Effect of hyperbaric oxygen therapy on lumbar intertransverse fusion in rabbits / 中南大学学报(医学版)

Objective To observe the effect of hyperbaric oxygen (HBO) therapy on lumbar intertransverse fusion in rabbits. Methods Twenty-eight healthy adult female rabbits underwent lum-bar intertransverse fusion at L5～6 with autogenous iliac bone graft. They were randomly assigned into an HBO group and a control group (n=14). Rabbits were killed 6 weeks after the operation for surgical inspection, X-ray, bone density measurement and histologic analysis. Results The fusion rate of the HBO group evaluated by surgical inspection, and radiologic examination in a blind fashion significantly increased compared with that of the control group (P<0.05). Qualitative histologic as-sessment showed increased bone formation and bone maturity in the HBO group compared with the control group. Conclusion Hyperbaric oxygen therapy could improve the fusion rate of lumbar inter-transverse fusion in rabbits.

Extracorporeal Shock Wave Promotes Postnatal Human Bone Marrow Stromal Cells Osteogenesis In vivo / 生物化学与生物物理进展

It is known that extracorporeal shock wave (SW) may promote healing of fracture. A previous study reported that SW promoted human bone marrow stromal cells (hMSCs) towards osteoblasts in vitro. To study the osteogenesis ability of hMSCs treated by shock wave in porous hydroxyapatite (HA) in vivo, primary hMSCs of SW group and control group were cultured in the porous HA for 2 weeks and then implanted into subcutaneous sites of nude mouse. These implants were harvested and prepared for the biochemical analysis of alkaline phosphatase activity by AKP kit, histological analysis of decalcified and undecalcified sections and morphology by scan electric microscope (SEM), as well as osteocalcin mRNA expression by RT- PCR 4 weeks and 8 weeks after implantation. It showed that cells of SW and control group almost covered the rough surface of HA before implantation and the extracelluar matrix of SW group was abundant by SEM photomicrograph . The histological analysis and SEM photomicrograph showed active bone formation 4 weeks and 8 weeks after implantation, as well as tetracycline labeling under fluoroscopy analysis in SW group. Alkaline phosphatase in supernatants of the implants detected 4 weeks and 8 weeks after implantation in SW group was higher than in control group (P

Surgical treatment of primary tumors of the sacrum / 中国基层医药

Objective To study the clinical characteristics,pathological type,diagnosis,treatment and follow-up result of the primary tumors of the sacrum.Methods The cases of 18 patients with primary tumors of the sacrum were treated by operation(12 cases with chordoma,1 case with osteosarcoma,5 cases with giant cell tumor of bone),patients with chordoma performed by radiotherapy after operation,and all patients had been followed up for 4～8 years.Results 15 patients were cured and 3 patients were recurred after operation.Conclusions Chordoma is the most frequent tumor in primary sacrum tumor.This kind of tumor should be treated by surgical and other assist methods.

Second-look arthroscopy following anterior cruciate ligament reconstruction with a hamstring tendon autograft / 中国微创外科杂志

Objective To arthroscopically re-observe the remodeling and maturity process following anterior cruciate ligament(ACL) reconstruction with a hamstring tendon autograft.Methods Thirty-three patients who had undergone a previous arthroscopic ACL reconstruction with a homolateral hamstring autograft were given a second observation under arthroscope.The interval from initial reconstruction to second-look arthroscopy ranged 2~36 months(mean,11.9 months).According to different intervals,the patients were grouped as 1~,4~,7~,10~,13~,18~,and 25~ months.The shape,color,tension,covering synovial tissue,and vascularity of the grafts were evaluated.Results As the interval increased,the hamstring grafts tended to progress to normal ACL morphologically.Under arthroscopic observation,the tendon grafts were characterized as grayish-white thick ligamentous tissues without synovial membrane and blood vessels after 7 months following initial reconstruction,which corresponded with the maturation period of autogenous grafts and simulated normal ACL.Conclusions Remodeling and maturation of the hamstring autografts progresses with time,which is comparable to patellar tendon autografts.

Clinical research on the arthroscopic hamstring tendon autograft for reconstruction of the anterior cruciate ligament / 中国微创外科杂志

Objective To explore the surgical technique and clinical effect of arthroscopic hamstring tendon autograft on reconstruction anterior cruciate ligament (ACL). Methods 22 patients underwent ACL reconstruction with hamstring tendon autograft under arthroscopy. The mean age was 30 7 years (17years～50 years). Results The motion range of knee joint of all cases was normal or nearly normal. The postoperative Lachman test was ≤1+ in 19 patients, 2+ in 2 patients and 3+ in 1 patient. 20 patients also showed an absent pivot shift. The postoperative Lysholm score was (87 7?9 6)points, and the postoperative score increased significantly compared with the preoperative score ( t =2 33, P

The study of the mechanism of resorpt ion of herniated disc tissue / 中华骨科杂志

0.05).There were significant differences between group B and C pre and postoperatively(P

Arthroscopic reshaping for the treatment of discoid meniscus / 中国微创外科杂志

Objective To explore the therapeutic method and efficacy of discoid meniscus urder arthroscope. Methods 37 patients with 37 discoid menisci underwent arthroscopic lateral partial or total meniscectomy. 33 cases were operated on by reshaping (partial meniscectomy) and 4 cases with extensive laceration in the joint capsule rim of hastring tendon underwent total meniscectomy under arthroscope. Results Bases on Ikeuchi's grading, 19 cases were excellent (51.4%), 13 cases good (35.1%) and 5 cases fair(13.5%). Conclusions Arthroscopic reshaping for discoid meniscus can obtain excellent and good efficacy, so it is recommended that patients with discoid menisci should be treated by reshaping.

Treatment and Rehabilitation of Displaced Proximal Humeral Fracture / 中国医师杂志

Objective To investigate the method and efficacy of treatment of displaced proximal humeral fracture. Methods The clinical data of 43 patients with displaced proximal humeral fracture underwent diagnosis and treatment in the latest 5 years were analysed retrospectively. Among them, twelve cases received conservative treatment and 31 cases were treated by operation. Results All patients had clinical union of humeral fracture during the follow up period of 6 months to 3 years. The necrosis of humeral head happened in 3 cases. The excellent and good rates of shoulder joint function were 80 6% in operation group and 75% in conservative group according to Gongyi Huang's assessment standard. Conclusion The treatment method should be chosen according to types of the humeral fracture and general condition of the patients. The main purpose of the treatment is to regain shoulder joint function.

Histological study on remodeling and maturation of hamstring autograft in postoperative patients / 中华骨科杂志

Objective To probe into the remodeling and maturity process along with extension of time in anterior cruciate ligament (ACL) reconstruction with hamstring autograft in order to confirm the date of maturity postoperatively. Methods Thirty-three patients after arthroscopic ACL reconstruction with hamstring autograft, were undergone second-look arthroscopical surgery. Meantime, biopsy specimens were obtained from the mid-zone of the hamstring graft. As a control group, specimens of normal ACL were obtained from total knee replacement of four cases, and specimens of semitendinous tendons obtained from ACL reconstruction of the four cases. The interval from initial reconstruction to second-look arthroscopy ranged from 2 to 36 months(mean 11.9 months). Patients were divided into different groups by postoperative time such as 1-, 4-, 7-, 10-, 13-, 18- and 25- month group. Thirty-three specimens were ordinarily sliced up into longitudinal sections. The sections were stained with hematoxylin and eosin so that cell and collagen fibrils were observed under light microscope, then compared the results with those of control group. Results Under microscopical observation, it was showed that tissue of hamstring graft was remodeled from more blood vessels and cellular amount to normal one, and from an irregular orientated crimp pattern of collagen to regular orientated crimp pattern of collagen in a time-dependent manner. Decreasing cellular amount, changing cell type, and a regular orientated crimp pattern of collagen of the hamstring graft were observed in 7- month group primarily, comparing with the original ACL. However, there existed a slower process in the other groups that are older than that of 7- month. Conclusion There are well histological features in the process of remodeling and maturation of the hamstring autograft after operation following extension of time, such as better survival of early period, and remodel of vessels and collagen. The process of remodeling and maturation in the hamstring autograft is similar to BPB autograft. The maturation period of the hamstring autograft after ACL reconstruction in human patients appears between 7 month and 9 month postoperatively.

Analysis of CT Pathology and Clinical Manifestation of Lumbar Lateral Recess Stenosis / 中国医师杂志

Objective To explore the normal number of lumbar lateral recess and the relationship between the CT pathology and clinical manifestation in the patients who suffered from lumbar lateral recess stenosis. Methods Lumbar specimen of 15 normal adult corpse and 20 healthy volunteers were observed, and the anterior and posterior(AP) sagittal diameters of normal lumber lateral recess were measured. The CT pathology and clinical manifestation of 174 patients who suffered from lumbar lateral recess stenosis were analysed. Results At the levels of L1 and L2, the lateral recess was found in about 34% normal persons.At the levels of L3 to S1, the lateral recess was found in all healthy persons.All patients who suffered from lumbar lateral recess stenosis had narrow AP sagittal diameters of lumbar lateral recess (

Joint Prosthesis with Artificial Diaphysis for the Treatment of Tumors at the Bone End / 中国医师杂志

Objective To study the operation methods and efficacy of joint prosthesis with artificial diaphysis for the treatment of tumors at the end of bone. Methods 28 patients with tumors at the end of bone had resection and limb salvage by joint prosthesis with artificial diaphysis. These cases consisted of 11 osteosarcomas, 10 bone giant cell tumors, 2 chondrosarcomas, 2 metastatic carcinomas, 1 malignant fibrous histiocytoma,1 aneurysmal bone cyst and 1 osteochondroma. Among them, there were 7 cases of proximal femoral tumors, 6 cases of distal femoral tumors, 10 cases of proximal tibial tumors, 5 cases of proximal humeral tumors. Results The follow-up periods were 16 months to 15 years. Eighteen patients died. Functional activity was satisfactory in 71% of the patients(2 years). Conclusions Joint prosthesis with artificial diaphysis for the treatment of tumors at the end of bone is feasible, and a better system for reattachment of the soft tissues is needed to avoid pain and a persistent limp.

Enhancement of cytotoxicity of Adramycin by neferine in Saos-2 cells and its mechanism / 中国药理学通报

AIM To study the synergistic anticancer effccts of neferine combined with anticancer agents on Saos 2 cell lines and its mechanism. METHODS\ Cytotoxicity assay was tested by MTT method.Cell cycle and apoptosis was detected by PI staining flow cytometty. Expression of Rb protein was examined by indirect immune fluorescent symbol flow cytometty and S P immunohistochemical staining. RESULTS\ 5,10 ?mol?L -1 Nef augmented apoptosis of Saos 2 cells induced by ADM,enhanced the expression of Rb protein in Saos 2 cells and raised the G 1 phase ratio of Saos 2 cells. CONCLUSION Nef can promote chemotherapy sensitivity to ADM in Saos 2 cells. Its mechanism is associatd with the up modulation of Rb protein expression in the Saos 2 cells arrest cells in G 1 phase