Role of serum Golgi protein 73 in nonalcoholic fatty liver disease / 临床肝胆病杂志

Golgi protein 73 (GP73) is a Golgi transmembrane protein that can be released into blood by cleavage. An increasing number of studies have shown that the increase in serum GP73 is closely associated with the development and progression of nonalcoholic fatty liver disease (NAFLD) and serum GP73 is expected to be used as a potential serological marker for the diagnosis and assessment of NAFLD. This article reviews the research advances in serum GP73 in NAFLD and analyzes its potential mechanism of action, so as to provide new options for the therapeutic targets of NAFLD.

Role of bone morphogenetic protein-4 in the pathogenesis of nonalcoholic fatty liver disease / 临床肝胆病杂志

Nonalcoholic fatty liver disease (NAFLD) has become one of the most common chronic liver diseases in the world, and it seriously harms human health. Recent studies have found that bone morphogenetic protein 4 (BMP4) might be associated with NAFLD. This article reviews the latest advances in the research on the association between BMP4 and NAFLD in China and globally and explores the potential mechanism of action of BMP4 on NAFLD, in order to provide new ideas for the prevention and treatment of NAFLD.

Role of angiopoietin-like proteins in the development of nonalcoholic fatty liver disease / 临床肝胆病杂志

Angiopoietin-like proteins (ANGPTLs) are a family of secretory glycoproteins recently found to be constitutionally homologous with angiogenin and play a role in the regulation of angiogenesis. In recent years, more and more studies have shown that ANGPTLs play an important role in glucose and lipid metabolism, inflammation, and tumor. As we all know, nonalcoholic fatty liver disease and its disease spectrum are closely associated with metabolism, inflammation, and tumor. This article reviews the role of ANGPTLs in various diseases associated with nonalcoholic fatty liver disease, in order to provide new ideas for the prevention and treatment of nonalcoholic fatty liver disease in clinical practice.

Research advances in autophagy-related signal molecules in nonalcoholic fatty liver disease / 临床肝胆病杂志

Nonalcoholic fatty liver disease (NAFLD) has become one of the main causes of chronic liver diseases worldwide and is closely associated with metabolic syndrome. In-depth studies on the pathogenesis of NAFLD in recent years have shown that autophagy is a highly conservative process in eukaryotes and plays an important role in the progression of NAFLD, and therefore, it is expected to become a new target for the treatment of NAFLD. This article reviews the research advances in autophagy-related signal molecules in NAFLD, in order to provide new ideas for the treatment of NAFLD.

Research progress of linezolid-induced lactic acidosis / 中国临床药理学与治疗学

Linezolid is an oxazolidinone antibacterial agent used in infections caused by gram-positive cocci such as methicillin-resistant staphylococcus aureus, penicillin-resistant pneumococcus and vancomycin-resistant enterococcus. Lactic acidosis is one of the adverse reactions of linezolid. The risk factors of lactic acidosis caused by linezolid are long-term exposure, liver dysfunction, renal dysfunction, mitochondrial DNA A2706G polymorphism, combined use of drugs affecting mitochondrial function, etc. The symptoms of lactic acidosis caused by linezolid are nausea, vomiting, drowsiness, shortness of breath, tachycardia, and hypotension, etc., which can be identified early by close monitoring of laboratory indicators such as blood lactic acid, pH, and blood drug concentration. The mechanism of lactic acidosis induced by linezolid may be related to mitochondrial toxicity. The lactic acidosis of linezolid can be caused by reducing drug dose, stopping drug or even in vitro renal replacement therapy, and strengthening symptomatic support therapy if necessary. This review is intended to provide ideas for the clinical prevention and treatment of lactate acidosis caused by linezolid.

The role and mechanism of myeloid-derived suppressor cells in mice with nonalcoholic steatohepatitis / 中国医师杂志

Objective To investigate the effects of myeloid-derived suppressor cells (MDSCs) on nonalcoholic steatohepatitis mice.Methods (1) Male C57BL/6 mice aged 6-8 weeks were randomly divided into normal diet group,CDAA group(choline deficient amino acid diet) and CSAA group (choline sufficient amino acid diet).(2) After the success of the non-alcoholic steatohepatitis model,serum was collected from some mice to detect biochemical indexes;Liver tissue was retained for microscopic observation by hematoxylin-eosin (HE) staining;The changes of T cell subsets in peripheral blood of mice in each group were detected by flow cytometry.In addition,The proportion and subtype of CD1 lb + Gr-1 + MDSCs cells in liver,spleen,blood and bone marrow were also detected by flow cytometry.(3)The bone marrow-derived Gr-1highLy-6G+ was purified by magnetic bead sorting technique,and the purified Gr-1highLy-6G+ was transferred into non-alcoholic steatohepatitis (NASH) mice by tail vein injection.The role of MDSCs in NASH was analyzed by detection of serological indexes of liver function and pathological dyeing.Results (1) There was no significant difference in body weight and liver index between the groups (P ＞ 0.05).Serological indicators:alanine aminotransferase (ALT),aspartate aminotransferase (AST),blood glucose,interleukin (IL)-6 and interferon-γ (INF-γ) in the model group were significantly higher than those in the normal group (P ＜ 0.01);microscopic findings:the liver in CDAA,CSAA group showed varying degrees of steatosis;the steatosis in CDAA group was much more severe.(2) Flow cytometry showed that ① the percentage of CD4 + CD8-T and CD4-CD8 + T decreased and CD4/CD8 ratio decreased in CDAA group;② the bone marrow MDSCs of CDAA group is lower than that of normal group (P ＜ 0.05);the MDSCs of peripheral blood in CDAA group is lower than that of normal group (P ＞ 0.05);the MDSCs of liver in CDAA group is lower than that of normal group (P ＜ 0.01).③ subgroup comparison,bone marrow,liver CD11 b + Gr-1 high Ly-6G + (G-MDSC):CDAA group ＞ normal group (P ＜ 0.0l),CD11 b + Gr-1 dim Ly-6G-(M-MDSC) showed a downward trend,CDAA group ＜ normal group;(3) Serum AST and ALT levels of NASH mice who receiving Gr-1highLy-6G+ MDSCs from normal bone marrow were significantly decreased (P ＜ 0.001),and histopathological changes were alleviated.Conclusions (1) The NASA mouse model can be successfully established on the CDAA diet.(2) The CDAA-induced NASH mice may have immune dysfunction,mainly manifesting in the change of bone marrow MDSCs subpopulations and the increase of CDllb + Gr-1highLy-6G+ (G-MDSC).(3) MDSCs derived from normal mouse bone marrow can alleviate the pathological changes of NASH.

Research advances in the role of myeloid-derived suppressor cells in liver diseases / 临床肝胆病杂志

In recent years, more and more studies have shown that myeloid-derived suppressor cells (MDSCs) participate in the development and progression of various chronic liver diseases including chronic viral hepatitis, alcoholic liver disease, nonalcoholic fatty liver disease, autoimmune liver diseases, and liver cancer. As a type of cells derived from bone marrow progenitor cells and immature myeloid cells, MDSCs play an important role in the development, progression, and repair of liver diseases by regulating inflammatory response and the differentiation and function of immune cells. This article reviews the research advances in the association between MDSCs and various liver diseases, in order to provide new thoughts for the clinical diagnosis, prognosis, and treatment of chronic liver diseases.

Correlation analysis between sleep beliefs and attitudes and sleep quality at home in patients with stable coronary heart disease / 中国实用护理杂志

Objective@#To investigate the relationship between sleep beliefs and attitudes and sleep quality in patients with stable coronary heart disease.@*Methods@#Totally 390 patients with stable coronary heart disease were investigated by the Pittsburgh Sleep Quality Index (PSQI), Dysfunctional Beliefs and Attitudes about Sleep Scale-16(DBAS-16) and a general information questionnaire which was designed by researchers.@*Results@#48.2% of patients with stable coronary heart disease reported poor sleep quality at home, and the total score of PSQI was (7.99 ± 4.061), the total score of DBAS was (47.44 ± 9.77). Univariate anova showed that age (F=14.567, P<0.01), gender (H=16.657, P<0.01), marital status (F=4.706, P=0.031), job status (F=15.738, P<0.01) and the number of comorbid diseases (F=4.674, P=0.003),are factors affecting sleep quality. Pearson correlation analysis showed that there were significantly negative correlation between PSQI and DBAS (r=-0.260, P<0.01). Multiple Linear Regression showed that sleep beliefs and attitudes, gender, age, the number of comorbid diseases were influencing factors of sleep quality (R2=0.247, F=17.907, P<0.01).@*Conclusions@#The sleep quality at home in patients with stable coronary heart disease was closely related to sleep beliefs and attitudes. Nurses should pay more attention to the sleep quality of patients, correct their incorrect sleep cognition timely, and improve their sleep quality.

Investigation on the utilization of parenteral nutrition preparations in 12 Hospitals of Jiangsu / 中华临床营养杂志

Objective To investigate the use of parenteral nutrition preparations in Jiangsu Province,and to provide reference for the standardized management of parenteral nutrition preparations.Methods 720 cases using parenteral nutrition preparations from January 2017 to June 2017 in the department of general surgery of 12 hospitals in Jiangsu province were selected.The rate of nutritional risk screening,the indications of parenteral nutrition,the way of infusion,the rationality and economy of the prescriptions were retrospectively evaluated.The calorie,amino acid content,non-protein calorie/nitrogen ratio,glycolipid ratio and cation concentration of the patients received total parenteral nutrition were calculated.Results The total costs of parenteral nutrition preparations of 720 cases were 1.614 1 millions,and 346 cases did not have the indications for parenteral nutrition.The results of prescription comment showed that only 16 patients were screened for nutritional risk by Nutritional Risk Screening 2002 tool at admission.544 cases were intravenous dripped with amino acid and fat emulsion from peripheral vein.In the 176 total parenteral nutrition prescriptions,there were 39 non-protein calorie/nitrogen ratio cases,15 glycolipid ratio cases,69 cation concentration cases,61 calorie cases and 32 amino acid content cases failing to comply with the recommendation of the guidelines.Only 31 total parenteral nutrition prescriptions were completely reasonable.Conclusion The costs of parenteral nutrition preparations used in hospitals of Jiangsu are high but the rate of rationality is low.Nutrition support team should be established to regulate the use of parenteral nutrition preparations and save medical resources.

Correlation analysis between sleep beliefs and attitudes and sleep quality at home in patients with stable coronary heart disease / 中国实用护理杂志

Objective To investigate the relationship between sleep beliefs and attitudes and sleep quality in patients with stable coronary heart disease. Methods Totally 390 patients with stable coronary heart disease were investigated by the Pittsburgh Sleep Quality Index (PSQI), Dysfunctional Beliefs and Attitudes about Sleep Scale-16(DBAS-16) and a general information questionnaire which was designed by researchers. Results 48.2% of patients with stable coronary heart disease reported poor sleep quality at home, and the total score of PSQI was (7.99 ± 4.061), the total score of DBAS was (47.44 ± 9.77). Univariate anova showed that age (F=14.567, P＜0.01), gender (H=16.657, P＜0.01), marital status (F=4.706, P=0.031), job status (F=15.738, P＜0.01) and the number of comorbid diseases (F=4.674, P=0.003),are factors affecting sleep quality. Pearson correlation analysis showed that there were significantly negative correlation between PSQI and DBAS (r=-0.260, P＜0.01). Multiple Linear Regression showed that sleep beliefs and attitudes, gender, age, the number of comorbid diseases were influencing factors of sleep quality (R2=0.247, F=17.907, P＜0.01). Conclusions The sleep quality at home in patients with stable coronary heart disease was closely related to sleep beliefs and attitudes. Nurses should pay more attention to the sleep quality of patients, correct their incorrect sleep cognition timely, and improve their sleep quality.

Research advances in the treatment of nonalcoholic fatty liver disease / 临床肝胆病杂志

Nonalcoholic fatty liver disease (NAFLD) has become the leading cause of abnormal liver enzymes in healthy individuals in China. Due to the complex pathogenesis of NAFLD, no consensus has been reached on the treatment of this disease. However, in recent years, more and more studies have put forward more pathogeneses and corresponding treatment recommendations. This article introduces and summarizes the research advances in the treatment of NAFLD, including lifestyle intervention, drug treatment, eradication of Helicobacter pylori, surgical treatment, regulation of intestinal microecology, and gene therapy.

Advances in Treatment of Intestinal Flora Imbalance in Ulcerative Colitis / 胃肠病学

Ulcerative colitis (UC) is a chronic,non-specific inflammatory disease mainly involving the colorectum.The main clinical manifestations are mucopurulent bloody stool,abdominal pain,diarrhea.The etiology of UC is not yet clear,and its treatment has become the focus of clinical work.Recent studies have confirmed that there is a certain correlation between the pathogenesis of UC and intestinal flora imbalance.Large number of clinical studies have been performed on the efficacy of probiotics and fecal microbiota transplantation (FMT) for UC.This paper reviewed the research status of treatment of intestinal flora imbalance in UC.

Survey of economic burden of hepatitis B-related diseases in 12 areas in China / 中华流行病学杂志

Objective Less surveys on the economic burden of hepatitis B (HB)-related diseases have been conducted in China,so the socioeconomic harm caused by the diseases is not clear and the key parameters for economic evaluation of hepatitis B prevention and treatment are lacking.This study aimed to analyze the direct,indirect and intangible expenditures of hospitalized patients with HB-related diseases during hospitalization and during a year in different areas of China.Methods The hospitals for infectious diseases and the large general hospitals in 12 areas in China were selected in the study.All the inpatients with HB-related diseases were surveyed by cluster sampling of consecutive cases.The direct expenditure included direct medical cost and direct non-medical cost.The indirect expenditure,including work loss of patients and caregivers,were calculated by using human capital method for urban and rural populations in 12 areas.The intangible expenditure were reflected by willing to pay and stochastic tournament.The influencing factors of direct and indirect costs were identified by stepwise linear multi-variation regression analysis.Results A total of 27 hospitals in 12 areas were included in the survey.A total of 4 718 cases were surveyed,the overall response rate was 77.7％.The average hospital stay was 29.2 days (27-34) and the hospitalization expenditure was averagely 16 832.80 yuan (RMB) per case,in which the highest proportion (61.2％)was medicine fees [10 365.10 yuan (RMB)].The average direct expenditure and indirect expenditure were consistent with the severity of illness,which were 18 336.10 yuan (RMB) and 4 759.60 yuan (RMB) respectively,with the ratio of 3.85:1.The direct medical expenditure [17 434.70 yuan (RMB)] were substantially higher than the direct non-medical expenditure [901.40 yuan (RMB)].It was found that the hospitalization expenses was highest in direct medical expenditure and the transportation expenses was highest in direct non-medical expenditures.Among the average indirect expenditure,the loss of income for the patients [3 832.50 yuan (RMB)] was higher than that for the caregivers [927.20 yuan (RMB)],The total direct and indirect expenditure was highest for liver transplantation,followed by severe hepatitis,hepatocellular carcinoma and decompensated cirrhosis,acute hepatitis B,compensated cirrhosis and chronic hepatitis B.The influencing factors for both direct and indirect expenditure were high hospital level,severity of hepatitis B,living in urban area,antiviral therapy,long hospitalization and monthly income of family.For average 3.74 outpatient visits and 1.51 hospitalization,the average annual direct,indirect and intangible expenditure for HB-related diseases were 30 135.30,6 253.80 and 44 729.90 yuan (RMB) [totally 81 119.00 yuan (RMB)],accounting for 37.3％,7.7％ and 55.0％,respectively.Of the annual direct medical expenditure [28 402.80 yuan (RMB)],which were much higher than non-medical expenditure [1 732.50 yuan (RMB)],hospitalization expenditure [26 074.20 yuan (RMB)] was higher than outpatient visit expenditure [4 061.10 yuan (RMB)].The annual indirect expenditures for outpatient visit and hospitalization were 763.60 and 5 490.10 yuan (RMB),respectively.Of the annual intangible expenditure,the highest was that for/primary hepatocellular carcinoma,followed by cirrhosis,chronic hepatitis B,severe hepatitis B,liver transplantation and acute hepatitis B.Conclusions A heavy economic burden has been caused by HB-related diseases in China,and patients are more likely to rely on medical service rather than non-medical service.It is necessary to take effective treatment measures to prevent the adverse outcome of HB related diseases and achieve significant economic benefits.The influence of HB related diseases on mental health of the people can be reflected by an economics term,intangible expenditure.

Survey of economic burden of hepatitis B-related diseases in 12 areas in China / 中华流行病学杂志

Objective Less surveys on the economic burden of hepatitis B (HB)-related diseases have been conducted in China,so the socioeconomic harm caused by the diseases is not clear and the key parameters for economic evaluation of hepatitis B prevention and treatment are lacking.This study aimed to analyze the direct,indirect and intangible expenditures of hospitalized patients with HB-related diseases during hospitalization and during a year in different areas of China.Methods The hospitals for infectious diseases and the large general hospitals in 12 areas in China were selected in the study.All the inpatients with HB-related diseases were surveyed by cluster sampling of consecutive cases.The direct expenditure included direct medical cost and direct non-medical cost.The indirect expenditure,including work loss of patients and caregivers,were calculated by using human capital method for urban and rural populations in 12 areas.The intangible expenditure were reflected by willing to pay and stochastic tournament.The influencing factors of direct and indirect costs were identified by stepwise linear multi-variation regression analysis.Results A total of 27 hospitals in 12 areas were included in the survey.A total of 4 718 cases were surveyed,the overall response rate was 77.7％.The average hospital stay was 29.2 days (27-34) and the hospitalization expenditure was averagely 16 832.80 yuan (RMB) per case,in which the highest proportion (61.2％)was medicine fees [10 365.10 yuan (RMB)].The average direct expenditure and indirect expenditure were consistent with the severity of illness,which were 18 336.10 yuan (RMB) and 4 759.60 yuan (RMB) respectively,with the ratio of 3.85:1.The direct medical expenditure [17 434.70 yuan (RMB)] were substantially higher than the direct non-medical expenditure [901.40 yuan (RMB)].It was found that the hospitalization expenses was highest in direct medical expenditure and the transportation expenses was highest in direct non-medical expenditures.Among the average indirect expenditure,the loss of income for the patients [3 832.50 yuan (RMB)] was higher than that for the caregivers [927.20 yuan (RMB)],The total direct and indirect expenditure was highest for liver transplantation,followed by severe hepatitis,hepatocellular carcinoma and decompensated cirrhosis,acute hepatitis B,compensated cirrhosis and chronic hepatitis B.The influencing factors for both direct and indirect expenditure were high hospital level,severity of hepatitis B,living in urban area,antiviral therapy,long hospitalization and monthly income of family.For average 3.74 outpatient visits and 1.51 hospitalization,the average annual direct,indirect and intangible expenditure for HB-related diseases were 30 135.30,6 253.80 and 44 729.90 yuan (RMB) [totally 81 119.00 yuan (RMB)],accounting for 37.3％,7.7％ and 55.0％,respectively.Of the annual direct medical expenditure [28 402.80 yuan (RMB)],which were much higher than non-medical expenditure [1 732.50 yuan (RMB)],hospitalization expenditure [26 074.20 yuan (RMB)] was higher than outpatient visit expenditure [4 061.10 yuan (RMB)].The annual indirect expenditures for outpatient visit and hospitalization were 763.60 and 5 490.10 yuan (RMB),respectively.Of the annual intangible expenditure,the highest was that for/primary hepatocellular carcinoma,followed by cirrhosis,chronic hepatitis B,severe hepatitis B,liver transplantation and acute hepatitis B.Conclusions A heavy economic burden has been caused by HB-related diseases in China,and patients are more likely to rely on medical service rather than non-medical service.It is necessary to take effective treatment measures to prevent the adverse outcome of HB related diseases and achieve significant economic benefits.The influence of HB related diseases on mental health of the people can be reflected by an economics term,intangible expenditure.

Barium-based Colonic Transit Detection and Individualized Treatment for Chronic Constipation / 胃肠病学

Background:Chronic constipation is a major cause of impaired quality of life in modern society. Reasonable and effective management of chronic constipation could be achieved based on the principle of evidence-based medicine and the modern concept of constipation,and this is a challenge facing the clinicians. Aims:To investigate the role of barium-based colonic transit detection in diagnosis and treatment of chronic constipation. Methods:Fifty patients with chronic constipation from Apr. 2013 to Oct. 2014 at the First Hospital of Shanxi Medical University were recruited and randomly allocated into two groups,control group and individualized treatment group. Patients in individualized treatment group received 20 barium markers orally and abdominal plain radiography was performed 48 and 72 hours later,respectively for calculating the colonic transit index. According to the type of colonic transition and the characteristics of colonic motility estimated by colonic transit index and clinical manifestations,an individualized therapeutic regimen was formulated and the efficacy was evaluated. Patients in control group were treated empirically according to the clinical manifestations. Results:Mosapride and lactulose or polyethylene glycol were administered orally in control group;when abdominal pain or abdominal distension was predominant,pinaverium bromide or trimebutine was used respectively instead of mosapride. Barium-based colonic transit detection revealed that 9 patients in individualized treatment group were slow transit constipation,6 were outlet obstructive constipation and 8 were the mixed type. After 2 weeks of empirical or individualized treatment,the defecation rates of the two groups were 24. 0%(6 / 25)and 52. 2%(12 / 23)within 24 hours and 64. 0%(16 / 25)and 87. 0%(20 / 23)within 48 hours,respectively(P all < 0. 05). Conclusions:Barium-based colonic transit detection is a simple,economical and practical modality for guiding the individualized treatment in patients with chronic constipation.

Effect of salvianolate injection on serum levels of pregnancy associated plasma protein A , brain natriuretic peptide and high sensitively C-reactive protein in elderly patients with acute myocardial infarction / 中华老年医学杂志

Objective To observe the effects of salvianolate injection on blood levels of high sensitively C-reactive protein (hs-CRP),pregnancy associated plasma protein-A (PAPP-A) and brain natriuretic peptide (BNP) in elderly patients with acute myocardial infarction.Methods The elderly patients with AMI (AMI group,n=160) and healthy controls (control group.n=30) were enrolled in this study and their blood concentrations of PAPP A,hs CRP and BNP were detected before and two weeks after treatment.The elderly patients in AMI group were randomized into conventional treatment group (n =80) and salvianolate group (n =80).Results The levels of PAPP-A,hs-CRP and BNP were significantly higher in AMI patients [(12.88±2.56) mg/L,(20.13 ±5.35) mU/L,(412.0±69.5) ng/L,respectively] than in healthy subjects[(1.20±0.88) mg/L,(1.90±0.46) mU/L,(89.0±5.6) ng/L,respectively] (t=24.670,3.780,11.939,respectively,P ＜0.01).But,before treatment there were no significant differences in the levels of PAPP-A,hs-CRP and BNP between the AMI group and control group (t=0.864,0.712,0.985,all P＞0.05).After two weeks of treatment,as compared with control group,AMI group showed that the serum concentrations of PAPP-A,hs-CRP and BNP were decreased significantly (P＜0.05).The levels of PAPP A,hs-CRP and BNP were (3.83±1.20) mg/L,(1.33±0.38) mU/L,(105.0±31.2) ng/L in salvianolate group and (5.71± 1.93) mg/L,(1.81±0.72) mU/L,(150.0±36.7) ng/L in conventional treatment group respectively,and the decrements in levels of PAPP-A,hs CRP and BNP were greater in the former than in the latter(t=7.399,5.273,8.356,respectively,all P＜0.05).Conclusions The dynamic serum concentrations of PAPP-A,hs CRP and BNP can be used as clinical indexes for the prognosis of acute myocardial infarction.Salvianolate injection can significantly decrease the serum levels of PAPP A,hs CRP and BNP.The salvianolate injection may have anti inflammatory effect and improve cardiac function in elderly patients with acute myocardial infarction,but the mechanism is still to be further discussed.

Expressions and relativity research of Cyclic B1 and Cyclic D1 in severe reflux esophagus, Barrett's esophagus and esophageal adenocarcinoma / 肿瘤研究与临床

Objective To explore the role of Cyclin B1 and Cyclin D1 on Barrett esophagus,Barrett's esophagus mixed with atypical dysplasia and esophageal adenocarcinoma.Methods Cyclin B1 and Cyclin D1 were examined with immunohistochemistry.76 esophageal tissues of patients werB collected,including severe reflux esophagitis(RE,25 cases),Barrett esophagus(BE,35 cases),Barrett esophagus mixed with atypical dysplasia(DY,8 cases),esophageal carcinoma(CA,8 cases).Ten cases with normal esophageal mucosa were examined as the control. Results Cyclin B1 and Cyclin D1 were high expression in the specimens of the BE,DY and CA groups and very low expression in the control and RE group.Statistieal difference Was showed(P<0.01).Expression of Cyclin D1 was increasing gradually from the tissues of intestinal metaplasia,atypical dysplasia to adenocarcinoma(50.04 vs 67.94 vs 74.31).There Was significant difference among these three groups(P<0.01).Conclusion Cyclin B1 and Cyclin D1 as markers of tumour development could evaluate the risk from Barrett esophagus to adenocarcinoma.Perhaps it is the earlv event in the development of esophageal carcinoma.

13C-methacetin breath test as a quantitative liver function assessment in non-alcoholic fatty liver / 中国医师杂志

Objective To investigate and assess 13C-methacetin breath test as a method to quantitatively evaluate the liver function of non-alcoholic fatty liver. Methods Twenty-four male Wistar rats were randomly divided into the following 2 groups, control group given a standard chow and model group given high-fat diet (88 % standard chow + 10% lard +2% cholesterol). At the 8th week and 12th week, the isotope-selective nondispersive infrared spectrometer (NDIRS) was applied to 13C-methacetin breath test. Peak value of breathing (DOB), the duration to peak (T), and cumulative expiration within 60 min (CUM60min) were determined. Then, the two groups of rats were executed respectively. The level of endotoxin in the portal vein and abdominal aorta was detected respectively, and the specific tissue of liver was fixed in 10% buffered formalin, processed, and embedded in paraffin for hematoxylin eosin (H&E). Results Compared with the control group, DOB increased in the model group at the 12th week but not at the 8th week. The trend was corresponded to the degree of path-ological lesion in the liver of rats. At the 8th week and 12th week, endotoxin levels in the portal vein of model group were significantly in-creased compared with control group. The breath test results were positively related to emlotoxin levels in the portal vein. Conclusion 13C-methacetin breath test may be a non-invasive method to evaluate the pathological changes of non-alcoholic fatty liver.

The study on the apoptosis of introduction p53 gene alone and incombination with TNF-? into Hep3B / 肿瘤研究与临床

Objective To investigate the suppression effects of tumor suppressor gene p53 alone and apoptosis incombination with TNF-? in a hepatocellular carcinoma cell line Hep3B. Methods Hep3B cells were transfected with a wild-type p53 cDNA(wt-p53)and plain vector(pNeo) respectively. Then the cell were cultured for 12 hours, one of the transfected p53 groups was added TNF-?(20 ?g/ml). The expression of p53 was detected by immunological fluorescence assay. Determination of apoptosis was used by DNA fragmentation, TUNEL assay. Results Both a small dose TNF-? and wt-p53 can induce apoptosis more efficiently comparing with non-transfected cultures(P

Study on liver fibrosis of chronic hepatitis B with ultrasound tissue characterization / 中华超声影像学杂志

Objective To study a kind of quantitative diagnosis method with ultrasound tissue characterization on liver fibrosis of the patients with chronic hepatitis B.Methods Measuring the gray scales of the two dimensional ultrasonography of the patients and compare the gray scales with the fibrosis degrees on histopathology of the patients and finding the differences among every two groups.Results The differences of the gray scales compared with the fibrosis degrees on histopathology were meaningful on statistics( F = 34.3, P