Mechanism of action of glucocorticoid receptors in the growth of hepatoma cells / 国际肿瘤学杂志

The glucocorticoid receptor (GR) plays a critical role in signal transduction, expression of related genes and apoptosis of cancer cells. Hepatocellular carcinoma is characterized by high aggressiveness and mortality. In the exploration of the relationship between GR and hepatocellular carcinoma, numerous studies have shown that GR has an inhibitory effect on the growth of hepatocellular carcinoma cells, which provides new ideas and methods for the clinical application of GR in the treatment of hepatocellular carcinoma.

Genetic analysis of a child with Pitt-Hopkins syndrome due to a novel variant of TCF4 gene derived from low percentage maternal mosaicism / 中华医学遗传学杂志

OBJECTIVE@#To explore the genetic etiology of a child with Pitt-Hopkins syndrome.@*METHODS@#A child who had presented at the Medical Genetics Center of Gansu Provincial Maternal and Child Health Care Hospital on February 24, 2021 and his parents were selected as the study subjects. Clinical data of the child was collected. Genomic DNA was extracted from peripheral blood samples of the child and his parents and subjected to trio-whole exome sequencing (trio-WES). Candidate variant was verified by Sanger sequencing. Karyotype analysis was also carried out for the child, and her mother was subjected to ultra-deep sequencing and prenatal diagnosis upon her subsequent pregnancy.@*RESULTS@#The clinical manifestations of the proband included facial dysmorphism, Simian crease, and mental retardation. Genetic testing revealed that he has carried a heterozygous c.1762C>T (p.Arg588Cys) variant of the TCF4 gene, for which both parents had a wild-type. The variant was unreported previously and was rated as likely pathogenic based on the guidelines of the American College of Medical Genetics and Genomics (ACMG). Ultra-deep sequencing indicated that the variant has a proportion of 2.63% in the mother, suggesting the presence of low percentage mosaicism. Prenatal diagnosis of amniotic fluid sample suggested that the fetus did not carry the same variant.@*CONCLUSION@#The heterozygous c.1762C>T variant of the TCF4 gene probably underlay the disease in this child and has derived from the low percentage mosaicism in his mother.

A study comparing PTCD with ENBD on jaundiced patients before laparoscopic pancreaticoduodenectomy / 中华肝胆外科杂志

Objective:To compare and analyze the perioperative outcomes of jaundiced patients undergoing laparoscopic pancreaticoduodenectomy (LPD) using preoperative percutaneous transhepatic cholangial drainage (PTCD) versus endoscopic nasobiliary drainage (ENBD).Methods:The perioperative data of 173 patients who underwent LPD at the Second Hospital of Hebei Medical University from January 2016 to December 2020 and were treated preoperatively with either PTCD versus ENBD to alleviate jaundiced were retrospectively analyzed. There were 100 males and 73 females, with age of (60.4±10.8) years old. These patients were divided into the PTCD group ( n=126) and the ENBD group ( n=47). Clinical data including operation time, blood loss, transfusion volume, R 0 resection, and postoperative complications were compared. Results:There was no convension to open surgery. There were no significant differences in operation time, blood loss, transfusion volume, R 0 resection rate, pathological results and hospital stay between the two groups ( P>0.05). For the PTCD group, the pancreatic fistula rate was 10.3% (13/126) and the post-operative hemorrhage rate was 8.7% (11/126). They were both significantly lower than those of the ENBD group [25.5% (12/47) and 25.5% (12/47) respectively, P<0.05]. There were also significant differences in the postoperative complications according to the Clavien-Dindo classification system between the two groups ( P=0.008). Conclusion:Compared with ENBD, PTCD had the advantages of lower post-operative pancreatic fistula and post-operative hemorrhage rates, resulting in a better postoperative recovery.

Effect of occupational vibration on occupational psychological behavior of workers / 中华行为医学与脑科学杂志

Objective:To explore the influence of vibration operation on occupational psychological behavior of workers.Methods:From May to August 2021, a total of 384 workers from 20 factories in a city of Guangdong Province were selected as the research objects.According to the workers whether engaged in vibration operations, they were divided into the vibration group and the control group.There were 229 workers in the vibration group and 155 workers in the control group.The occupational stress scale, job burnout scale and job fatigue symptom scale were used for questionnaire survey, and IBM SPSS 24.0 software was used for statistical description and data analysis, and t-test was used for comparison of independent samples between the two groups, one-way ANOVA was used for comparison of means between multiple groups, and LSD test was used for further pin-pide comparison. Results:In the vibration group, the scores of cognitive behavioral stress(3.78±1.79), psychological stress response(4.15±2.29), physical stress(2.81±1.65) and total stress (10.74±5.11) were higher than those of the control group ((2.23±1.23), (2.30±1.62), (1.81±1.07), (6.34±2.88), t=9.31, 8.67, 6.68, 9.72, all P<0.01), and job burnout scale of emotional exhaustion (8.95±3.62), depersonalization (3.24±2.16) were higher than the control group ((5.37±2.72), (1.79±1.20), t=10.48, 7.59, both P<0.01). The score of personal achievement (26.20±2.47) was lower than that of control group ((29.73±2.52), t=13.64, P<0.01). In the job fatigue symptom scale of sleepiness (8.38±1.52), emotional restlessness (6.85±1.74), displeasure (6.38±1.57), sluggishness (8.94±2.05), visual fatigue (7.83±1.95), and total score (38.37±6.56) were higher than those of the control group ((7.89±1.29), (6.21±1.32), (5.61±1.29), (6.54±1.52), (6.67±2.02), (32.92±5.56), t=3.26, 3.90, 5.00, 12.45, 5.61, 8.48, all P<0.01)). There were significant differences in scores of occupational stress, occupational burnout and occupational fatigue symptom among different seniority groups (all P<0.01). The scores of occupational stress, job burnout and job fatigue symptoms of workers in 6-10 years and more than 10 years of service group were higher than those in 0-5 years of service group, and the differences were statistically significant ( P<0.05). Length of service was correlated with occupational stress, job burnout and job fatigue.There was a correlation between the scores of all dimensions and total score of the occupational stress scale and the scores of all dimensions of occupational burnout scale ( r=-0.67-0.63, P<0.01), as well as the scores of all dimensions and total score of the occupational fatigue symptom scale ( r=0.26-0.73, P<0.01). The direct effect of occupational stress on job fatigue was 0.417, and the mediating effect of occupational stress on job fatigue through job burnout was 0.127. Conclusion:Vibration work can lead to occupational stress, job burnout and job fatigue, which is an important factor affecting occupational psychology and occupational behavior.

Application value of three dimensional imaging fusion navigation system in laparoscopic pan-creatic surgery / 中华消化外科杂志

Objective:To investigate the application value of three dimensional (3D) imaging fusion navigation system (hereinafter referred as navigation system) in laparoscopic pan-creatic surgery.Methods:The retrospective and descriptive study was conducted. The clinical data of two volunteers, including volunteer 1 undergoing laparoscopic pancreaticoduodenectomy for cholangiocarcinoma and volunteer 2 undergoing laparoscopic pancreaticotomy + splenectomy for pancreatic cancer, who were admitted to the Second Hospital of Hebei Medical University from December 2020 to May 2021 were collected. The 2 volunteers were females, aged 68 years and 40 years, respectively. The self-developed navigation system was applied in laparoscopic simulator model test, including in rigid-body model and prosthesis model, and clinical test. Observation indicators: (1) results of model test; (2) results of clinical test. Measurement data with normal distribution were represented as Mean± SD. Results:(1) Results of model test. The rigid-body model or prosthesis model with occlusion can be seen in the laparoscopic visual field of the initial laparoscopic simulator. The rigid-body model or prosthesis model with occlusion and rigid-body model or prosthesis model without occlusion can be seen in the 3D visual reconstruction image of enhanced computer tomography (CT) examination. The rigid-body model or prosthesis model with occlusion can be seen in the laparoscopic visual field of the initial laparoscopic simulator after the 3D visual reconstruction image was superimposed and fused with the real-time laparoscopic image. Both of the rigid-body model and prosthesis model were in high consistency, with the distance error of marker points were (0.26±0.11)mm and (0.29±0.18)mm, respectively. (2) Results of clinical test. The abdominal organs and blood vessels with occlusion of the 2 volunteers can be seen in the initial laparoscopic visual field. The location of tumor, important organs and blood vessels can be seen in the navigation system using the 3D visual reconstruction image of enhanced CT examination. The location of tumor, important organs and important blood vessels can be seen in the laparoscopic visual field after the 3D visual reconstruction image was superimposed and fused with the real-time laparoscopic image. The distance error of marker points of the volunteer 1 was (1.36±0.57)mm and the distance error of marker points of the volunteer 2 was (1.24±0.33)mm.Conclusions:The self-developed navigation system can integrate the preoperative 3D visual reconstruction image of enhanced CT examination and the intraoperative real-time laparoscopic image with a good effect. The relationship between deep tissue and blood vessels which is not clarified in conventional laparoscopy can be shown in the navigation system assisted laparoscopic pancreatic surgery.

Identification of epitope recognized by a monoclonal antibody against VP2 protein of bluetongue virus serotype 8 / 生物工程学报

To confirm the B cell epitope recognized by monoclonal antibody (MAb) 3G11 of bluetongue virus type 8 (BTV-8) VP2 protein prepared in our laboratory, antigen epitopes recognized by 3G11 were screened and identified by phage display technology. KLLAT sequence was found by sequencing of blue spot after four rounds panning and 283LL284 of common short peptide sequence was obtained after comparison to amino acid sequence of BTV-8 VP2 protein. The peptide sequences KLLAA, KALAT, KLAAT and KLLAT were synthesized and identified by indirect ELISA. KLLAA and KLLAT bound strongly with supernatant and as cites of 3G11 cells and reacted specifically with BTV-8 positive standard sera. Further sequence analysis showed that amino acid sequence 283LL284 was conserved among different serotypes of BTV-8 strains, and283LL284 was the key amino acids of antigen epitopes recognized by 3G11. This study laid the foundation to establish type 8 BTV specific immunological detection methods.

Establishment of two competitive ELISAs for specific detection of bluetongue virus serotype 4 / 生物工程学报

To develop a clinical diagnosis technique for bluetongue virus infection, we established serotype-specific methods to detect serotype 4 of bluetongue virus (BTV-4). Two monoclonal antibodies (mAbs) against VP2 protein of BTV-4, named 4A-1G7 and 4B-1B6, were used as competitive antibodies in the competitive enzyme-linked immunosorbent assays (C-ELISA). We detected 50 negative serum samples from sheep, goats and cattle by C-ELISA. The cut-off values of 4A-1G7 and 4B-1B6 mAbs were 49% and 40%, respectively. The results of the sensitivity, specificity and repeatability by detecting standard positive serum, were consistent with the general standard of Office International Des Epizooties. Furthermore, serum samples of BTV-4, BTV-18 and BTV-20 infection could be screened out through the combined C-ELISAs by 4A-1G7 and 4B-1B6 mAbs. Thus, this technique may diagnose BTV-4, BTV-18 and BTV-20 infections.