Study on prescription screening and preparation process of compound danshen pulse capsule / 中国中药杂志

To develop gastric floating erodible plug pulse capsules with compound Danshen as the model drug, in order to realize the pulse release of traditional Chinese medicines. Through the study on impermeable capsules, optimized prescriptions, drug-containing rapid-release tablets and prescription screening, and erodible plug prescription and process, we successfully prepared compounded Danshen pulse capsule, so as to provide a new dosage form for controlling and treating heart disease to better cater to clinical demands.

In vivo and in vitro correlation of quality control components in Shuanghuanglian Oral Liquid / 中草药

Objective: To establish the in vitro and in vivo HPLC fingerprint of Shuanghuanglian Oral Liquid (SOL). Methods: The chromatographic fingerprint was obtained with Welchrom C18 column (150 mm × 4.6 mm, 5 μm); The mobile phase was methanol-0.2% phosphoric acid aqueous solution, with gradient elution and segmentation variable wavelength determination; The column temperature was maintained at 30°C; The injection volume was 10 μL. Results: Among 10 batches of samples prepared by single and different preparation methods, the overall similarity evaluation was taken using the Chromatographic Fingerprint Similarity Software for Chinese Materia Medica (Version 2004A), and the average similarities were all above 0.93. The better in vivo and in vitro correlation of the index constituents in SOL prepared by the method of Pharmacopoeia of People's Republic of China was obtained. Conclusion: The method had good precision, stability, and repeatability. The standard chromatographic fingerprint established has a better representativity, and the method provides the new ideas for establishing the new evaluation system of Chinese materia medica oral preparation.

Analysis of hantaviruses origin and molecular evolution in China / 第二军医大学学报

Objective: To investigate the first appearance and geographic distribution of hantaviruses in China. Methods: Some sequences of M segment of hantaviruses isolated in China were retrieved from GenBank. Some recently sequenced viruses which have not been registered in GenBank were obtained from authors who reported them. Program MEGA 3.1 was employed for the phylogenetic analysis of the above virus sequences. Nucleotide divergence rates were estimated by comparing the closely related sister sequences isolated in the same geographical region at no less than a 5-year interval. Based on these calculated divergence rates, together with their isolating time and region, we explored the first appearance and geographic distribution of hantaviruses in China. Results and conclusion: The distribution of hantaviruses was in cluster, with Guizhou Province having the most subgenotypes, which supports the previous hypothesis that hantaviruses first appeared in Guizhou Province of China. A novel subgenotype virus was found in Anhui Province, indicating that it might be easier for the virus to have mutation in Anhui Province. We also found that Gou3 strain might be the earliest one to separate from a common ancestral strain; it is speculated that hantaviruses migrated in China about 315 years ago.

Correlation between immune response to hepatitis B vaccine and HLA DRB1 * 07 DRB1 * 04 DRB1 * 1001, DQB1 * 0401 genes in Han population in Ningbo / 第二军医大学学报

Objective: To study the correlation between responses to Hepatitis B and HLA DRB1 * 07 DRB1 * 04 DRB1 * 1001, DQB1 * 0401 genes in Han population in Ningbo. Methods: A total of 240 Han people living in Ningbo received the routine vaccination of recombinant hepatitis B vaccine. The serum levels of anti-HBs antibody were examined and the subjects were divided into 2 groups according to the results: negative responses group (n = 120, anti-HBs D< 0.1) and positive response group (n = 120, anti-HBs D≥0.1). The distribution and frequency of DRB1 * 07, DRB1 * 04, DRB1 * 1001, DQB1 * 0401 genes alleles were determined with PCR using sequence-specific primers (PCR-SSP). Results: The sex and age were compatible between the 2 groups. The frequency of DRB1 * 07 in negative response group was significantly higher than that in positive response group (20.3%, vs 10.3%, P<0.05); the failure of vaccination was correlated with DRB1 * 07 (OR=0.45, P<0.05). The frequencies of HLA- DRB1 * 04 DRB1 * 1001, DQB1 * 0401 showed no obvious difference between the 2 groups. Conclusion: Failure or deficiency response to HBV vaccine in the Han people in Ningbo is associated with the HLA-DR antigen; HLA-DRB1 * 07 might be a susceptible gene for failure of HBV vaccination.

Screening for molecular markers for early diagnosis of lung cancer by bacteriphage display / 第二军医大学学报

Objective: To establish a lung cancer phage-peptide library and to screen for biomarkers for early detection of lung cancer. Methods: A phage display library was constructed using 30 lung-cancer tissue samples from Changhai Hospital. Protein-A/G agarose was used to enrich IgG from control sera as well as lung cancer sera. Five biopannings were carried out for enrichment of lung cancer-specific phage clones. Five hundred phage clones were randomly selected,those with D>2.0(lung cancer plasma pool v. s. control plasma pool) were selected for DNA sequencing and protein prediction. Results: (1) The recombination rate of the phage library was 60%,with the average phage titer being 3.0 × 106 pfu and a volume being 9.0 × 106 pfu. (2) Of 19 phage colonies selected by ELISA and sequenced,9 were cancer-related genes,8 with unknown function, and 2 were not related to cancer. Conclusion: The screened genes in the phage colonies might serve as biomarkers for the early diagnosis of lung cancer.

Correlation between immune response to hepatitis B vaccine and HLA DRB1*07 DRB1*04 DRB1*1001.DQB1*0401 genes in Han population in Ningbo / 第二军医大学学报

Objective:To study the correlation between responses to Hepatitis B and HLA DRB1*07 DRB1*04 DRB1*1001. DQB1*0401 genes in Han population in Ningbo.Methods:A total of 240 Han people living in Ningbo received the routine vaccination of recombinant hepatitis B vaccine.The serum levels of anti-HBs antibody were examined and the subjects were divided into 2 groups according to tile results:negative responses group(n=120.anti-HBs D

Microsatellite instability and loss of heterozygosity on chromosome 3p,9p and 14q in renal cell carcinoma / 第二军医大学学报

Objective:To investigate frequencies of microsatellite instability(MSI)and loss of heterozygosity(LOH)in renal ceil carcinoma(RCC),and to discuss the relationship of clinicopathological characteristics of RCC with MSI and LOH. Methods:Twelve microsatellite markers located at chromosomes 3p,9p and 14q were selected to investigate microsatellite alterations(MSI and LOH)in 31 RCC specimens and their paired metastasis specimens by polymerase chain reaction- polyacrylamide gel elect rophoresis-ethylene dibromide(PCR-PAGE-EB)staining and sequencing.Results:The frequency of MSI could reached 61.3% and that of LOH could reach 54.8%.The highest frequency of MSI was at locus of D9S168(32.3%);the highest frequency of LOH was at locus of D3S1289(21.4%).No correlation was found between MSI or LOH and the patients' age,sex,pathology type and metastastis,except that MSI was correlated with TNM stage of RCC(P