Attitudes towards suicidal behavior and related factors in adolescent patients with depressive episode / 四川精神卫生

BackgroundAttitude towards suicide among adolescents is closely linked with their suicidal behavior. Previous studies on suicidal attitudes and related factors primarily focused on school samples， with limited research on relevant clinical samples of adolescents with depressive episode. ObjectiveTo analyze the attitudes towards suicidal behavior and the influencing factors in adolescent patients with depressive episode， so as to provide references for suicide intervention in this population. MethodsA total of 100 adolescent patients who met the diagnostic criteria for depressive episode according to the International Classification of Diseases， tenth edition （ICD-10） were selected from the outpatient and inpatient departments of the Fourth People's Hospital of Wuhu from May 2021 to July 2022. A self-designed general demographic questionnaire， Hamilton Depression Scale-17 item （HAMD-17）， Suicide Attitude Questionnaire （QSA）， Egma Minnen av Bardndosnauppforstran （EMBU） and Internet Addiction Test （IAT） were used to evaluate the patients' depressive symptoms， attitudes towards suicide， parenting styles and Internet addiction， respectively. Spearman and Pearson correlation analyses were used to examine the correlation between the factor scores of attitudes towards suicidal behavior in QSA and demographic characteristics， HAMD-17 score， EMBU score and IAT score. Multiple linear regression was conducted to screen the risk factors associated with attitudes towards suicidal behavior， and ROC curves were plotted to evaluate the predictive efficacy of each factor. ResultsThe scores on attitudes towards suicidal behavior in QSA among adolescent patients with depressive episode were positively correlated with scores on attitude towards the suicidal person， attitude towards euthanasia， as well as scores on mother's emotional warmth and understanding factor （r=0.210~0.485， P<0.05 or 0.01）. Conversely， they were negatively correlated with father's education level， suicidal behavior in the past 6 months， HAMD-17 score， IAT score， father's punishment and harshness， father's overinvolved， father's rejection and denial， father's overprotection， mother's overinvolved and overprotection， mother's rejection and denial， and mother's punishment and harshness factor scores （r=-0.571~-0.290， P<0.05 or 0.01）. Multiple linear regression analysis revealed that QSA scores on attitude towards the suicidal person （β=0.198， P<0.01） and attitude towards euthanasia （β=0.302， P<0.01） positively predicted score on attitude towards suicidal behavior， and fathers' education level （β=-0.180， P=0.043）， HAMD-17 scores （β=-0.366， P<0.01） and IAT scores （β=-0.191， P=0.030） negatively predicted scores on attitude towards suicidal behavior. The differences in predictive efficacy between the factors were not statistically significant （Z=-1.289~0.092， P>0.05）. ConclusionInternet addiction， depression severity， attitudes towards suicide， attitudes towards euthanasia and father's education level may influence attitudes towards suicidal behavior in adolescent patients with depressive episode. ［Funded by Scientific Research Project of Wuhu Municipal Health Commission in 2021 （number， WHWJ2021y076）， Bengbu Medical College Postgraduate Science and Technology Innovation Project （number， Byycxz21040）］

Combination of physician modified stent-graft fenestration and in-situ needle fenestration during thoracic endovascular aortic repair / 中华普通外科杂志

Objective:To evaluate the safety and feasibility of the in-situ needle fenestration combined with the in vitro physician modified fenestration technique to reconstruct supra-aortic branches during thoracic endovascular aortic repair (TEVAR) for aortic arch lesions requiring landing at Z0 and Z1.Methods:From Nov 2017 to Dec 2019, eighteen patients who underwent both the in-situ needle fenestration and the in vitro physician modified fenestration techniques to extend the proximal landing zone to Z0 and Z1 during TEVAR were included in our study.Results:Sixteen patients underwent in vitro physician modified fenestration ,two patients underwent in vitro physician modified fenestration to reconstruct both the left common carotid artery and the innominate artery. All eighteen patients received in-situ needle fenestration to preserve the left subclavian artery. Supra aortic branches were preserved in all patients (38/38, 100%). There was no Type Ⅰ endoleak. Type Ⅱ endoleak was found in four paitnets (4/18). Type Ⅲ endoleak occurred in one patient (1/18). Type Ⅳ endoleak in four patients (4/18). Type Ⅲ endoleak needed open aortic arch repair 6 months later. The median follow-up time was 12 months. One (1/18) died in 12 months and the other patients were doing well.Conclusions:The joint application of the in-situ needle fenestration and the in vitro physician modified fenestration to reconstruct supra-aortic branches during TEVAR for aortic arch pathologies requiring landing at Z0 and Z1 was satisfactory.

Endovascular treatment of traumatic thoracic aortic pseudoaneurysm / 中华普通外科杂志

Objective:To investigate the feasibility and safety of endovascular repair for traumatic thoracic aortic pseudoaneurysm.Methods:From Oct 2015 to Oct 2018, the clinical and followup data of 7 patients diagnosed as traumatic thoracic aortic pseudoaneurysm in Fuwai Hospital of Chinese Academy of Medical Sciences were analyzed retrospectively.Results:The patients average age was (51.2±11.0) years old. All patients underwent surgery in the hybrid operating room under general anesthesia. Two did thoracic endovascular aortic repair (TEVAR), three did TEVAR combined with chimney technique to reconstruct the left subclavian artery, and 1 had TEVAR combined with fenestration to reconstruct the left subclavian artery. One did TEVAR with left common carotid artery and left subclavian artery bypass. The mean operative time was (90.1±27.4) min, the mean postoperative hospital stay was (8.9±3.7) d, and the mean postoperative follow-up time was 42.4 months. All the patients received CTA reexamination of the aorta after 1, 6, 12 months and yearly thereafter. TypeⅠendoleak was found in one patient with chimney technique to reconstruct of left subclavian artery after operation. CT showed that the type Ⅰ endoleak disappeared 6 months after operation. There was no death, paraplegia or stroke during the perioperative period and follow-up period, and there was no aortic related reintervention.Conclusion:TEVAR is a safe and effective method for the treatment of traumatic pseudoaneurysm of thoracic aorta, and the early and mid-term results were satisfactory.

Comparison of choroid thickness in healthy pregant women and non-pregant women / 中华眼底病杂志

Objective:To compare and observe the changes in choroidal thickness between healthy pregnant women and healthy non-pregnant women.Methods:A prospective clinical study. From January 2019 to August 2019, healthy pregnant women (pregnant women group) and healthy non-pregnant women age-matched were enrolled during the same period (the normal group) in the obstetrics of Zhuji People's Hospital. All patients were enrolled with their right eyes. Frequency-domain OCT-enhanced depth imaging technology was used to measure the subfoveal macular and 1000 μm above, below, nasal, and temporal choroidal thickness and foveal retinal thickness (CMT). The choroidal thickness and CMT of the pregnant women group and the normal group were compared by t test, and the choroidal thickness and CMT of the normal group and the eyes of different gestational weeks were compared by one-way analysis of variance. Results:The pregnant women group and the normal group included 161 patients (161 eyes) and 40 patients (40 eyes). According to the different gestational weeks, the pregnant women were divided into the first trimester group, the second trimester group, and the third trimester group, with 47 patients (47 eyes), 66 patients (66 eyes), and 48 patients (48 eyes) respectively. There was no significant difference in age, axial length, intraocular pressure, and CMT between the different gestational week groups and the normal group ( F=1.433, 1.558, 0.416, 2.288; P>0.05). The subfoveal choroidal thickness (SFCT) of the pregnant women group and normal group were 317.7±73.9 μm and 279.7±44.1 μm, respectively, and the difference was statistically significant ( t=3.113, P=0.002). Compared with the normal group, the choroid at the upper, lower, nasal, and temporal sides of the pregnant group 1000 μm from the fovea was thickened. The difference between the upper, nasal and temporal sides was statistically significant ( t=2.699, 3.474, 2.595; P<0.05). The SFCT of the eyes in the first trimester group, the middle group, and the late group were 305.8±72.3, 327.7±69.8, 315.8±80.5 μm, respectively. Compared with the normal group, the difference was statistically significant ( F=4.180, P=0.007). Pairwise comparison between the two groups, the second trimester group was significantly different from the normal group ( P=0.003). There was no significant difference among the first trimester group、the third trimester group and the other groups ( P>0.05). Conclusion:The choroidal thickness of pregnant women is thicker than normal, and the choroidal thickness in the second trimester reaches the maximum value; while the macular CMT during pregnancy has no significant change.

Myeloid/lymphoid neoplasms with eosinophilia and FGFR1 rearrangement: 5 cases report and literatures review / 中华血液学杂志

Objective@#To investigate the clinic-pathological features, diagnosis and treatment of 8p11 myeloproliferative syndrome (EMS) .@*Methods@#Five patients diagnosed as EMS from Jan 2014 to May 2018 at Blood Disease Hospital, Chinese Academy of Medical Sciences were enrolled. The clinical manifestations, laboratory characteristics, treatment and outcome of these patients were summarized.@*Results@#The peripheral blood leukocyte count of 5 patients with EMS increased significantly, accompanied with an elevated absolute eosinophils value (the average as 18.89×109/L) . The hypercellularity of myeloid cells was common in bone marrow, always with the elevated proportion of eosinophils (the average as 17.24%) , but less than 5% of blast cells. The chromosome karyotype of the 5 cases differed from each other, but presenting with the same rearrangement of FGFR1 gene by fluorescence in situ hybridization technology. The average interval between onset and diagnosis was 4.8 months with a median survival of only 14 months.@*Conclusion@#EMS was a rare hematologic malignancy with poor prognosis and short survival. It was commonly to be misdiagnosed. Analysis of cytogenetics and molecular biology were helpful for early diagnosis.

Cytogenetic test and clinical study on cryptic acute promyelocytic leukemia with ins (15; 17) / 中华血液学杂志

Objective@#To investigate the genetic screening methods for cryptic acute promyelocytic leukemia (APL) to further explore its clinical prognosis.@*Methods@#From June 2016 to November 2018, we collected 373 newly diagnosed APL cases. The patients were retrospected by the results of PML-RARα detections both by RT-PCR and i-FISH, those who harbored positive PML-RARα detection by RT-PCR and negative by i-FISH were chosen. Metaphase FISH and Sanger sequencing were further performed to verify these results.@*Results@#A total of 7 cryptic APL cases were discovered. These cases had tiny fragment of RARα inserted into PML in chromosome 15, formed ins (15;17) . The 7 cryptic APL cases had no PML-RARα gene subtype specificity, involving 5 cases in L subtype, 1 case in S subtype and 1 case in V subtype respectively. After the treatment of retinoic acid and arsenic or anthracyclines, 6 cases achieved complete remission, 1 case died of intracranial hemorrhage on the 6th day of therapy.@*Conclusion@#The size and covering position of PML-RARα probe should be taken into account when PML-RARα was performed by FISH on APL patients. Furthermore, combination with Metaphase FISH could improve the recognition of cryptic APL. There were no differences between the cryptic and common APL patients in terms of clinical features and treatment choices. Cryptic APL patients also had a good response to the therapy of retinoic acid and arsenic or anthracyclines.

The consistency and reproducibility of macular perfusion parameters in early diabetic retinopathy using optical coherence tomography angiography / 中华眼底病杂志

Obejctive To investigate the consistency and reproducibility ofmacular perfusion parameters in early diabetic retinopathy (DR) using optical coherence tomography angiography (OCTA).Methods A prospective cross-sectional observational study.Forty-six patients (46 eyes) diagnosed with mild nonproliferative DR were included in this study.There were 24 males and 22 females,with the mean age of 59.16± 10.32 years.Two macular scan sizes of 3 mm× 3 mm and 6 mm× 6 mm were performed by the same operator,and the same test was performed by another operator.The superficial retinal layer (SRL) and deep retinal layer (DRL) in the foveal avascular zone (FAZ) and vessel density (VD) were quantified.The consistency of the two scan sizes and the reproducibility of the same scan size were also evaluated.The consistency was determined by the intraclass correlation coefficient (ICC).If the intraclass correlation coefficient (ICC)＞0.80,consistency was good;if 0.4≤ICC＜0.8,consistency was general;if ICC＜0.40,consistency was poor.Results In the 3 mm × 3 mm and 6 mm × 6 mm scanning sizes,the mean results of the two examiners were calculated.The FAZ of SRL were 0.39±0.13 mm2 and 0.42±0.15 mm2,FAZ of DRL were 0.74±0.22 mm2 and 0.89±0.23 mm2.The VD of SRL were (32.23±2.86)％ and (31.91 ±3.01)％,VD of DRL were (43.73 ±4.64)％ and (45.12± 5.49)％.The consistency analysis showed that the ICC of SRL-FAZ and DRL-FAZ were 0.920 and 0.812,respectively;the ICC of VD were 0.833 and 0.830,respectively.The consistency was good.The reproducibility analysis of different examiners in the same scan size was better in the consistency of SRL FAZ and VD.Conclusion OCTA in two scanning sizes to measure FAZ and VD of early DR has good consistency and reproducibility.

The change of central retinal thickness and subfoveal choroidal thickness in patient with central retinal artery occlusion during short-term treatment / 中华眼底病杂志

Objective To observe the center retinal thickness (CRT) and subfoveal choroidal thickness (SFCT) in eyes with central retinal artery occlusion (CRAO) before and after treatment.Methods A total of 34 patients (34 eyes) diagnosed with CRAO by fundus fluorescence angiography (FFA) were retrospectively analyzed.There were 18 males (18 eyes) and 16 females (16 eyes).The average age was (61.42± 14.09) years.The mean onset time was (2.64±3.73) days.The mean hospitalization time was (11.92 ±4.95) days.The mean axial length (AL) was (23.53 ± 2.04) mm.The best-corrected visual acuity (BCVA),slit-lamp biomicroscopy,indirect ophthalmoscopy,fundus color photography,fundus fluorescein angiography,spectral domain optical coherence tomography (OCT) and AL measurement were performed.BCVA was converted to the logarithm of the minimum angle of resolution (logMAR).According to FFA,visual loss and the results of OCT,patients were divided into 3 groups:incomplete CRAO (15 eyes),subtotal CRAO (8 eyes),total CRAO (11 eyes).SFCT and CRT in affected and the fellow eye were measured by OCT for enhanced deep imaging.Follow up lasted for 1 month after treatment,with an average follow-up of (34.71 ± 6.82) days.The changes of S FCT,CRT,and BCVA before and after treatment were observed.The correlation between BCVA after treatment and pretreatment CRT was also analyzed.Results After 1 month of follow-up,the logMAR BCVA in incomplete group,subtotal group and total group were significantly higher than before treatment (t=3.74,3.61,3.26;P=0.004,0.009,0.017).Before treatment,the average CRT of the contralateral eyes in the total,subtotal and incomplete group were (215.00± 19.85),(224.00±22.79),(214.00±8.21) μtm,and the mean SFCT were (264.54± 121.71),(266.50±58.17),(261.86±90.95) μm.The average CRT of the affected eyes were (353.18±60.26),(280.14± 11.08),(266.63±19.65) μtm,and the average SFCT were (233.72± 111.35),(237.75 ± 53.30),(259.86±98.14) mm.Compared with the fellow eyes,the average CRT in the 3 groups were thickened,and the difference were statistically significant (t=8.274,3.694,11.577;P＜0.001,0.008,＜0.001);the average SFCT in the total group was decreased,the difference was statistically significant (t=-2.138,P=0.048).The mean CRT among the 3 groups of eyes was statistically significant (F=1 2.02,P＜ 0.001).There was no significant difference in the average SFCT (F=0.178,P=0.838).After 1 month follow-up,the mean CRT in the total,subtotal and incomplete group were (231.18 ± 49.28),(219.16± 21.34),(217.86± 24.98) μm,and the average SFCT were (239.81 ± 109.57),(241.86±42.81),(260.57±91.67) μan.Compared with before treatment,the average CRT in the three groups of eyes were decreased,the difference were statistically significant (t=13.032,3.711,4.970;P＜0.001,0.008,0.003);the difference in mean SFCT were not statistically significant (t=-0.785,-0.202,-0.078;P=0.466,0.845,0.940).Correlation analysis showed that BCVA after treatment was positively correlated with pretreatment CRT (odds ratio=0.578,P=0.002).Conclusion CRAO resulted in CRT in the preliminary stage and became thinner after receiving treatments.There exists a positive correlation between visual outcome and CRT before receiving treatments.

Characteristics and prognosis in adult acute myeloid leukemia patients with MLL gene rearrangements / 中华血液学杂志

Objective@#To analyze the clinical and laboratory characteristics, and prognosis of adult acute myeloid leukemia (AML) patients with MLL gene rearrangements.@*Methods@#The medical records of 92 adult AML patients with MLL gene rearrangements from January 2010 to December 2016 were retrospectively analyzed.@*Results@#92 cases (6.5%) with MLL gene rearrangements were identified in 1 417 adult AML (Non-M3) patients, the median age of the patients was 35.5 years (15 to 64 years old) with an equal sex ratio, the median WBC were 21.00(0.42-404.76)×109/L, and 78 patients (84.8%) were acute monoblastic leukemia according to FAB classification. Eleven common partner genes were detected in 32 patients, 9 cases (28.1%) were MLL/AF9(+), 5 cases (15.6%) were MLL/AF6(+), 5 cases (15.6%) were MLL/ELL(+), 2 cases (6.3%) were MLL/AF10(+), 1 case (3.1%) was MLL/SETP6(+), and the remaining 10 patients’ partner genes weren’t identified. Of 92 patients, 83 cases with a median follow-up of 10.3 (0.3-74.0) months were included for the prognosis analysis, the complete remission (CR) rate was 85.5% (71/83), the median overall survival (OS) and relapse free survival (RFS) were 15.4 and 13.1 months, respectively. Two-year OS and RFS were 36.6% and 29.5%, respectively. Of 31 patients underwent allogeneic hematopoietic stem-cell transplantation (allo-HSCT), two-year OS and RFS for patients received and non-received allo-HSCT were 57.9% and 21.4%, 52.7% and 14.9%, respectively (P<0.001). Among patients with partner genes tested, 9 of 32 cases (28.1%) were MLL/AF9(+), the median follow-up was 6.0(4.1-20.7) months. 3 patients with MLL/AF9 underwent allo-HSCT. 23 cases (71.9%) were non- MLL/AF9(+), the median follow-up was 7.8 (0.3-26.6) months. 14 patients (60.1%) with non-MLL/AF9 underwent allo-HSCT. One-year OS for patients with MLL/AF9 and non-MLL/AF9 were 38.1% and 55.5%, respectively (P=0.688). Multivariate analysis revealed that high WBC (RR=1.825, 95% CI 1.022-3.259, P=0.042), one cycle to achieve CR (RR=0.130, 95% CI 0.063-0.267, P<0.001), post-remission treatment with allo-HSCT (RR=0.169, 95% CI 0.079-0.362, P<0.001) were independent prognostic factors affecting OS.@*Conclusions@#AML with MLL gene rearrangements was closely associated with monocytic differentiation, and MLL/AF9 was the most frequent partner gene. Conventional chemotherapy produced a high response rate, but likely to relapse, allo-HSCT may have the potential to further improve the prognosis of this group of patients.

Effects of Si-Wu Decoction and its active components on cytochrome P450 in rats / 中国药理学通报

Aim To study the influence of Si-Wu De-coction (SWD ) and its active components on cyto-chrome P450 activity and mRNA expression in rats in order to provide an experimental basis for compatibility of SWD.Methods SWD and its active components were intragastrically administrated for seven days,the doses of SWD was 10 g · kg -1 · d -1 ,the doses of fructose,ferulic acid,ligustrazine,peoniflorin were 0.334,0.002,0.011 and 0.022 g·kg -1 ·d -1 ,re-spectively.After administration for seven days,rats were executed,and liver microsomes were prepared. The effects of SWD and its active components on cyto-chrome P450 in rats were investigated by hybrid probe and liver microsomes incubation method.The level of mRNA expression in liver was detected by real-time quantitative polymerase chain reaction using specific target primers for CYP450 genes.The level of protein expression of CYP2B1 was detected by Western blot. Results Compared with the control group,fructose significantly decreased the activity of CYP1A2, CYP2B6,CYP2C9,CYP2D6;ferulic acid significantly decreased the activity of CYP2C9,CYP2B6;ligus-trazine significantly decreased the activity of CYP1A2, CYP2C9,CYP2B6;peoniflorin significantly decreased the activity of CYP2D6,CYP2B6;fructose,ferulic acid,peoniflorin inhibited the mRNA expression of CYP2B1;fructose,ferulic acid,ligustrazine and peon-iflorin also inhibit the protein expression of CYP2B1. Conclusion Fructose,ferulic acid,peoniflorin inhib-it the activity of CYP2B1,decrease the expression lev-els of mRNA and protein of CYP2B1.

Mitochondrial toxicity effect of Radix Aconiti Lateralis Praeparata on H9c2 cardiomyocytes / 中国药理学与毒理学杂志

OBJECTIVE To study the mitochondrial toxicity effect of Radix Aconiti Lateralis Praepa?rata(Fuzi)on H9c2 cardiomyocytes. METHODS H9c2 cells were exposed to Fuzi decoction 6.25, 12.5,25,50 and 100 g·L-1 for 24 h. Fluorescence staining and CCK-8 assay were used to detect cell viability. H9c2 cells were exposed to Fuzi decoction 6.25,12.5 and 25 g · L-1 for 24 h,while the effect on mitochondrial membrane potential and reactive oxygen species(ROS)was detected by flow cytometry. The fluorescence molecular probe and laser scanning confocal microscope were used to observe the effect on Ca2+ in cells,Ca2+ and superoxide in mitochondria. The effect on ATP concentration in cells was detected via firefly luciferin and the expression of Pgc-1α,Bcl-2 and Bax mRNA evaluated by real-time PCR,while the expression of Pgc-1α protein was measured by Western blotting. RESULTS H9c2 cell viability was significantly inhibited by Fuzi decoction 12.5-100 g · L-1(P<0.05,P<0.01). The IC50 value was 47.4669 g · L-1,while the 95%confidence limit was 32.5997-69.1145 g · L-1. After treatment with Fuzi decoction 25 g · L-1 ,the fluorescence intensity of ROS in the normal control group increased from 204±67 to 454±78(P<0.05),that of mitochondrial superoxide increased from 5.4±1.8 to 26.8±8.5 (P<0.01),mitochondrial membrane potential decreased from 1.7±0.5 to 0.8±0.4(P<0.05),the fluores?cence intensity of intracellular Ca2+increased from 7.8±0.8 to 22.1±0.5(P<0.05)while that of mitochon?drial Ca2+decreased from 38.0±4.3 to 9.2±1.6(P<0.01),and intracellular ATP concentration decreased from (10.6 ± 0.4)μmol · g-1 to (5.3 ± 1.1)μmol · g-1 protein (P<0.05). qPCR and Western blotting test results showed that compared with the normal control group ,Pgc-1αand Bcl-2 mRNA relative expression level in Fuzi decoction 25 g·L-1 group was decreased from 1.00±0.10 and 1.00±0.10 to 0.09±0.06(P<0.01)and 0.43±0.06(P<0.01),respectively, while the relative expression of Bax mRNA was increased from 1.00 ± 0.03 to 1.17 ± 0.06 (P<0.05),and the expression of Pgc-1α protein was decreased from 0.906±0.034 to 0.541±0.003(P<0.01). CONCLUSION Fuzi has some mitochondrial toxicity to cardiomy?opathy. This effect arises from the combined action of different mechanisms. Mitochondrial toxicity of myocytes may account for the cardiac toxicity of Fuzi.

Mortalin promotes ovarian cancer cell growth through MAPK-ERK signal pathway / 解剖学报

Objective By constructing mortalin stably expressing ovarian cancer cell lines in A 2780 and A2780/cis, we demonstrate the role of mortalin in the ovarian cancer cell growth .Methods CCK-8 assay was used to measure cell viability in the overexpression mortalin group compared with the control group .The flow cytometry analysis was used to understand the effect of upregulated mortalin on the ovarian cancer cell cycle .Western blotting was used to determine the expression and phosphorylation level of MAPK /ERK and JNK/SAPK signal pathways .Results The results showed that increased expression of mortalin could accelerate ovarian cancer cell proliferation and promote G 1 transition, leading to a faster restoration of normal distribution of cell cycle .We found that mortalin overexpression significantly activated p-Raf and p-ERK1/2, but not p-JNK.Conclusion The results demonstrate that mortalin effect on the ovarian cancer cell proliferation contributes to active the MAPK-ERK signaling pathway .

Application of three-probe fluorescence in situ hybridization panel in the diagnosis of pediatric B cell acute lymphoblastic leukemia / 中华血液学杂志

<p><b>OBJECTIVE</b>To evaluate the three-probe fluorescence in situ hybridization (FISH) panel in the diagnosis of pediatric B cell acute lymphoblastic leukemia (B-ALL).</p><p><b>METHODS</b>Three-probe (TELAML1, BCR-ABL and MLL) FISH and conventional cytogenetic analysis were performed in 207 children with B-ALL.</p><p><b>RESULTS</b>In 207 B-ALL children, the three-probe FISH panel assay showed that 151 cases carried genetic aberrancies with a positive rate of 72.9%, including 44 cases with typical positive signal patterns and 148 cases with atypical signal patterns (among them 41 cases have multiprobe abberancy). The conventional cytogenetic analysis detected 53 cases chromosomal abnormality with a positive rate of 25.6%.</p><p><b>CONCLUSION</b>The detection rate of genetic abnormalities in newly- diagnosed pediatric B-ALL could be significantly improved by using three-probe FISH panel upon conventional cytogenetic analysis.</p>

Increased expression and possible roles of nicotinamide N-methyltransferase in pancreatic islets of STZ-induced diabetic monkeys / 中国病理生理杂志

AIM: To verify and localize the expression of nicotinamide N-methyltransferase (NNMT) in pancreas of streptozotocin(STZ)-induced diabetic monkeys and understand its important role in ?-cell destruction in the pathogenesis of diabetes. METHODS: Through an olig-microarray gene chip, NNMT was identified as the most obviously up-regulated genes in pancreas of STZ-induced diabetic monkeys versus controls. Semiquantitative RT-PCR and Western blotting were performed to verify the differential expression at mRNA and protein level respectively. Then the cellular localization of NNMT expression within pancreas was identified by immunohistochemical(IHC) staining.RESULTS: An obvious high expression of NNMT at both mRNA and protein levels was shown in pancreas of STZ-induced diabetic monkeys compared to that of controls. Further localization of the protein by IHC staining in pancreas specimens showed that its altered expression was restricted to central islets, most of which were ? cells.CONCLUSION: Expression of NNMT is increased in islets of STZ- induced diabetic monkeys, which infers that NNMT might participate in the process of ? cell damage in diabetes probably through the mechanism of energy metabolism disturbance.

Insulin resistance of islet cells: the central role of pathogenesis in type 2 diabetes? / 中华内分泌代谢杂志

Systemic insulin resistance has been well known. Recently, an important question has been raised regarding whether islet cells are resistant to insulin (insulin resistance of ?, ? and ? cells) due to the discovery of insulin receptor and its downstream insulin signaling pathways in islet cells. As a new study field of insulin resistance, the elucidation to this issue is crucial for the new insight into the mechanisms of insulin resistance per se and type 2 diabetes.

REGULATION OF ?-7 NEURONAL NICOTINIC ACETYLCHOLINE RECEPTORS BY NICOTINE IN MIDBRAIN OF RATS / 解剖学报

Objective To observe the regulation of ?-7 neuronal nicotinic acetylcholine receptors(?-7-nAChRs) by nicotine in the ventral tegmental area(VTA),the substantia nigra(SN) and the cortex of the rat. Methods Nicotine exposed animal models were established.The changes of ?-7-nAChRs proteins and mRNA were observed by immunohistochemistry and in situ hybridization in VTA,SN and cortex,and the expression of ?-7-nAChRs proteins by Western blotting in PC12 cells. Results The expression of ?-7-nAChRs proteins in cortex and mRNA in VTA and SN were upregulated by nicotine.The expressions of ?-7-nAChRs in PC12 cells were in proportion to and dependent on the time for nicotine to function and the dose.Conclusion Both ?-7-nAChRs mRNA in VTA and SN in transcriptional mechanism and ?-7-nAChRs protein in the cortex at the translation level were upregulated.