Two new labdane diterpenoids from the leaves of Callicarpa formosana Rolfe / 药学学报

Two new labdane diterpenoids were isolated from 95% ethanol extract of the leaves of Callicarpa formosana Rolfe by using silica gel column, MCI column, ODS column and HPLC. Their structures were elucidated by HR-ESI-MS, NMR and ECD spectral data. All of them are new compounds, named 13E-6β-hydroxylabda-8(17),13-dien-15-oic acid (1) and 13E-7α-hydroxylabda-8(17),13-dien-15-oic acid (2). Compounds 1 and 2 were tested for antioxidant activity, and none of them had obvious activity.

A new carbazole alkaloid from the stems of Clausena lansium / 药学学报

A new carbazole alkaloid was isolated from the aqueous extract of the stems of Clausena lansium (Lour.) Skeels by various chromatographic methods, including HPD-100, PRP-512A, silica gel, and reverse phase C18. Its structure was determined by spectroscopic and chemical methods, including UV, IR, HR-ESI-MS, 1D/2DNMR and ECD. Compound 1, named as Claulamine F, showed no antimicrobial activity on Staphylococcus aureus, Escherichia coli or Pseudomonas aeruginosa. In addition, compound 1 exhibited no cytotoxicity on five kinds of cancer cells through MTT methods.

Flavonoids from the aerial parts of Lespedeza cuneata / 药学学报

The chemical constituents of the aerial parts of Lespedeza cuneata (Dum. Cour.) G. Don were investigated using chromatographic techniques, such as silica gel, reversed phase MPLC and preparative HPLC. Five compounds were isolated and their structures were elucidated by spectroscopic data and physicochemical properties, which were identified as 7-O-glucosyllaburnetin (1), kaempferol-3-O-β-D-galactopyranoside (2), kaempferol-3-O-α-L-rhamnoside (3), vitexin (4), and isovitexin (5). Among those, compound 1 is a new compound, compounds 2-3 were isolated from this plant for the first time. Compounds 1-5 were tested for their anti-ulcerative colitis activity by dual luciferase report gene assay targeting xbp1. Compared with control group, compound 1 showed a certain activity on activating the transcription of xbp1, with its relative activating ratio being 1.80 times.

Triterpenes from fruits of Paliurus ramosissimus / 药学学报

To study the chemical constituents of the fruits of Paliurus ramosissimus, various chromatographic techniques were used to separate and purify the chemical constituents. Three triterpenes have been isolated and purified by using various column chromatography. Their structures were elucidated by their physico-chemical properties and spectroscopic data. These compounds were determined as: 22S, 23R-epoxy-tirucalla-7-ene-3alpha,24, 25-triol (1), 21S, 23R-epoxy-21, 24S, 25-trihydroxy-apotirucalla-7-ene-3-one (2), 21R, 23R-epoxy-21-ethoxy-24S, 25-dihydroxy-apotirucalla-7-ene-3-one (3), separately. Compound 1 is a new compound, and the others were obtained from this genus for the first time.

Studies on chemical constituents in root of Isatis indigotica / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents in the root of Isatis indigotica.</p><p><b>METHOD</b>The constituents root were separated through various chromatographic techniques and their structures were elucidated by means of physicochemical properties and the analysis of their spectral data.</p><p><b>RESULT</b>Eleven compounds were isolated and identified as (+) -isolariciresinol (1), lariciresinol (2), lariciresinol-9-O-beta-D-glucopyranoside (3), lariciresinol-4'-O-beta-D-glucopyranoside (4), lariciresinol-4,4'-bis-O-beta-D-glucopyranoside (5), 3-formylindole (6), 1-methoxy-3-indolecarbaldehyde (7), 1-methoxy-3-indoleacetonitrile (8), deoxyvasicinone (9), epigoitrin (10), adenosine (11).</p><p><b>CONCLUSION</b>Compounds 4-8 were isolated from I. indigotica for the first time.</p>

Studies on chemical constituent of Solanum lyratum / 中国中药杂志

<p><b>OBJECTIVE</b>To study chemical constituents of Solanum lyratum.</p><p><b>METHOD</b>The constituens were isolated by polystyrene resin RA, sephadex LH-20 and silica gel column chormatography, their structures were identified by spectroscopic analysis.</p><p><b>RESULT</b>Four compounds were identified as The constituens were caffeic acid, vanillic acid, rutin and N(p-hydropheneethyl) p-coumaramide.</p><p><b>CONCLUSION</b>These compounds were isolated from this plant for the first time.</p>