RESUMO
Objective The present study was designed to investigate whether pulsing DCs with tumor-derived extracts is an ef- fective way to induce CTL. and antitumor immunity, Methods DCs were propagated from bone marrow (BM)of C57BL/6J(H-2Kb. I- Ab)mice in vitro with GM-CSF + IL-4tumor associated antigen (TAA) extracted from actively growing Hepa 1-6 cells was used to activate DCs. The phenotypes of DCs were detected by FACS, the cytotoxicity of CTL was as- sayed by 3H-TdR labbel assay. Result and Conclusion The TAA extract pulsed DCs exhibited much more and longer cell processes and increased expression of MHC- Ⅰ, MHC-Ⅱ, CD80 (B7-1 ) 、 CD86 (B7-2 ). This experiment has shown that DCs pulsed with TAA extracts of C57B/6J cells could stimulate effectively the responsiveness of syngenic splenic T cells to induce specific CTL against C57BL/6J cells.
RESUMO
Objective:The present study was designed to investigate whether transfecting DC with tumor derived total RNA is an effective way to induce CTL and antitumor immunity.Methods:DC were propagated from bone marrow(BM) of C57BL/6J(H 2k b?I A b)mice in vitro with GM CSF+IL 4.Tumor derived total RNA extracted from actively growing Hepa 1 6 cells was used to transfected DC.The phenotypes of DC were detected by FACS,the cytotoxicity of CTL was assayed by MTT method.Results:The tumor derived total RNA transfected DC exhibited much more and longer plasm membrane processes and increased expression of MHC Ⅰ?MHC Ⅱ?CD80(B7 1)?CD86(B7 2).Conclusion:This experiment has shown that DC transfected with tumor derived total RNA of C57BL/6J cells could stimulate effectively the responsiveness of syngenic splenic T cells to induce specific CTL against C57BL/6J cells.