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Objective To investigate the comparation of sodium valproate concentration in peripheral blood monitoring by fluorescence polarization immunoassay method(FPIA)and high performance liquid chromatography method(HPLC)in epilepsy children.Methods 87 cases of epilepsy children received Sodium valproate treatment in our hospital from February 2014 to June 2016 were selected,fasting venous blood of elbow vein were collected the next morning after last medication, blood concentrations of Sodium valproate in serum samples were detected by FPIA method and HPLC method respectively,the correlation and consistency of results of the two methods were observed and compared.Results The intra day and inter day RSD of Sodium valproate concentration in peripheral blood in epilepsy children detected by FPIA were <5%,the recovery rate was 90%-110%,the precision and accuracy were high;The intra day and inter day RSD of Sodium valproate concentration in peripheral blood in epilepsy children detected by HPLC were <5%,the recovery rate was 90%-110%,the precision and accuracy were high; the linear regression equation between determination value of HPLC method (X) and determination value of FPIA method (Y) was:Y=0.8355X+1.8231,correlation coefficient r=0.914,the detection results were positively related;the Sodium valproate blood concentration detected by FPIA was significantly lower than that detected by HPLC method, the difference was statistically significant (P<0.05);Bland-Altman deviation chart results show that determination of blood drug concentration by HPLC method was higher than that of FPIA method by 7.2μg/mL.Conclusion The precision and accuracy of sodium valproate concentration in peripheral blood monitoring by FPIA method and HPLC method were all high, and the correlation was good, but the detection results of the two methods were significantly different,the detection result of HPLC method was higher than that of FPIA method,need to choose and judge according to the clinical situation.
RESUMO
@#ObjectiveTo study the relationship between type of vertebral fracture and degree of spinal cord injury.MethodsMRI signs of 200 cases of thoracolumbar SCI were analyzed retrospectively.ResultsAmong all cases, 49 cases were with compressed fracture, 60 cases with burst fracture, 79 cases with fracture and dislocation, 158 cases with angle formed posteriorly. The number of cases with complete SCI were respectively 14 cases, 39 cases, 54 cases and 94 cases.Conclusions Common sites of thoracolumbar SCI. were at T12 and L1 level (123 cases, 74%). The degree of SCI was related to the type of vertebral fracture. The complete SCI occurred most frequently in patients with burst fracture and patients with fracture and dislocation, the incidence of the latter was higher.