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Objective To investigate the general situations of gravida,pregnancy complications,childbirth and neonatal outcomes of twin pregnancies conceived by assisted reproductive techniques (ART) and those conceived spontaneously.Methods A retrospective analysis was carried out on the basic information,perinatal complications,delivery information and neonatal outcomes of twin pregnancies received by ART (ART group,n=518) and those conceived spontaneously (SC group,n=293).Results Gravida age was older in ART group than in SC group (P＜0.05),while the gravidity and parity in ART group was more than that in SC group (P＜0.05).There were more embryos and high proportion of diamniotic cyst in ART group than in SC group (P＜0.05).The gestational incidence of diabetes,abnormal placenta (placental adherence,placenta accrete,placenta previa) and postpartum hemorrhage were higher in ART group than in SC group (P＜0.05),while no statistically significant differences existed between the two groups in the incidence of gestational complications (such as gestational hypertension disease,cholestasis and amniotic fluid volume abnormality) and in neonatal outcomes including neonatal weight,incidence of asphyxia and congenital malformation and neonatal death (P＞0.05).Conclusion Twin pregnancy conceived by ART may lead to higher incidences of gestational diabetes mellitus and abnormal placenta and more postpartum hemorrhage,but no significant difference existed in the neonatal outcomes between twin pregnancies conceived by ART and those conceived spontaneously.
RESUMO
Objective To study the influence of HBV-DNA with different load levels of HBsAg-positive among fathers on the rate of neonatal cord blood HBV-DNA.Methods Using HBsAg and HBV-DNA as screening indicators for pregnant women and their husbands from an obstetric clinic.161 pregnant women whose HBsAg and HBV-DNA were negative,but HBsAg was positive among their husbands and their newborns,were selected.Blood samples from those pregnant women,their husbands and their newborns were collected to detect the related indicators.Using ELISA to detect hepatitis B virus markers(HBVM),and FQ-PCR to detect the levels of HBV-DNA load.According to neonatal cord blood HBV-DNA detection guideline,newborns with cord blood HBV-DNA positive were selected as cases,others as controls.Results(1)Result of the study showed that there was a dose-response relationship between paternal serum HBV-DNA load levels and neonatal cord blood HBV-DNA positive rates in newborns(trend χ~2=64.117,P=0.000).The rate of vertical transmission of HBV from HBsAg-positive father to infant in the paternal serum HBV-DNA＞1.0×107 copies/ml group was significantly higher than HBV-DNA＜1.0×107 copies/ml group(χ~2=71.539,P=0.000).(2)There was a positive rank correlation between semen positive HBeAg and vertical transmission of HBV from HBsAg-positive father to infant(χ~2=6.892,P=0.009).Conclusion There was a dose-response relationship between paternal serum HBV-DNA load levels and neonatal cord blood HBV-DNA positive in newborns.Paternal serum HBV-DNA≥1.0×107 copies/ml and with HBeAg positive status were risk factors of vertical transmission of HBV from HBsAg-positive father to infant.