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Sustained release and non-parental formulations of peptides and protein drugs are highly desirable because of enhanced therapeutic effects as well as improved patient compliance. This is especially true for small peptides such as thymopentin (TP5). To this end, implantable sandwich poly (hydroxybutyrate--hydroxyhexanoate) (PHBHHx) films were designed to prolong release time and to inhibit burst release phenomenon of TP5 by a simple volatilization method. release studies revealed that sandwich films had nearly no burst release. release time of sandwich films was prolonged to 42 days. Pharmacodynamic evaluation demonstrated that TP5 sandwich films significantly increased survival rates in a rat immunosuppressive model and normalized CD4/CD8 values. These results suggest that TP5 released from sandwich films can attenuate cyclophosphamide's immunosuppressive activity, and possibly achieve results comparable to daily TP5 injection therapy. Thus, sandwich PHBHHx films show excellent potential as a sustained, burst-free release system for small molecular weight, hydrophilic peptide drugs.
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Electrochemical immunosensors is widely used in clinical laboratory diagnosis for its unique properties such as cost-effective, rapid, sensitive and specific and play a role in POCT and rapid diagnosis.This paper introduces the principle and advantages of electrochemical immunosensor.Biological molecules such as tumor marker, myocardial marker, hormone, pathogenic bacteria, viruses, and autoimmune antibody has carried on the induction,based on the domestic and foreign research results.The advantages and disadvantages of electrochemical immunosensor was evaluated, and the research on its application prospect is analyzed.
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Objective To preparegraphene quantum dots (GQDs) and construct a novel biosensor for determination of dopamine (DA) and uric acid (UA).Methods The GQDs was prepared by carbonization method from citric acid as carbon sources.Differential pulse voltammetry was used by the modified electrodes to detect uric acid and dopamine,and the detection performance was evaluate.Collected three experimenters morning urine on July 29,2016.The proposed sensor was used for biological samples detection.Results The size of GQDs were between 3 to 5 nm,which was observed by transmission electron microscopy (TEM).The proposed sensor had good linear correlation of 50～600 μmol/L UA (r2 =0.996 6) and 2～ 240 μmol/L DA (r2 =0.992 5).In uric acid in urine samples' detection (n=3),RSD value was less than 3％.The standard addition recovery rates of UA and DA were in 95.7％～101.4％ and 96％～102％ respectively.Conclusion The biosensor based on GQDs for determination DA and UA had been constructed successfully.
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Objective To evaluate the clinical effect of transforaminal decompression posterior interbody fusion internal fixation in the treatment of upper protrusion of lumbar intervertebral disc .Methods 11 patients with upper protrusion of lumbar interverte-bral disc were given transforaminal decompression posterior interbody fusion internal fixation .The operation time and bleeding a-mount were recorded ,and the clinical effects were evaluated .Results The operation time and bleeding amount during operation were (132 .0 ± 37 .0)min and (320 .1 ± 56 .8)mL ,respectively .The lumbar function scoring of Japanese Orthopedic Association (JOA) after operation was (22 .9 ± 2 .3) points ,which was significantly higher than (11 .2 ± 3 .1) points before operation ;The postoperative recovery excellent rate was 90 .9% .All of the patients successfully finished posterior interbody fusion .Conclusion Transforaminal decompression posterior interbody fusion internal fixation in the treatment of upper protrusion of lumbar interverte-bral disc has the advantages of sufficient exposure ,safe operation and significant therapeutic effects .