The role and molecular mechanism of miR-494 in disease development and progression / 生理学报

MicroRNA-494 (miR-494) is a small non-coding RNA located in chromosome 14q32.31 and regulates post-transcriptional gene expression by promoting the degradation of its target mRNAs via binding to the 3' untranslated regions (3'UTR). It has been reported that miR-494 plays an important role in the occurrence, development and prognosis of various diseases. Several signaling pathways modulated by miR-494 including the PTEN/PI3K/AKT, nuclear factor κ-B (NF-κB), mitogen-activated protein kinase (MAPK), transforming growth factor-β (TGF-β)/SMAD, and Wnt/β-catenin are associated with physiological regulation and pathological process in many diseases. The stably expression of miR-494 in the blood stream suggests its potential as a biological marker for disease diagnosis, treatment, and prognosis. Based on recent research, we summarize the role and molecular mechanism of miR-494 in disease development and progression. We also discuss its potential as a marker for clinical diagnosis and prognosis of various diseases.

Ocular surface changes after combined surgery of glaucoma and cataract / 国际眼科杂志(Guoji Yanke Zazhi)

·AIM: To investigate the effect of phacoemulsification intraocular lens implantation combined with trabeculectomy on ocular surface in the treatment of glaucoma and cataract. ·METHODS: Totally 45 cases of patients with glaucoma and cataract in our hospital from February 2016 to February 2017 were enrolled. All the patients had unilateral disease,and the medical records of the patients were analyzed retrospectively. The sicked eyes were set as the observation group and contralateral eyes were set as the control group. The dry eye score, break-up time (BUT), corneal fluorescein staining score (FL), tear secretion test (S I t) and conjunctival epithelium goblet cell density(CIC) was compared between two groups before operation and at 1wk,1 and 3mo after operation. · RESULTS: The preoperative dry eye score of the observation group had no significant difference with that of the control group (P > 0. 05). At 1wk, 1mo after operation,data of the observation group was significantly higher than that of the control group and before operation, with statistical significance (P < 0. 05), no significant difference was shown at postoperative 3mo (P>0.05). Preoperative BUT of the observation group had no significant difference with that of the control group(P>0.05); at 1 wk,1 and 3mo after operation, data of the observation group was significantly lower than that of the control group and before operation, with statistical significance (P<0.05). The preoperative FL score of the observation group was significantly higher than that of the control group, while the postoperative FL score of the observation group at 1wk,1 and 3mo had sharp difference with that before operation(P<0.05),all were significantly higher than that of the control group,there was statistical significance(P<0.05). There was no significant difference in S I t score between the observation group and the control group preoperatively ( P > 0. 05), and the postoperative S I t scores of the observation group at 1wk,1 and 3mo postoperatively was evidently lower than that of the control group and before operation (P<0.05). There was no significant difference in CIC density between the two groups preoperatively. While the postoperative data of the observation group at 1wk, 1 and 3mo postoperatively was significantly lower than that of the control group and before operation,with statistical points (P<0.05). · CONCLUSION: Phacoemulsification intraocular lens implantation combined with trabeculectomy for treatment of glaucoma and cataract can aggravate dry eye symptoms, as well as reduce tear film stability and tear secretion,impair conjunctival epithelium goblet cell.

Clinical observation of Gemcitabine combined with Tiggine chemotherapy and psychological intervention after radical operation of pancreatic cancer / 中国生化药物杂志

Objective To discuss the clinical effect of Gemcitabine combined with Tiggine chemotherapy and psychological intervention after radical resection of pancreatic cancer. Methods 84 patients for radical resection of pancreatic cancer in Taizhou Enze hospital from March 2015 to October 2016 were randomly divided into the experimental group (42 cases) and the control group (42 cases), the control group after treated was given gemcitabine plus paclitaxel chemotherapy, the experimental group was received Gemcitabine combined with Tiggine chemotherapy plus psychological intervention, the effect and quality of life between two groups of patients were compared and analysed. Results The quality of life of the patients for 2 weeks, 4 weeks, 8 weeks and 12 weeks were significantly better than the control group with statistical significance (P<0.05). sFlt-1 in the experimental group was higher than the control group, the VEGF level is lower than the control group, the differences between two groups were statistically significant (P<0.05). Conclusion Gemcitabine combined with Tiggine chemotherapy plus psychological intervention after radical resection of pancreatic cancer, could effectively improve the treatment efficiency and the quality of life, and be worthy of promotion and application.

Clinical observation of Gemcitabine combined with Tiggine chemotherapy and psychological intervention after radical operation of pancreatic cancer / 中国生化药物杂志

Objective To discuss the clinical effect of Gemcitabine combined with Tiggine chemotherapy and psychological intervention after radical resection of pancreatic cancer. Methods 84 patients for radical resection of pancreatic cancer in Taizhou Enze hospital from March 2015 to October 2016 were randomly divided into the experimental group (42 cases) and the control group (42 cases), the control group after treated was given gemcitabine plus paclitaxel chemotherapy, the experimental group was received Gemcitabine combined with Tiggine chemotherapy plus psychological intervention, the effect and quality of life between two groups of patients were compared and analysed. Results The quality of life of the patients for 2 weeks, 4 weeks, 8 weeks and 12 weeks were significantly better than the control group with statistical significance (P<0.05). sFlt-1 in the experimental group was higher than the control group, the VEGF level is lower than the control group, the differences between two groups were statistically significant (P<0.05). Conclusion Gemcitabine combined with Tiggine chemotherapy plus psychological intervention after radical resection of pancreatic cancer, could effectively improve the treatment efficiency and the quality of life, and be worthy of promotion and application.

Quality of life of inpatients with coal workers' pneumoconiosis and its influential factors / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To investigate the quality of life ( QOL) of inpatients with coal workers' pneumoconiosis( CWP) and analyse its influential factors, and to provide a theoretical basis for effective control measures.</p><p><b>METHODS</b>Eighty-eight CWP patients in a hospital were included in the study. A questionnaire survey was conducted in them using a self-designed QOL scale. A database was established by software EpiData3.1, and the obtained data were statistically analyzed by software SPSS 16.0.</p><p><b>RESULTS</b>Of the 88 patients, 73( 82.9%) had middle-level QOL, with a mean QOL loss rate of 36.2%; the loss rates of physical function and somatic sensation were the highest ( 44.2% and 41.5%). The patients with stage II CWP had significantly lower physical function than those with stage I and III CWP; the physical function and social function of patients significantly decreased with age; the personal income, household income, and housing condition of the patients had a marked impact on their physical and psychological functions, and the housing condition and education level had a marked impact on their social function. The multivariate analysis showed that old age, low income,and poor housing condition were the main adverse factors for the QOL of inpatients with CWP.</p><p><b>CONCLUSION</b>The QOL of inpatients with CWP declines significantly, and their QOL is related to the age, income, and satisfaction with housing condition.</p>

Silica induced α-SMA expression in HBE cell line by targeting the PI3K/Akt pathway / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To explore the role of the phosphatidylinositol 3 kinase/protein kinase B (PI3K/Akt) pathway in silica-induced α-SMA (α smooth muscle actin) expression in HEB (human bronchial epithelial) cell.</p><p><b>METHODS</b>The cultured HBE cells were divided into 5 groups: control, silica, PI3K inhibitor (Ly294002), both PI3K inhibitor (Ly294002) and silica at the same time and the inhibitor 24 h ahead of silica. The final concentrations of PI3K inhibitor and silica were 10 µmol/L and 100 µg/ml, respectively. Western blots were used to detect protein expressions of Akt, p-Akt, TGF-β and α-SMA. The location and expression of α-SMA were measured by immunofluorescence assay.</p><p><b>RESULTS</b>HBE cell line exposed to silica can induce Akt phosphorylation, in which expressions of p-Akt were up regulated 1 times at 48 and the highest at 72 h. The expressions of TGFβ increased remarkably at 12 h and the peak at 48 h after silica exposure, while the expressions of α-SMA increased at 24 h and the highest at 72 h. However, the PI3K inhibitor (Ly294002) significantly down regulated α-SMA expression. When the cell line exposed to the PI3K inhibitor ahead of silica 24 h, the expressions of p-Akt and α-SMA were more remarkably down regulated which were decreased 1.5 times and 7.6 times respectively compare to silica exposure group. But no significant changes were found for TGFβ expressions. The immunofluorescence assay showed that silica can induce α-SMA expression, which located in cytoplasma, and PI3K inhibitor can decrease the expression.</p><p><b>CONCLUSIONS</b>Silica induced α-SMA expression in HBE cell line is by targeting the PI3K/Akt pathway and PI3K inhibitor can repress α-SMA expression.</p>