Integrin 3 mRNA changes after orthodontic teeth movement in periodontitis rats / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To study the integrin beta3 mRNA changes after orthodontic treatment on normal teeth and periodontitis teeth in rats.</p><p><b>METHODS</b>96 adult SD rats of 10 weeks old were randomly divided into normal tooth move-ment group and periodontitis tooth movement group. The rats in the two groups were sacrificed after 0 d, 12 h, 1 d, 3 d, 5 d and 7 d of tooth movement. The alveolar specimens were prepared. The integrin beta3 mRNA were detected using in situ hybridization in the specimens. The OD index of positively stained osteoclasts for integrin beta3 mRNA after orthodontic tooth movement in the two groups were measured and compared.</p><p><b>RESULTS</b>There were weak positive signals on the cytoplasm of osteoclasts in periodontum in both groups after 12 hours and 3 days force activation. No positive signals were detected in the rest samples. There was no difference in the OD of positive stained osteoclasts between normal and periodontitis groups. Strong expressions were present on cells with one or two nuclei in the alveolar marrow.</p><p><b>CONCLUSION</b>It is suggested that integrin beta3 mRNA is related with osteoclasts maturation and migration in orthodontic tooth movement.</p>

Association of estrogen receptor gene polymorphisms and primary trigeminal neuralgia / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the association of estrogen receptor (ER) gene polymorphism and primary trigeminal neuralgia.</p><p><b>METHODS</b>By polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), ER gene polymorphism was analyzed in 20 trigeminal neuralgia (TR) patients and 20 control individuals, and the distribution of ER genotype was compared in TR group and control group.</p><p><b>RESULTS</b>There was no significant difference in frequencies of allele and genotype in XbaI or PvuII polymorphism or XbaI with PvuII polymorphisms together between TR group and control group (P > 0.05). The genotypic distribution of Xx or PpXx in TR group was higher than control group, and it was contary to xx, ppxx or Ppxx in TR group and control group.</p><p><b>CONCLUSION</b>XbaI or PvuII polymorphism may be related to TR. Women with PpXx genotype may be a dangerous factor to primary trigeminal neuralgia.</p>

The integrin beta1 mRNA changes after orthodontic movement of teeth in periodontitis rats / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To study the integrin beta1 mRNA changes after orthodontic tooth movement in normal teeth and periodontitis teeth of rats.</p><p><b>METHODS</b>The OD of positively stained osteoclasts for integrin beta1 mRNA using in situ hybridzation was detected after orthodontic tooth movement in normal teeth and periodontitis teeth groups.</p><p><b>RESULTS</b>Integrin beta1 mRNA expression were detected on all osteoclasts in tooth movement samples of normal and periodontitis teeth. There were stronger positive signals after given orthodontic force in both of the two groups. But no differences were found after 0.5, 1, 2, 3, 5, 7, 10 days since orthodontic tooth movement. The integrin beta1 mRNA signals in normal tooth movement group were not different from that in periodontitis group.</p><p><b>CONCLUSION</b>The integrin beta1 of osteoclasts may play a role in the stability and remodeling of periodontal ligament in orthodontic tooth movement. There were no difference in the OD of integrin beta1 mRNA staining in orthodontic tooth movement between normal teeth group and periodontitis teeth group.</p>

Effects of orthodontic force on tumor necrosis factor alpha protein expression in the inflammatory periodontal tissues / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To explore the combined effects of orthodontic force and inflammation on the remodelling of periodontal tissues.</p><p><b>METHODS</b>The upper first molars underwent mesial orthodontic force on 48 rats suffering experimental periodontitis and 48 rats injected lipopolysaccharide, respectively.</p><p><b>RESULTS</b>The TNF-alpha protein expression in the compressed periodontal tissues fluctuated during 0, 2, 12 hours and 2, 7, 14 days stages, the OD value got to the peak in the compressed periodontal tissues in 2 days.</p><p><b>CONCLUSION</b>The mechanical remodelling effects were hindered due to the circumstance of acute or chronic inflammation. The research suggests that the orthodontic treatment to adult patients with periodontal inflammation should be taken carefully.</p>