Identification of High-affinity Novel Targeted Peptides for the Treatment of Human Ovarian Cancer

Background: To investigate the potential of the phage display-identified tumor cellbinding peptide as a biomarker of epithelial ovarian cancer using phage display technology. Method: The Ph.D.-7 Phage Display Peptide Library was used to identify the specific conjugated phages with SKOV3 epithelial ovarian cancer cells, while Chinese hamster ovary cells formed the basis. After employing the rapid differential screening method invitro, the enzyme-linked immunosorbent assay (ELISA), DNA sequencing, immunohistochemistry, immunofluorescence, and the competitive inhibition test of synthetic peptides were used to determine the affinity and specificity of the phages with SKOV3 cells. Results: Using bio panning, we screened the phages, showing a 3590-fold increase after the third round. A total of 61 titers of the phage were randomly selected for ELISA and 10 kinds of the phages with an optical density >0.5 were used for DNA sequencing. Clones of the phage TRRNIPN were derived from DNA sequencing based on ELISA, exhibiting both the brown granular phenomenon and green fluorescence. The specific targeted peptide TRRNIPN was incorporated in tumor cells through the competitive inhibition test. Conclusion: The results of our study indicate that the phage display identified polypeptide TRRNIPN may be an effective biomarker for the early diagnosis and targeted therapy of ovarian cancer

Comparative study of laparoscopic surgery combined with chemotherapy drugs in patients with cervical cancer / 中国生化药物杂志

Objective To investigate the clinical efficacy of laparoscopic surgery combined with chemotherapy drugs in patients with cervical cancer. Methods 60 patients with cervical cancer treated in our hospital from January 2015 to July 2016 were randomly divided into two groups:control group (n=30) and observation group (n=30). The two groups were treated with chemotherapy, the control group was treated with open surgery, the observation group was treated with laparoscopic surgery, the clinical efficacy of the two groups were compared. Results The operation time of the observation group was longer than that of the control group (P<0.05). The amount of bleeding, the exhaust time and the length of stay in the observation group were shorter than those in the control group (P<0.05). Conclusion Laparoscopic surgery for cervical cancer patients is effective, less trauma, and is conducive to postoperative recovery. It is worthy of popularization and application.

Effect of labetalol in treatment of severe preeclampsia / 中国生化药物杂志

Objective To investigate the effect of labetalol in treatment of severe preeclampsia clinical effect.Methods The control group of severe preeclampsia patients received routine clinical treatment,the study group were treated with labetalol,two groups of patients with severe preeclampsia were treated for 7 d.Results After treatment,the two groups of DBP,SBP,HR,and 24 HUP were significantly lower than before,the study group improved the above indicators better(P<0.05).The study group of patients with severe preeclampsia premature delivery rate,postpartum hemorrhage rate,neonatal asphyxia rate(10.20%,8.16%,6.12%)were significantly lower than the control group(28.57%,34.69%,20.41%)(P<0.05).Conclusion The application of conventional therapy combined with labetalol can significantly improve the hypotensive effect of severe preeclampsia,is conducive to the protection of maternal physical and mental health and life safety.

The effect of COH and IVM on mitochondrial membrane potentials and cytoskeleton / 实用医学杂志

Objective To investigate the effect of different maturation methods on mitochondrial functions of oocytes and the possible mechanism. To explore novel ideas for developing assisted reproductive technology (ART). Methods Female mice were used as models and randomly allocated into three groups, COH, IVM and NC control. Oocytes maturated with different methods which were all simulated with those treatments in human IVF cycle. Immunofluorescence were used to measure the mitochondrial membrane potentials and analyze the cy-toskeleton. Results The mitochondrial membrane potential in the COH group was significantly lower than that in NC group and IVM group (P < 0.05). The proportion of normal cytoskeleton including spindle structure and chromosome configuration in the COH group and IVM group were significantly lower than that in the NC group (PCOH < 0.01, PIVM < 0.05). Conclusions Both COH and IVM can affect mitochondrial functions.

Effect of magnesium sulfate combined with calcium channel blockers on cystatin C,uric acid, alpha fetal protein and urinary transferrin levels in patients with hypertension pregnancy / 中国生化药物杂志

Objective To explore the effect of magnesium sulfate combined with calcium channel blockers on cystatin C, uric acid, alpha fetal protein and urinary transferrin in patients with hypertension pregnancy.Methods 75 cases with hypertension of pregnancy were selected and divided into two groups, control group(n=37) were treated with conventional therapy, experiment group(n=38) were treated with magnesium sulfate combined with calcium channel blockers on the basis of control group.Cystatin C, uric acid, alpha fetal protein and urinary transferrin were compared after treatment between two groups.Results Compared with control group, Cys C, uric acid, serum AFP l, urinary TRF levels were all lower in experiment group( P<0.05), serum hematocrit, whole blood viscosity and erythrocyte aggregation index were all lower in experiment group(P<0.05).The total efficiency of experiment group was significantly higher than control group (P<0.05).Conclusion Magnesium sulfate combined with calcium channel antagonists in treatment of hypertensive disorder complicating pregnancy patients has good clinical curative effect, can effectively improve blood viscosity, reduce serum Cys C, UA, AFP and urinary TRF level.

CYP19 gene variant confers susceptibility to endometriosis-associated infertility in Chinese women

An aromatase encoded by the CYP19 gene catalyzes the final step in the biosynthesis of estrogens, which is related to endometriosis development. To assess the association of CYP19 gene polymorphisms with the risks of endometriosis, chocolate cysts and endometriosis-related infertility, a case-control study was conducted in Chinese Han women by recruiting 225 healthy control females, 146 patients with endometriosis, 94 endometriosis women with chocolate cyst and 65 women with infertility resulting from endometriosis, as diagnosed by both pathological and laparoscopic findings. Individual genotypes at rs2236722:T>C, rs700518:A>G, rs10046:T>C and [TTTA]n polymorphisms were identified. Allelic and genotypic frequencies were compared between the control group and case groups by chi-square analysis. Odds ratios (ORs) and 95% confidence intervals (95% CIs) were determined by logistic regression analysis to predict the association of CYP19 gene polymorphisms with the risk of endometriosis, the related chocolate cysts and infertility. The genotype distributions of the tested CYP19 gene polymorphisms were not significantly different between the healthy control group and the endometriosis/endometriosis with the chocolate cyst group. However, the CYP19 rs700518AA genotype was significantly associated with an increased risk of endometriosis-related infertility (55.4% in the infertility group vs 25.3% in the control group, P<0.001; OR (95% CI): 3.66 (2.06-6.50)) under the recessive form of the A allele. Therefore, we concluded that in Chinese Han females CYP19 gene polymorphisms are not associated with susceptibility to endometriosis or chocolate cysts, whereas CYP19 rs700518AA genotype confers genetic susceptibility to endometriosis-related infertility.

Expression of nuclear factor kappaB, MCP-1 and RANTES concentration in peritoneal fluid in women with endometriosis / 实用医学杂志

Objective To compare the concentration of nuclear factor-kappa B (NF-κB), MCP-1 and RANTES in peritoneal fluid in women with endometriosis and normal peritonal fluid ,To explore the relationship between these cytokines with pathogenesis of endometriosis. Methods There were 2 groups:the endometriosis group and the control group.Double-antibody sandwich ABC-ELISA technique was adopted for determinating concentration of NF-кB、MCP-1 and RANTES in peritoneal fluid. Results Peritoneal fluid level of NF-κB MCP-1 and RANTES in endometriosis patients was higher than the control group (P < 0.05). NF-κB and MCP-1, NF-κB and RANTES expressed positive correlation by linear correlation analysis in both EMs group and control group , correlation coefficients were 0.385, 0.569(EMs group), 0.474, 0.388(P < 0.05). Conclusions NF-кB、MCP-1 and RANTES are mutual affection and promote endometriosis in the pathogenesis.

Prevention and treatment of iatrogenic ureter and bladder injury / 解放军医学杂志

Objective To investigate the etiological factors and measures for prevention and treatment of iatrogenic ureteral and bladder injury during obstetric and gynecologic operations.Methods Iatrogenic ureteral and bladder injury happened in 31 patients,who underwent traditional gynecologic operation from June 1996 to May 2006 at the Department of Gynecology and Obstetrics of the Second Affiliated Hospital of Wenzhou Medical College.The etiological factors and treatment for iatrogenic ureteral and bladder injury were analyzed retrospectively.Results The median age of the patients was 44 years(ranged from 27 to 61years).2 cases of iatrogenic ureteral injury and 3 cases of iatrogenic bladder injury occurred in hysterectomy.3 cases of iatrogenic ureteral injury and 2 cases of iatrogenic bladder injure occurred in subtotal hysterectomy for precancerous lesion.5 cases of iatrogenic ureteral injury and 7 cases of iatrogenic bladder injury occurred in radical hysterectomy.One case of iatrogenic ureteral injury and 8 cases of iatrogenic bladder injury occurred in cesarean section.Intraoperative ureteral injury in 7 patients was repaired by end-to-end anastomosis,and intraoperative bladder injury in 15 patients were repaired during operation.In 4 patients the ureteral injury was found after operation,including 2 cases of ureterovaginal fistula,and the injury was repaired after resection of fistulas.Bladder injury was found in 5 cases after operation,and the injuries were repaired by closure of fistulas of bladder 3 months later.All cases recovered with no relapse during the follow-up period of 5 months to 10 years.Conclusions Iatrogenic injury occurs during gynecological surgery should be prevented first.Early discovery and effective treatments can achieve good therapeutic effects.