Effects of low frequency magnetic stimulation on myelin and inflammation in demyelinated mice / 中华物理医学与康复杂志

Objective:To explore the effect of low frequency magnetic stimulation on myelin and inflammation in the callosum of demyelinated mice.Methods:Thirty-six 6 to 8-week-old male C57BL/6J mice were randomly divided into a control group, a cuprizone (CPZ) group and a magnetic therapy group. The CPZ group and the magnetic therapy group had demyelination induced by feeding a mixed diet containing 0.3% CPZ for 6 weeks, while the control group was given conventional food. The magnetic therapy group was given 50Hz 10mT magnetic stimulation during the 6 weeks for 20min daily, 5 days a week. The body mass of each mouse was observed every 7 days. At the end of the 6th week elevated cross maze experiments were conducted to observe any anxiety state. The myelin sheath in the corpus callosum was observed using Luxol fast blue staining and myelin basic protein (MBP) immunohistochemistry Tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the corpus callosum were detected using enzyme-linked immunosorbent assays.Results:After the 6 weeks of treatment, the average body mass of the mice in the magnetic therapy group had improved significantly compared with the CPZ group. The CPZ group′s times in the elevated cross maze experiments were significantly shorter than those of the control group and also shorter than those of the magnetic therapy group. The Luxol staining showed significant myelin loss in the corpus callosum of the CPZ group, but compared with the CPZ group the average loss of myelin in the magnetic therapy group was significantly less. This was further confirmed by the MBP immunohistochemistry. Compared with the control group, the average expression of MBP in the CPZ group was significantly reduced, while in the magnetic therapy group it was significantly increased. Compared with the control group, the average TNF-α and IL-1β levels in the corpus callosum of the CPZ group increased significantly, but compared with the CPZ group the average levels in the magnetic therapy group had decreased significantly.Conclusions:Low frequency magnetic stimulation improves the body weight and anxiety state of mice. That is probably related to less myelin loss and inhibited inflammatory response in the corpus callosum.

Neurotransmitters after brain injury and their relationship with loss of consciousness / 中华物理医学与康复杂志

Objective:To explore the difference in neurotransmitter levels between persons who had been comatose after a brain injury and healthy counterparts, and to explore the relationship between neurotransmitters and different states of consciousness.Methods:A total of 52 persons with brain injury were analyzed retrospectively and divided into a coma group of 30 with Glasgow coma scale scores ≤9 and a sober group of 22 with scores >9. γ-GABA, 5-hydroxytryptamine, noradrenaline and dopamine levels were measured using encephalofluctuography and compared between the two groups.Results:The levels of all of the neurotransmitters tested were significantly lower in the coma group than in the sober group. There were, however, no significant differences between the two groups in terms of relative levels of the neurorsmters. Logistic regression showed that dopamine deficiency is an important risk factor for coma after a brain injury.Conclusions:Most neurotransmitters decrease significantly among those comatose after a brain injury. Dopamine deficiency seems to be a risk factor for the incidence of coma. Therefore, drugs or rehabilitation therapies which can improve dopamine levels can be considered to help coma patients regain consciousness.

Lower limb muscle mechanics during isokinetic pedaling / 中华物理医学与康复杂志

Objective To document and analyze thepeak force and work during isokinetic pedaling at different linear speeds and the flexion-extension peak force ratio.Methods Thirty healthy adults were tested using the Legpress system of the IsoMED 2000 apparatus.Peak force,work and the flexion-extension peak force ratio were recorded and analyzed during isokinetic pedalingat 5 and 50 cm/s.Results The peak force applied and work done by the lower limbs were both significantly lowerwhen pedaling at 50 cm/s than at 5 cm/s.There was no significant difference in peak force or work between the left and right legs at 50 cm/s.The peak force ratio at 50 cm/s was significantly higher than at 5 cm/s.Conclusion The mechanical characteristics of the leg muscles during isokinetic pedaling vary greatly.It is advisable to choose different linear speeds or training modes accordingly.

Efficacy of Process Management on Bladder Function among Traumatic Brain Injury Patients Admitted to Inten-sive Rehabilitation and Care Unit / 中国康复理论与实践

@#Objective To explore the efficacy of process management on bladder function among traumatic brain injury (TBI) patients admitted to intensive rehabilitation and care unit (IRCU). Methods 33 cases were collected to control group who were cared with routing nursing during February, 2011 to December, 2012. Other 39 cases were recruited to trial group cared with process management of bladder function during January, 2013 to December, 2014. Process management of bladder function contained the establishment of process manage-ment guide, evaluation of removing catheter, nursing of bladder based on residue of urine volume and keeping a diary of urination, etc. Re-sults There were more cases with residue of urine volume less than 100 ml and less with catheter associated urinary tract infection in the tri-al group than in the control group (P<0.05). Conclusion Process management of bladder function can improve the recovery of bladder func-tion of TBI patients, shorten rehabilitation time and decrease the risk of catheter associated urinary tract infection.

Relationship between plasma D-dimer level and diagnosis of pulmonary embolism / 心血管康复医学杂志

Objective:To explore the relationship among clinical manifestations ,SⅠ QⅢ TⅢ feature of ECG ,plasma level of D‐dimer (DD) and diagnosis of pulmonary embolism (PE) .Methods :Clinical data of 212 inpatients ,who received pulmonary CT angiography (CTA) in our hospital from Jun 2012 to May 2014 ,were retrospectively ana‐lyzed .According to pulmonary CTA results ,patients were divided into PE group (n=56) and non‐PE group (n=156) .Basic hospitalization data ,including clinical manifestations ,ECG features and plasma DD level ,were collect‐ed and compared between two groups .Results:Compared with non‐PE group ,there were significant rise in percent‐ages of dyspnea (44.87% vs .75% ) and prolonged bedridden time (3.85% vs .14.29% ) ,significant reduction in percentage of no clinical manifestations (38.46% vs .3.57% ) in PE group , P10μg/ml significantly rose (19.23% vs .32.14% ) in PE group ,P 10 μg/ml) should be considered to be PE .

The effect of infrasound on the growth of bone marrow mescenchymal stem cells / 中华物理医学与康复杂志

Objective To investigate the effect of infrasound on the growth of bone marrow mescenchymal stem cells (BMSCs) and seek the most reasonable duration for using infrasound. Methods The primary BMSCs were obtained from Sprague-Dawley rats through whole bone marrow adherent cultivation.The cells of passage 3 were divided into trial groups treated with infrasound for 10 min,30 min or 60 min,and control groups which were not treated with infrasound but exposed to air for the same durations.The vitality of cell proliferation was measured using the CCK8 method.Apoptosis and the cell cycle were analysed with flow cytometry (FACS). Results After cultivation for 72 h,the optical density (OD) values for BMSCs treated with infrasound for 10 min,30 min and 60 min were 1.480 ± 0.030,1.348 ± 0.030,1.493 ± 0.030 respectively and after 96 h they were 1.774 ± 0.030,1.731 ± 0.030 and 1.833 ± 0.030.All of which were significantly greater than in the control groups ( 1.479 ± 0.030,1.267 ±0.030 and 1.227 ± 0.030 after 72 h and 1.567 ± 0.030,1.563 ± 0.030 and 1.632 ± 0.030 after 96 h).With the extension of the treatment duration,the OD values of the BMSCs increased or increased after decreasing,and the OD value for BMSCs treated with infrasound for 60 min was the highest.The FACS results indicated no effect of infrasound on apoptosis of BMSCs when the treatment duration was 30 min,but that cell apoptosis could be inhibited when the treatment duration was 10 min or 60 min.The early phase apoptosis rates were 1.07％ ± 0.12％ and 0.97％ ±0.21％ in the trial groups treated for 10 min and 60 min respectively,and 1.43％ ± 0.06％ and 3.33％ ± 0.15％ in the respective control groups,a highly significant difference.The results of cell cycle analysis showed infrasound could disturb the cell division of BMSCs significantly when the treatment duration was less than 30 min,but there was no significant effect when the treatment duration was 60 min. Conclusions Infrasound can promote cell proliferation and disturb cell division,but it did not cause apoptosis of BMSCs.When the treatment duration was 60 min,cell proliferation improved steadily,the apoptosis rate decreased,and the cell cycle did not change.So 60 min is the most reasonable treatment duration for infrasound.

Study on the resistance-related genes of Shigella sonnei with decreased susceptibility to fluoroquinolones / 中华传染病杂志

Objective To investigate the resistance-related genes of Shigella sonnei with decreased susceptibility to fluoroquinolones.MethodsA total of 131 strains of Shigella sonnei were analyzed for their antimicrobial susceptibility.Mutations within the quinolone resistance determining regions (QRDR) of gyrA and parC were detected by polymerase chain reaction (PCR) and PCR products were then sequenced. Meanwhile, the plasmid-mediated quinolone resistance (PMQR) genes,qnr and aac(6')-Ib-cr were screened by PCR.ResultsResistance rates of 131 Shigella sonnei isolates to nalidixic acid,tetracycline,ampicillin and trimethoprim-sulfamethoxazole were 100.0％,93.9％,93.2％ and 92.8％,respectively.All strains were susceptible to norfloxacin,ciprofloxacin,levofloxacin,while 94％ nalidixic acid-resistant Shigella sonnei strains showed reduced susceptibilities to fluoroquinolones.All of nalidixic acid-resistant Shigella sonnei strains presented a single mutation at codon 83 (Ser→Leu) of gyrA genes,but no mutations were detected in parC gene.And PMQR genes qnr and aac (6’)-Ib-cr were not detected.Conclusions The nalidixic acid-resistant Shigella sonnei strains with reduced susceptibility to fluoroquinolones are common in the clinical practice,which may mainly due to a single mutation at codon 83 (Ser→Leu) of gyrA genes.

Effect of Comprehensive Rehabilitation on Acute Cerebral Infarction / 中国康复理论与实践

@#Objective To observe the effect of comprehensive rehabilitation on the motor function and activity of daily living in patients with acute cerebral infarction. Methods 110 patients with acute cerebral infarction were randomly divided into rehabilitation group and control group. The patients in control group were treated with medicine, while the patients in rehabilitation group were treated with comprehensive therapy, including medicine, physical therapy and neuromuscular electrical stimulation. They were assessed with the simplified Fugl-Meyer assessment (FMA), modified Barthel index (MBI), and the clinical defect of neurol function before and 4 weeks after treatment. Results The scores of FMA and MBI improved significantly in both groups after treatment, and improved more in rehabilitation group than in control group (P<0.05). The effective rate was 75% in the rehabilitation group, and 56% in the control group (P<0.05). Conclusion Comprehensive rehabilitation can promote the recovery of motor function and activity of daily living in patients with acute cerebral infarction.

Clinical Observation of Acupuncture using Xingnao Kaiqiao Needling Method and Rehabilitation Training on Stroke / 中国康复理论与实践

@#ObjectiveTo observe the clinical effection of acupuncture using Xingnao Kaiqiao needling method and rehabilitation training on patients with stroke.Methods70 patients with stroke were randomly divided into the observation group(n=35)and the control group(n=35), both groups receiving routine treatment and early rehabilitation training, while acupuncture using Xingnao Kaiqiao needling method was given additionally to the observation group, 4 weeks for a course. The Fugl-Meyer Assessment, assessment of neurological deficit, the Barthel Index and the clinical effect were compared among two groups.ResultsThe neurological deficit decreased obviously, and the Fugl-Meyer Assessment and Barthel Index increased significantly in two groups after the treatment(P<0.05). The theraputic effect was significantly better in the observation group than in the control group(P<0.05).ConclusionAcupuncture using Xingnao Kaiqiao needling method combined with rehabilitation training can more effectively promote the recovery of nerve function, enhance motor function, and significantly improve the quality of life and the prognosis of patients with stroke.

The effect of early rehabilitation intervention on serum neuron-specific enolase in patients with acute cerebral infarction / 中华物理医学与康复杂志

Objective To observe the effects of early rehabilitation on the serum neuron specific enolase (NSE) levels of patients with acute cerebral infarction.MethodsSixty patients with acute cerebral infarction were randomly divided into a rehabilitation group and a control group. All received routine treatment at the acute stage, including anti-platelet aggregation medication, drugs for improving microcirculation, neurotrophic agents and prompt treatment of any complications. Patients in the rehabilitation group also received systemic rehabilitation training beginning immediately after their vital signs had been stabilized. NSE in serum was assayed before treatment and after 3, 7and 14 days. National Institutes of Health stroke scale (NIHSS) scores were evaluated at each time point, and the two groups were compared.ResultsThere was no significant difference in serum NSE or NIHSS scores between the two groups pre-treatment. Both groups improved to a certain extent, but the improvements in the rehabilitation group were significantly better than in the control group, as their NSE levels at 7 days and NIHSS scores at 14 days were both significantly better.ConclusionsEarly rehabilitation intervention contributes to reducing serum NSE levels after acute cerebral infarction, lessening brain injury, and thereby promoting the recovery of damaged neural function.That may be one of the mechanisms by which early rehabilitation promotes functional recovery in patients with acute cerebral infarction.

Fall risk estimation for senile patients / 中华物理医学与康复杂志

Objective To investigate the discriminant validity and relativity of three scales applied in fall risk estimation for senile patients.Methods The timed up and go test (TUGT),the Morse Fall Scale (MFS) and the Berg Balance Scale (BBS) were used by two trained testers to evaluate the fall risk of 161 senile in-patients.The patients were divided into a falling group and a no-fall group based on their history of falling in the previous one year.Student's t-test was applied to compare the discriminant validity of the TUGT,MFS and BBS.Spearman's correlation coefficient was calculated to analyze the correlation among the three scales.Results The scores of patients in the falling group on the three scales were significantly different from those of the no-falls group.The correlation coefficients among the three scales were in the range 0.680-0.888.Conclusion The TUGT,MFS and BBS all showed high sensitivity and good discrimination in fall risks estimation for senile patients.The results with the three scales were highly correlated.Because the emphasis of these three scales is different,a suitable scale should be selected in clinical practice according to the characteristics of the senile patient.

Influence of infrasound therapy on Raji cells / 中华物理医学与康复杂志

Objective To investigate the influence of infasound therapy on Raji cells. Methods The Raji cell line was cultivated routinely and grouped as an infrasound and a control groups.Infrasound 8TM was used as a therapeutic infrasound generator which worked in 3 modes(frequency range 4～20 Hz,infrasound energy less than 90 dB).The applicator of the infasound generator was put on 1.5～2.0 cm fom the surface of liquid in the dish containing Raji ceil.Raji cells would be treated for 15,30,60,and 90 minutes,then tested with trypan blue assay,MTT assay,flow cytometry anatysis,and scanning electron microscope(SEM)after 0,24,and 48 h cultivations,respectively. Results Trypan blue assay showed that there were no significant differences between the 2 groups(P>0.05).MTT assay showed that although optical density value of the infrasound groups seemed to be lower than that of the control group.the differences were not significant(P>0.05).Flow cytometry analysis showed that the rate of necrotic cells and apoptosis cells in all groups was less than 10%;and that the differences between all groups were not significant(P>0.05).The scanning electron microscopy showed that the cells treated by infasound exposure for 120 minutes and then cuhivated for 24 h showed that the prominent or micro-floss of the membrane become shorten and decreased.The surface of the membrane became smooth. Conclusion Infrasound(less than 90 dB)treatment in the experiment had no obvious influence on multiplication and apoptosis of Raji cells.But the membrane of Raji cell Would be affected directly by the infrasound,and the penetration of the membrane could be changed.

Changes in Insulin-like growth factor-1 levels in the brains and livers of rats after cerebral ischemic injury / 中华物理医学与康复杂志

Objective To observe dynamically the expression of insulin-like growth factor(IGF-1)in the brain and liver,and to explore changes in IGF-1 levels.Methods The thread method waa used to establish middle cerebral artery occlusion in rats.The expression of IGF-1 on the ischemic side of the cerebral cortex and in the liver wag observed dynamically using immunohistochemical techniques. Results The number of IGF-1-positive cells increased significantly,peaking on the 3rd day in the cortex and the 7th day in the liver.Conclusion The expression of IGF-1 Wag upregulated in the brain after the experimental cerebral ischemia;peripheral humoral regulation of IGF-1 responded more slowly and recovered to some extent at the 7th day after cerebral ischemia.

Protective effect of pulsed magnetic field against cerebral ischemia-reperfusion injury in rats / 中华物理医学与康复杂志

Objective To examine the neuroprotective effect of pulsed magnetic field in a animal focal cere-bral ischemica-reperfusion injury model. Methods Forty-eight Sprague-Dawley (SD) rats were randomized into 3 groups, a sham-operation group, a model group and a pulsed magnetic field group, with rats 16 in each group. Mid-dle cerebral artery occlusion (MCAO) method was employed to establish the focal cerebral ischemia-reperfusion inju-ry model in rats of model group and pulsed magnetic field group. Rats in sham-operation group was subject to the same operation procedure but not underwent ischemia-reperfusion. The infarction volume, histopathological damage and expressions of IGF-1 in ischemic brain tissue were investigated to evaluate the effect of pulsed magnetic field. Results The infarction volume was reduced, histopathological damage alleviated and expressions of IGF-1 in ische-mic brain tissue elevated in the pulsed magnetic field group as compared against the model group (P<0.05). Con-clusions Pulsed magnetic field might provide neuro-protection against cerebral ischemia-reperfusion injury.

Effects of infrasound with low sound pressure level on the content of IGF-1 in brain of rats with focal cerebral ischemia-refusion injury / 中华物理医学与康复杂志

Objective To study the effects and mechanisms of infrasound with low sound pressure level on focal cerebral ischemia-refusion injury. Methods Focal cerebral ischemia was produced by 2 hours of occlusion of the middle cerebral artery in rats. lnfrasound generated by infrasound 8TM device was used as treatment factor. Thirty-two Sprague-Dawley rats were divided into three groups: sham group (n=8), model group (n=8) and in-frasound group (n=16) , and the infrasound group was subdivided into 20- and 120-min infrasound groups, with 8 rats in each group. Neurological symptoms was assessed at 2 h, 1 d, 3 d and 7 d, respectively. These rats were sacrificed after 7 days of infrasound treatment and their brains were harvested. The number of IGF-1 posive cells of ischemia cortex was counted by using immunohistochemical technique. Results Compared with model group, neurological symptoms of rats in 120-min infrasound group was significantly improved (P<0.05); the number of IGF-1 positive cells of ischemia cortex in 120-min infrasound group increased significantly as compared with that in model group (P<0.01). Conclusion Infrasound with low sound pressure level (120 min/d, 7 d) could exert neuroprotective effect in focal cerebral ischemia injury by increasing expression of IGF-1 in brain.

Protection by Jiawei Buyang Huanwu Decoction from the Harm of Infrasound (16 Hz,130 dB)in Mice / 中国康复理论与实践

@#ObjectiveTo study the effects of Jiawei Buyang Huanwu Decoction on learning and memory abilities of mice exposed to 130 dB infrasound (16 Hz). Methods80 BALB/c mice were divided into blank-control group, infrasound-control group, infrasound and drug-treated group (it was divided into 3 sub-groups according to dose). Learning and memory abilities were measured 14 d later. ResultsCompared with blank-control group, learning and memory abilities decreased (P<0.05) in infrasound-control group. Compared with infrasound-control group, learning and memory abilities increased(P<0.01) in infrasound and drug-treated group. Conclusion16 Hz,130 dB infrasound could damage learning and memory abilities. Jiawei Buyang Huanwu Decoction could protect mice from learning and memory harm exposed to the infrasound.

Detection of Virulence Factors of Vibrio parahaemolyticus by Real-Time PCR / 中华医院感染学杂志

OBJECTIVE To develop a real-time fluorescence PCR assay to detect the genes encoding thermolabile(hemolysin)(TLH),thermostable direct hemolysin(TDH) and TDH-related hemolysin(TRH) of Vibrio(parahaemolyticus).METHODS The genes of TDH and TRH were selected as target ones of thermostable direct and TDH-related hemolysin,and TLH gene as a specific genomic marker for V.parahaemolyticus.Designed and synthesized the primers and Taqman probes,we investigated 487 stool samples of doubt foodborne illness patients by real-time fluorescence PCR.RESULTS The sensitivity of the assay for TLH and TDH was 1.0?10~2copies,but the sensitivity of TRH was 1.0?10~3copies. Among 487 samples,112 V.parahaemolyticus strains were found;101 samples of these strains showed the production of TDH;none of them was positive for TRH.CONCLUSIONS The Taqman PCR is a rapid and sensitive method to detect the TLH,TDH and TRH of V.parahaemolyticus,it is well suited for screening large numbers of samples at the same time;and TDH is one of the primary virulence factors in clinical isolated V. parahaemolyticus.

Effect of floating acupuncture on the processus transverses of vertebrae lumbales Ⅲ syndrome / 中国康复理论与实践

@#ObjectiveTo explore the therapeutic effect of floating acupuncture on the the processus transverses of vertebrae lumbales Ⅲ syndrome.Methods40 patients with the processus transverses of vertebrae lumbales Ⅲ syndrome were divided into the floating acupuncture group (20 cases) and the traditional group (20 cases). The pain was evaluated with visual analogue scale (VAS) before and after treatment.ResultsCompared with traditional treatment, the floating acupuncture can relieve pain rapidly after 15 min and 1 d of treatment. ConclusionThe floating acupuncture shows preferable clinical curative effect to patients with the processus transverses of vertebrae lumbales Ⅲ syndrome.

Effect of intrathecal injection with Rocephin on intracranial infection / 中国康复理论与实践

@#ObjectiveTo observe the effect of intrathecal injection with Rocephin on intracranial infection after traumatic brain injury.Methods12 patients with intracranial infection after traumatic brain injury were treated with Rocephin, including Rocephin 2.0g vein injection twice a day and 0.1 g intrathecaluse once a day. The germiculture and drug sensitivity test were made before the treatment and antibiotic was selected.ResultsThere were 6 cases recovered, 2 cases improved markedly, 1 case improved and 3 cases unchanged. The efficient rate was 66.7%. The examination of cerebrospinal fluid showed that the levels of leucocyte and protein decreased, the glucose and chloride increased and encephalic pressure declined. 12 bacteria were verified in 12 cases and 8 bacteria were eliminated after treatment. The bacterium cleared rate was 66.7%. There were no side effects correlatives with Rocephin.ConclusionVein and intrathecal injection with Rocephin is effective on intracranial infection after traumatic brain injury.