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Chinese materia medica has a wide range of clinical applications， but it has many active ingredients with different physicochemical properties， and the target organs， action pathways and mechanisms for different ingredients to exert their efficacy are not the same. Therefore， it is difficult to design and develop a co-delivery system loading multiple components of Chinese materia medica to maximize the synergistic therapeutic efficiency. Based on the characteristics of effectiveness and functionality of active ingredients， the strategies for multi-component co-delivery of Chinese materia medica can be categorized into two types：firstly， based on the effectiveness of active ingredients， new carriers such as liposomes， nanoparticles can be constructed to load multi-components of Chinese materia medica. secondly， based on the functionality of some active ingredients of Chinese materia medica， they are employed in the construction of co-delivery system， which can give play to the dual characteristics of their own efficacy and preparation functions. In this paper， we summarized the relevant research progress of the above two types of multi-component co-delivery strategies， and mainly discussed the pharmaceutical functions of the active ingredients in co-delivery systems， in order to find a more suitable multi-component co-delivery strategy， promoting the design and development of new delivery systems of Chinese materia medica.
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Objective@#To systematically evaluate the effectiveness of high intensity interval training (HIIT) on body weight and body composition of overweight and obese female college students. In order to provide a theoretical basis for choosing HIIT method.@*Methods@#Randomized controlled trials (RCTs) published till December 14, 2020 were searched in PubMed, the Cochrane Library, Web of Science, Embase, CNKI, CBM, VIP and WanFang Databases. Literature screening was conducted based on inclusion and exclusion criteria, methodological quality evaluation, Meta analysis and publication bias test were conducted on the included literature.@*Results@#There were 20 RCTs, among which 14 studies graded as moderate quality. Meta analysis showed that HIIT could significantly reduce the weight of overweight and obese female college students ( MD =-4.22, 95% CI =-7.20--1.25, P < 0.01 ). Improved body fat rate ( MD =-5.31, 95% CI =-6.88--3.73, P <0.01), BMI ( MD =-2.11, 95% CI =-2.65--1.56, P < 0.01 ), total body fat ( MD =-3.66, 95% CI =-4.89--2.43, P <0.01), abdominal fat ( MD =-0.31, 95% CI =-0.47--0.15, P < 0.01 ), trunk fat ( MD =-2.15, 95% CI =-2.86--1.44, P <0.01) were observed. There was no significant effect on lean body weight ( MD =0.42, 95% CI =-0.94-1.78, P =0.55).@*Conclusion@#HIIT can significantly reduce the weight and improve body composition in overweight and obese female college students. It can provide a reliable basis for long term HIIT in overweight and obese female college students.
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Integrated medical curriculums are becoming more and more popular in medical colleges at home and abroad. In this article, the methods and teaching of integrated digestive system course at Shanghai Jiao Tong University School of Medicine was thoroughly introduced from the theoretical course, PBL course, trainee course, assessment method and effect evaluation. The successful experience of practice of the integrated digestive system curriculum was summarized as follow: strong guarantee from systems, adequate reserves of teaching faculty and good mechanism of feedback. Also, the problems in the practice of the curriculum were presented, which include the shortage of corresponding textbook and of deep running-in between teachers.
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Background:Deoxyhypusine synthase(DHPS)is a key factor in post-translational modification of the precursor of eukaryotic initiation factor 5A(eIF-5A),and eIF-5A is closely related to the regulation of proliferation and invasion of tumor cells. Aims:To investigate the expression of DHPS in gastric cancer and its clinical significance,and to explore the possible mechanism of its effect on metastasis of gastric cancer. Methods:Tissue microarray containing 92 gastric cancer tissues and paired adjacent cancerous tissues was employed to detect the DHPS expression by using immunohistochemical staining,and the correlation of DHPS expression with the clinicopathological characteristics of gastric cancer was analyzed. DHPS-siRNA and GC7,an inhibitor of DHPS were used,respectively to intervene human gastric cancer cell line MGC803. The invasive ability of MGC803 cells was assessed by cell invasion assay,and the expressions of metastasis-related proteins including vascular endothelial growth factor(VEGF),matrix metalloproteinase 2(MMP2)and MMP9 were detected by ELISA. Results:In 62(67.4%)cases of gastric cancer,DHPS was highly expressed,and its expression was closely related to tumor diameter,TNM stage and depth of invasion(P <0.05),but not related to gender,age,lymph node metastasis and distant metastasis(P >0. 05). Both DHPS-siRNA and GC7 could down-regulate the invasiveness of MGC803 cells,while the former could also reduce the expressions of VEGF,MMP2 and MMP9 proteins(P <0.05). Conclusions:DHPS is highly expressed in gastric cancer and associated with tumor invasion and progression. DHPS is expected to be a new target for diagnosis and treatment of gastric cancer because of its regulatory effect on invasion and metastasis of tumor cells.
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5#. The hybridoma cell lines grew well after continuous culture for more than three months or after being stored in liquid nitrogen for six months, and the titers of secreting McAbs were stable.Conclusion:The McAbs against respiratory syncytial virus are obtained. It lay a foundation for early diagnosis and further study of RSV.