Detection of BCR/ABL fusion gene by fluorescence in situ hybridization and its clinical application / 中国实验血液学杂志

This study was aimed to investigate the clinical value of detecting BCR/ABL fusion gene by fluorescence in situ hybridization (FISH). The conventional cytogenetic test and detection of BCR/ABL fusion gene by FISH for bone marrow of patients with newly diagnosed chronic myeloproliferative disease or myelodysplastic and myeloproliferative disorders, acute lymphocytic leukemia and chronic myelogenous leukemia (CML) after allogeneic hematopoietic stem cell transplantation were carried out. The results showed that (1) out of 46 newly diagnosed as chronic myeloproliferative disease or myelodysplastic and myeloproliferative disorders, 22 cases were diagnosed as CML, the FISH detection showed all positive (100%), while cytogenetic test showed 86.4% (19/22) positive, in the other 24 patients who were diagnosed as other chronic myeloproliferative disease or myelodysplastic and myeloproliferative disorders, BCR/ABL fusion gene all were be detected as negative 100% by FISH, while the cytogenetic test of bone marrow in 3 cases supported the diagnosis of CML, and the diagnosis of myelodysplastic disorder in 1 case; (2) in 3 out of 7 acute lymphocytic leukemia cases the BCR/ABL fusion gene could not be detected by FISH; (3) the BCR/ABL fusion gene could be detected by FISH in 2 cases of CML received allogeneic hematopoietic stem cell transplantation, with abnormal threshold 6.5% and 1.2% respectively. It is concluded that the detection of BCR/ABL fusion gene by FISH is sensitive and reliable, which is very important for the diagnosis and differential diagnosis of chronic myeloproliferative disorders, myelodysplastic and myeloproliferative disease, as well as definite diagnosis of Ph(+) acute lymphoblastic leukemia. This method also has an important significance for monitor of minimal residual disease in CML patients received allogeneic hematopoietic stem cell transplantation.

The influence of accelerated aging on color and microstructure of light-curing composite resin / 华西口腔医学杂志

<p><b>OBJECTIVE</b>The aim of this investigation was to examine the changes of the color stability, surface microstructure and chemical constitution of light-curing composite resin after accelerated aging, and the relations between them.</p><p><b>METHODS</b>Four light-curing composite resin were aged in an accelerated aging instrument. The color was measured by CIE L*a*b* with a spectrophotometer after treatment for 24 h and 96 h. And the color differences were calculated. Environmental scanning electron microscopy (ESEM) and Fourier transform infrared spectroscopy (FTIR) spectrometer were used to examine the microstructure and chemical composition of the specimens before and after accelerated aging. The color differences were analyzed statistically by repeated-measures two-way analysis of variance and t-test after aging for 24 h and 96 h. The level of significance was defined as alpha=0.05.</p><p><b>RESULTS</b>The materials demonstrated statistically significant differences in color after aging between the 24 h and 96 h (P<0.05). There were significant influences on the microstructure and the chemical composition after aging. The matrix appeared some concaves and pores, the filler particles exposed after aging. The energy of chemical bonds were weaken or broken under the aging, and the unsaturated polymer reacted again.</p><p><b>CONCLUSION</b>The color differences of the composite resin increase with the aging time and irradiation dose. The hybrid filled composites have the best color stability.</p>

Study on the matching of the shade between beverage and modifying porcelain shade guide / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To evaluate the matching of the shade between beverage and modifying porcelain shade guide according to Munsell color order system, thus to provide the reference basis for selecting modifying porcelain to mimic the stain of natural tooth by technician.</p><p><b>METHODS</b>The shade of Vita Akzent, Vita Interno, Shofu Vintage & Unibond and Noritake Super Porcelain EX-3 shade tabs as well as 15 kinds of beverage were measured according to Munsell color order system on Color-Eye 7000A spectrophotometer. The difference of the frequency of approximate hue, value, and chroma between shade tabs and beverage were compared by calculating the Fisher exact probabilities.</p><p><b>RESULTS</b>The frequency of approximate hue between 4 kinds of shade tabs and 15 kinds of bev-erage was significant different (P<0.05), while the frequency of approximate value, and chroma was not significant different (P>0.05).</p><p><b>CONCLUSION</b>Except the hue, the color distribution of 4 kinds of shade tabs was similar to that of 15 kinds of beverage. But the color of beverage also can be approximately matched by any kind of modifying porcelain by mixing porcelain powder of appropriate hue, value, and chroma.</p>

Engraftment of ex vivo expanded mononuclear cells from human umbilical cord blood in NOD/SCID mice / 中国实验血液学杂志

This study was purpose to explore the effect of combination of SCF, FL, TPO without serum and stroma on the ex vivo expansion of the mononuclear cells derived from umbilical cord blood (CB) and to evaluate the proper infused time and implantation of expanded cells. Mononuclear cells derived from CB were cultured with the combination of SCF, FL and TPO for 14 days. At 0, 7, 10 and 14 days, the total mononuclear cells in culture were counted, CD34+ cells and CD34+CXCR4+, CD34+CD49d+ cells were assayed by flow cytometry, and CFU were determined. Mononuclear cells from CB cultured for 7 days ex vivo in serum- and stroma-free medium containing FL+SCF+TPO were infused into sublethally irradiated NOD/SCID mice. Six weeks after transplantation, the human cells were assessed by flow cytometry and PCR. The results showed that after culturing ex vivo in the presence of SCF+FL+TPO for 14 days, the total number of nuclear cells significantly increased in the early stage of culture, CD34+, CD34+CD49d+ cells reached a peak level at 7 days and then decreased, while the peak level of CFU, CD34+CXCR4+ cells was at 10 days, then decreased. Human hematopoietic cells could be detected in the marrow of the recipients at 6 weeks after infusion and the rate of success of NOD/SCID mice implanted with the expanded cells was higher than mice transplanted with fresh sample and control (p<0.05). It is concluded that day 7-10 may be an optimal harvest time for transplantation. The expanded cells with FL+SCF+TPO can be engrafted into the bone marrow of sublethally irradiated NOD/SCID mice. The engrafting potential of cord blood may be enhanced via up-regulating CXCR4 and CD49d expression on the CD34+ cells.

Evaluation of bone marrow biopsy and smear examinations in differential diagnosis for patients with pancytopenia by simultaneously compartive study / 中国实验血液学杂志

To simultaneously compare and evaluate the examinations of bone marrow smear and trephine biopsy in differential diagnosis and therapeutical effect of pancytopenia patients, the differences between bone marrow smear and trephine biopsy in degree of cellularity, misdiagnosis rate and therapeutical effect for 71 patients with pancytopenia were analyzed. The results showed that the degree of cellularity in bone marrow biopsy for cytologic morphology was higher than that from the smear, but misdiagnosis rate in the biopsy was lower than that in the smear. In conclusion, bone marrow biopsy is necessary to the differential diagnosis and more valuable for evaluation of therapeutical effect and prognosis of pancytopenia patients.