Application of allogeneic amniotic membrane for myringoplasty in the treatment of traumatic perforation of tympanic membrane / 中国耳鼻咽喉头颈外科

OBJECTIVE To investigate the clinical efficacy of using allogeneic amniotic membrane for myringoplasty in the treatment of traumatic perforation of tympanic membrane.METHODS 27 cases of traumatic perforation of tympanic membrane underwent myringoplasty with allogeneic amniotic membrane between July 2003 and February 2008 were analyzed retrospectively.The perforation healing rate and hearing improvement were studied.RESULTS All cases were followed-up for more than 6 months. The perforation healing rate was 96.3%and PTA gain was(12?3.7) dB.CONCLUSION The virtue of myringoplasty with allogeneic amniotic membrane is the plenty of service,convenient operation,little damage and good histocompatibility and effect.

The construction of human Eotaxin and its prokaryotic expression vector / 医学研究生学报

Objective:To construct prokaryotic cell expression vector of human Eotaxin for expressing and examining its activity,and for constructing the N-terminal mutations of human Eotaxin.Methods: Extract the total RNA from peripheral monocyte,and Eotaxin gene coding region was obtained by RT-PCR.The fragment was sequenced and analysed after cloning into T vector,then clone to prokaryotic cell expression vector-PET30a~+ and expressed in BL21(DE?3).Results:The right cloning of human Eotaxin and its prokaryotic cell expression were obtained.Conclusion: The construction and prokaryotic cell expression of human Eotaxin lay a foundation for its more expressing and examining their activity,and for constructing the N-terminal mutations of human Eotaxin.

Construction and expression of N-terminal mutants of human Eotaxin / 第三军医大学学报

Objective To construct prokaryotic cell expression vector of human Eotaxin mutants.Methods By point mutation,eight amino acid residues in the N-terminal(residues of 3-7)and N-loop(residue 14)regions of Eotaxin were individually mutated to methionine and residue 14 was delleted or methiomine was inserted after the residue 14,and then cloned respectively into prokaryotic cell expression vector-PET30a+.Results Eight N-terminal and N-loop mutants of human Eotaxin and their prokaryotic cell expression vector-PET30a+ were gained.Conclusion The successful construction of prokaryotic cell expression vector of human Eotaxin mutants lays a foundation for their expression and biological activity and for filtering antagonists of CCR3.