Effect of allogenic adipose-derived stem cell grafting on serum biochemical indicators and sporting ability of endurance trained rats / 南方医科大学学报

<p><b>OBJECTIVE</b>To observe the effect of transplantation of allogeneic adipose-derived stem cells (ADSCs) on serum biochemical indicators and sporting ability in highly endurance-trained rats from a fasciological perspective.</p><p><b>METHODS</b>The ADSCs were cultured in vitro. Forty male Wistar rats were randomized into 4 equal groups, namely the blank control group, overtraining (model) group, transplantation without training group and overtraining plus transplantation group. The rats in the two overtraining groups were subjected to exhaustive swimming for 1 week, and in the two transplantation groups, cultured allogeneic ADSCs (2×10(6)/ml) were injected via the tail vein. The exhaustion time in swimming and the serum levels of BUN, LDH, BLa, and Hb of the rats were recorded after the treatments.</p><p><b>RESULTS</b>The rats in the model group showed significantly increased serum BUN, LDH and BLa levels and decreased Hb level with a extended exhaustion time as compared with those in the blank control group (P<0.01). The BUN, LDH and BLa was significantly lower, Hb level higher and the exhaustion time significantly longer in the overtraining plus transplantation group than those in the model group (P<0.01).</p><p><b>CONCLUSIONS</b>ADSCs can effectively prolong the exhaustion time of rats during exhaustive swimming and enhance their sporting ability.</p>

Effect of allogenic adipose-derived stem cell transplantation on bone mass in rats with glucocorticoid-induced osteoporosis / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate the effects of systemic transplantation of allogenic adipose-derived stem cells (ADSCs), the main undifferentiated cells in the supporting-storing system based on the fasciology hypothesis, on the bone mineral density (BMD) and histomorphometry in rats with glucocorticoid-induced osteoporosis (GIOP) rats, and explore a new therapeutic approach for osteoporosis.</p><p><b>METHODS</b>Forty female adult Wistar rats were randomized equally into blank control group (A), model group (B), control treatment group (C) and treatment group (D). In groups B, C, and D, osteoporosis was induced by injection of prednisolone (8 mg/kg) via the tail vein 3 times a week for 12 consecutive weeks. After successful establishment of osteoporosis, allogenic ADSCs (3×10(6)/ml) were transplanted via the tail vein. The BMD at the L3-L5 levels and of the right femurs were detected, and the histomorphometry of the right tibias was analyzed in all the rats.</p><p><b>RESULTS</b>After prednisolone injection, the BMD of L3-L5 vertebrae and the right femurs, the percent trabecular area, trabecular thickness and trabecular number of the right tibias were all obviously lowered while the trabecular separation and osteoclast number increased in group B as compared to those in group A (P<0.05). Four weeks after ADSC transplantation, all these indices were significantly improved in group D, showing significant differences from those in group B (P<0.05) and also group C.</p><p><b>CONCLUSION</b>Transplantation of allogenic ADSCs can restore the BMD and bone histomorphometric properties of rats with GIOP, and may serve as a potential treatment for GIOP. These results also provide partial experimental evidence supporting the hypothesis of fasciaology.</p>

Three-dimensional design of surgery for mandibular retrusion / 南方医科大学学报

<p><b>OBJECTIVE</b>To explore the feasibility of surgical design for mandibular retrusion using three-dimensional software.</p><p><b>METHODS</b>Three-dimensional reconstruction was performed by Mimics software based on the preoperative CT data. The model of the maxillofacial region was imported into Rapidform software for measuring the associated parameters and Geomagic software for simulation of osteotomy. The reliability of the virtual operation was validated during the surgery.</p><p><b>RESULTS</b>The model of mandibular retrusion was reconstructed and successfully used to simulate the surgery. The simulation result was applied in subsequent actual surgery and good surgical outcomes were achieved.</p><p><b>CONCLUSION</b>The three-dimensional software can be used to simulate the surgery for mandibular retrusion and improve the predictability and accuracy of the surgery.</p>

Immunophenotypic comparison of adipose-derived mesenchymal stem cells from the greater omentums and subcutaneous adipose tissues of rats / 南方医科大学学报

<p><b>OBJECTIVE</b>To compare the adipose-derived mesenchymal stem cells (ADMSCs) isolated from the greater omentums and subcutaneous adipose tissues of rats for their characteristics in cell morphology, growth kinetics and immunophenotypes.</p><p><b>METHODS</b>ADMSCs were isolated from the greater omentums and inguinal fat pads of 6 SD rats and cultured in vitro. The morphologies of the ADMSCs were observed using phase-contrast microscopy, and their growth curves were generated and the doubling times determined. The phenotypic marker profiles including CD11b, CD29, CD45, CD49d, CD90 and CD106 of the ADMSCs in the fourth passage were determined using flow cytometry.</p><p><b>RESULTS</b>The ADMSCs harvested from the greater omentums and inguinal fat pads showed almost identical morphologies. The growth curves and the mean doubling time of the ADMSCs from the two different sources showed no obvious difference. With similar positivity rates for CD11b, CD29, CD106 and CD90, the two ADMSCs exhibited different expression rates of CD45 and CD49d.</p><p><b>CONCLUSIONS</b>The immunophenotypic characteristics of the ADMSCs from the greater omentums and subcutaneous adipose tissues are not totally identical.</p>

Expression of extracellular signal-regulated protein kinases in the subcutaneous fascia of rats and their changes after acupuncture / 南方医科大学学报

<p><b>OBJECTIVE</b>To observe the effect of acupuncture on the expression of extracellular signal-regulated protein kinases 1/2 (ERK1/2) in the subcutaneous fascia of SD rats.</p><p><b>METHODS</b>Eighteen SD rats were randomly divided into 6 groups (n=3) including 5 acupuncture groups and a control group. The rats in the 5 acupuncture groups received electro-acupuncture therapy in the regions of the inguinal groove, and at 0, 1, 6, 12, and 36 h after the last therapy, the superfacial fascia surrounding the acupuncture point (about 1.5 cm in diameter) were collected. The fascia tissues at the corresponding sites and at the acupoint Zusanli (ST36) were obtained from the control rats. The expression of ERK1/2 and phosphorylated ERK1/2 (p-ERK1/2) in the tissues were detected by Western blotting.</p><p><b>RESULTS</b>ERK1/2 and p-ERK1/2 expressions were detected in the tissues harvested from both the acupoint and the non-acupoint in the control rats with similar expression intensities. In the rats of each acupuncture group, ERK1/2 expression was significantly increased on the acupuncture side in comparison with the control side.</p><p><b>CONCLUSION</b>The normal loose connective tissue may participate in tissue proliferation and differentiation possibly via phosphorylation of ERK. Acupuncture can promote the signal transduction pathway of ERK, which can be a possible mechanism for the effect of acupuncture in modulating the physiopathological conditions.</p>

Biocompatibility between human adipose-derived mesenchymal stem cells and porcine bone scaffolds / 南方医科大学学报

<p><b>OBJECTIVE</b>To study the compatibility between human adipose-derived mesenchymal stem cells and porcine bone scaffolds.</p><p><b>METHODS</b>Porcine bone tissues were co-cultured with adipose-derived mesenchymal stem cells, and the complex was observed under scanning electron microscope. The viability and alkaline phosphatase (ALP) activity of the cells were examined with the cells co-cultured with human bone scaffold as the control.</p><p><b>RESULTS</b>At 4 and 10 days after the co-culture, the adipose-derived mesenchymal stem cells were observed to extend pseudopodia to adhere to the two scaffold materials. MTT assay showed that the cell proliferation on both of the materials increased with time, and the two cell complexes exhibited similar pattern of changes in ALP activity.</p><p><b>CONCLUSION</b>As the seed cells, human adipose-derived mesenchymal stem cells exhibit good comparability with porcine bone scaffold, suggesting their potential of constructing tissue-engineered bone graft.</p>

Variation of nestin expression in SD rat eyes in different stages of postnatal development / 南方医科大学学报

<p><b>OBJECTIVE</b>To observe the position and quantity of nestin expression in SD rat eyes in different stages of postnatal development.</p><p><b>METHODS</b>Immunocytochemical method was used to identify nestin expression in the eyes of SD rats of 1 to 30 days old.</p><p><b>RESULTS</b>Nestin expression was detected in the retina and extraocular muscles of SD rats. The expression varied with the time of postnatal development, distributing in the entire retina layers in earlier stages and confined in the nerve fiber layer in later stages. The quantities of nestin expression in the extraocular muscles decreased gradually with growth.</p><p><b>CONCLUSION</b>Nestin expression in the retinas and extraocular muscles of SD rats decreases during the postnatal development.</p>