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Chinese Journal of Industrial Hygiene and Occupational Diseases ; (12): 589-592, 2013.
Artigo em Chinês | WPRIM | ID: wpr-275879

RESUMO

<p><b>OBJECTIVE</b>To investigate the expression of caspase-3, Bcl-2, and Bax in pentavalent vanadium-induced neuronal apoptosis and the neurotoxicity of pentavalent vanadium to in vitro cultured rat neurons.</p><p><b>METHODS</b>Neurons from rats were cultured in vitro and treated with different concentrations of V2O5. Neuronal apoptosis was evaluated by TdT-mediated dUTP-biotin nick end labeling (TUNEL). The expression of caspase-3, Bcl-2, and Bax in neurons was measured by Western blot. The images collected by gel imaging system and scanner were analyzed.</p><p><b>RESULTS</b>The TUNEL showed that compared with the control group, the middle- and high-dose exposure groups had significantly increased apoptosis index (AI) of neurons (P < 0.05 or P < 0.01). The Western blot showed that compared with the control group, the middle- and high-dose exposure groups had significantly increased expression of caspase-3 and Bax and significantly decreased expression of Bcl-2 (P <0.05 or P < 0.01). The AI of neurons was positively correlated with the expression of caspase-3 and Bax (r = 0.943, P < 0.01; r = 0.937, P < 0.01) and negatively correlated with the expression of Bcl-2 (r = -0.908, P < 0.01).</p><p><b>CONCLUSION</b>Pentavalent vanadium may induce neuronal apoptosis, and the expression of caspase-3, Bcl-2, and Bax, which regulate apoptosis, plays an important role in the neuronal apoptosis induced by pentavalent vanadium.</p>


Assuntos
Animais , Ratos , Apoptose , Caspase 3 , Metabolismo , Células Cultivadas , Neurônios , Metabolismo , Patologia , Proteínas Proto-Oncogênicas c-bcl-2 , Metabolismo , Ratos Sprague-Dawley , Vanádio , Toxicidade , Proteína X Associada a bcl-2 , Metabolismo
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