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Artigo em Chinês | WPRIM | ID: wpr-821296

RESUMO

Objective@#To investigate the effects of miRNA on the expression of paraoxonase 1 (PON1) and its clinical application in the patients with nonalcoholic steatohepatitis (NASH). @*Methods@#Bioinformatics methods were used to analyze and predict PON1 related regulation on miRNA. PON1 luciferase reporter gene vectors were constructed and the activity of dual luciferase was analyzed. The up/down-regulated levels of miRNA in HepG2 cells of different groups were detected by real-time fluorescence quantitative PCR (qRT-PCR), and the levels of PON1 protein in HepG2 cells were detected by western blot. The levels of miR140-5p in the serum of healthy people and NASH patients were also analyzed by qRT-PCR. @*Results@#According to the prediction of TargetScan database, miR140-5p may bind complementarily to the end of PON13′-UTR. The analysis for the activity of dual luciferase reporter gene showed that miR-140-5p mimic significantly downregulated the fluorescence of wild type PON1 vector (P<0.01). The results of qRT-PCR demonstrated that miR-140-5p mimic group showed high overexpression (P<0.01) compared with the normal cell control group and the negative mimic control group, while miR-140-5p inhibitor group appeared corresponding low expression (P<0.05). western blot results suggested that the transfection of miR140-5p mimic significantly down-regulated the expression of PON1 (P<0.01) while miR140-5p inhibitor up-regulated this expression (P<0.01). Compared with the healthy control group, the level of miR140-5p was decreased in the serum of NASH patients, and the difference was statistically significant (P<0.01). @*Conclusion@#miR140-5p may be involved in the progression of nonalcoholic steatohepatitis through regulation for the posttranscriptional gene expression of PON1.

2.
Artigo em Chinês | WPRIM | ID: wpr-567056

RESUMO

Objective To study the diagnostic value of detecting pre-albumin,albumin and protein electrophoresis in the serum of patients with fever of unknown origin.Methods Serum pre-albumin,albumin and protein electrophoresis were detected in 86 patients with fever of unknown origin and 26 controls.Results The level of albumin and pre-albumin in fever of unknown origin group was lower than that of control group(P

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