The stability study of Coptidis Rhizoma and Huanglian Jiedu Decoction / 中国药学杂志

RESULTS: Twenty-eight days later, the content of berberine hydrochloride in Coptidis Rhizoma Decoction in normal pressure Chinese materia Medica extractor group is from 0.066 9 to 0.065 5 mg��mL-1 and that in traditional decoction group is from 0.063 5 to 0.064 4 mg��mL-1. In Huanglian Jiedu Decoction (normal pressure Chinese materia medica extractor group and traditional decoction group), the content of jasminoidin is from 0.296 6 to 0.285 7 mg��mL-1 and from 0.275 0 to 0.261 2 mg��mL-1, baicalin decreased from 0.275 7 to 0.199 0 mg��mL-1 and from 0.219 4 to 0.162 4 mg��mL-1, berberine hydrochloride decreased from 0.026 2 to 0.005 0 mg��mL-1 and from 0.014 0 to 0.005 8 mg��mL-1, respectively. The result of pH determination of Coptidis Rhizoma and Huanglian Jiedu Decoction showed there is no difference during 1 month.

Primary neuroendocrine tumor of the testis: clinicopathological study of 7 cases / 中华男科学杂志

<p><b>OBJECTIVE</b>To investigate the clinicopathologic characteristics, diagnosis, differential diagnosis and treatment of primary neuroendocrine tumor (NET) of the testis.</p><p><b>METHODS</b>Using light microscopy and immunohistochemistry, we studied 7 cases of primary NET of the testis, reviewed relevant literature, and analyzed the clinical manifestations, histomorphologic and immunohistochemical characteristics, treatment and prognosis of the tumor.</p><p><b>RESULTS</b>The 7 male patients, at the mean age of 40.6 years, all presented with testicular painless masses, none accompanied with carcinoid syndrome. Histologically, the uniform tumor cells were arranged in trabecular, island, solid and/or flake structures and locally in a tubulo glandular pattern, round and polygonal in shape, with a small amount of lipid vacuoles in the eosinophilic cytoplasm. The cells had round nuclei with fine chromatin and rarely identified mitosis. Immunohistochemical staining showed that the tumor cells were positive for Syn, CgA, NSE and CK, with a Ki-67 positive rate of < 2%.</p><p><b>CONCLUSION</b>Primary NET of the testis is a rare and low-grade malignancy. Early diagnosis and surgical resection are essential for good prognosis. Immunohistochemistry helps its diagnosis and differential diagnosis from other metastatic neuroendocrine carcinoma, teratomas with carcinoid, seminoma, and Sertoli cell tumor.</p>

Preliminary study on the MLVA genotyping of Mycobacterium tuberculosis isolates in Fujian province / 中华流行病学杂志

Objective To preliminarily understand the genotyping characteristics regarding the variable number tandem repeats (VNTR) of Mycobacterium tuberculosis clinical isolates so as to provide evidence for the development of tuberculosis control and prevention programs in Fujian province.Methods Fifteen VNTR locus sets were used to detect the clinical isolates from the fifth surveillance project on tuberculosis resistance,in Fujian province.BioNumerics version 4.5 were used to analyze the cluster from the results generated by genotyping.Results 313 Mycobacterium tuberculosis isolates were divided into 9 clusters,including Ⅰ,Ⅱ,Ⅲ,Ⅳ,Ⅴ,Ⅵ,Ⅶ,Ⅷ and Ⅸ,with the number of 220,9,48,2,1,3,10,10,10 isolates,respectively.Cluster Ⅰ was the major lineage,accounting for 70.3％ (220/313) of the total.Resistance rates of cluster Ⅰ isolates to isoniazid,streptomycin,ethambutol and multi-drug-resistaut were not statistically different from other clusters (P＞0.05).However,resistance rate to rifampicin (RFP) was significantly higher than that of other isolates of the clusters,33.2％ (73/220) vs.20.4％ (19/93) (P＜0.05).Conclusion The strains isolated from Fujian province showed significant polymorphism on genotyping.Cluster Ⅰ seemed to be the dominant,calling for the close monitoring program on cluster Ⅰ strains.Results from our initial studies demonstrated the existence of significant correlation between cluster Ⅰ strains and drug resistance to RFP.

Large cell calcifying Sertoli cell tumor of the testis: a clinicopathological observation / 中华男科学杂志

<p><b>OBJECTIVE</b>To investigate the clinicopathological characteristics of large cell calcifying Sertoli cell tumor (LCCSCT) of the testis.</p><p><b>METHODS</b>We studied a case of LCCSCT by light microscopy, Western blotting and immunohistochemistry, reviewed relevant literature, and analyzed the clinical, morphological and immunohistochemical features, treatment and prognosis of the tumor.</p><p><b>RESULTS</b>The patient was a 25 years old man. Pathohistologically, the tumor was characterized by a mass of polygonal tumor cells in a tubular and trabecular growth pattern, with abundant acidophilic cytoplasm, enlarged vesicular nuclei, and extensive calcified debris in stroma. The tumor cells were positive for inhibin, S-100, vimentin and alcian blue, but negative for PLAP, SMA, CK, AFP and periodic acid-Schiff (PAS) reaction.</p><p><b>CONCLUSION</b>LCCSCT is a rare testicular sex cord stromal tumor. Its diagnosis is based on immunohistochemical staining, and it is to be differentiated from other lesions of the testis, including seminoma, Leydig cell tumor, Sertoli cell node, and androgen insensitivity syndrome. For the treatment of LCCSCT, surgical resection often has a good prognosis.</p>

Correlation of hK6 expression with clinicopathological features and prognosis in epithelial ovarian cancer / 中华肿瘤杂志

<p><b>OBJECTIVE</b>To approach the relationship between the expression of hK6 in ovarian neoplasm and clinicopathological variables and prognosis in ovarian cancer patients for finding a new tumor marker of the ovarian cancer.</p><p><b>METHODS</b>The expression of hK6 was detected by immunohistochemistry in 19 cases of benign, 11 cases of borderline and 45 cases of malignant ovarian neoplasms and statistically analyzed whether its expression correlate with clinicopathological variables and prognosis in patients with ovarian cancer.</p><p><b>RESULTS</b>The expression of hK6 in ovarian cancer tissues (60.0%) was significantly higher than that in the benign (15.8%) and borderline (27.3%) ovarian neoplasm tissues (P < 0.01). The expression of hK6 in higher-grade ovarian cancer tissues (68.4% ) was higher than that in low-grade ones (14.3%, P < 0.05). The expression of hK6 in late-stage (stage III, 76.7%) was significantly higher than that in early-stage (stage I or II, 26.7%, P < 0.01). The expression of hK6 was significantly higher in patients with lymph node metastasis (77.8%) than that in patients without (33.3%, P < 0.01). The expression of hK6 in the cancer tissues in the patients died, or with reccurence or metastasis within 3 years after surgery was higher (75.0%) than that in the patients with stable disease (42.9%, P < 0.05).</p><p><b>CONCLUSION</b>The expression of hK6 in ovarian cancer was higher than that in benign and borderline ovarian neoplasms. The expression of hK6 is higher in the ovarian cancer of late stage, higher-grade, with lymph node metastasis and is associated with a poorer prognosis. hK6 may become a new markers in prediction of prognosis of the patients with ovarian tumors.</p>