while the correlation coefficient was 0.9996,respectively.The mean recovery rate were 99.9% and 99.4%,respectively.RSD was 1.48% and 4.33%,respectively(n=6).Conclusion The method is simple,reliable,accurate and can be used to the quality control of the Zhidaipian Tablets.