Antibiotic resisitanc and PFGE genotyping of Klebsiella pneumoniae from neonatal ward infections / 实用医学杂志

Objective To collecct clinical isolates of Klebsiella pneumonia ,detect the sensitivity of antibiotics,determine the characterization of ESBLs-producing strains,type the isolates by PFGE and investingate the clinical data for analysis. Methods ESBLs-producing strains were tested by inhibitor potentiated disk diffusion rcommended by CLSI2015. The susceptibility to 26 different kinds of antibiotics were testd by CLSI2015 disk diffusion method. The typing of the gene of strains by PFGE was analyzed. Results Sixty ESBLs strains produced in 115 strains of Klebsiellapneumoniae ,which was 100% sensitive to imipenem and meropenem in antimicrobial susceptibility ,and were basically resistant to Amoxacillin ,ampicillin ,piperacillin and the first to the fourth generation of cephalosporins. The 60 ESBLs-producing Klebsiellapneumoniae gene was divided into 14 types (A ~ N)by PFGE,of which type A was the epidemic strain(35%,21/60). Conclusion The incidence rate of ESBLs-producing strains is high in respiratory ward in severe resistant multiantibioticsThe epidemic caused by homologusKlebsiella pneumonia can happen. PFGE is a stable and reliable method in searching the source of noso-comial infecton and is suitable for surveillance in the homologus nosocomial infecton.

Antibiotic resisitanc and PFGE genotyping of Klebsiella pneumoniae from neonatal ward infections / 实用医学杂志

Objective To collecct clinical isolates of Klebsiella pneumonia ,detect the sensitivity of antibiotics,determine the characterization of ESBLs-producing strains,type the isolates by PFGE and investingate the clinical data for analysis. Methods ESBLs-producing strains were tested by inhibitor potentiated disk diffusion rcommended by CLSI2015. The susceptibility to 26 different kinds of antibiotics were testd by CLSI2015 disk diffusion method. The typing of the gene of strains by PFGE was analyzed. Results Sixty ESBLs strains produced in 115 strains of Klebsiellapneumoniae ,which was 100% sensitive to imipenem and meropenem in antimicrobial susceptibility ,and were basically resistant to Amoxacillin ,ampicillin ,piperacillin and the first to the fourth generation of cephalosporins. The 60 ESBLs-producing Klebsiellapneumoniae gene was divided into 14 types (A ~ N)by PFGE,of which type A was the epidemic strain(35%,21/60). Conclusion The incidence rate of ESBLs-producing strains is high in respiratory ward in severe resistant multiantibioticsThe epidemic caused by homologusKlebsiella pneumonia can happen. PFGE is a stable and reliable method in searching the source of noso-comial infecton and is suitable for surveillance in the homologus nosocomial infecton.

Surveillance of antibiotic resistance in the bacterial strains isolated from the First Hospital of Qiqihar in 2015 / 中国感染与化疗杂志

Objective To investigate the distribution and antibiotic resistance profile of clinical isolates in the First Hospital of Qiqihar during 2015.Methods Antimicrobial susceptibility test was carried out according to a unified protocol using automated system from January 1,2015 to December 31,2015.The results were analyzed with WHONET 5.6 software according to the 2014 breakpoints of Clinical and Laboratory Standards Institute.Results A total of 5 162 clinical isolates were collected,of which 28.1％ (1 450/5 162) were gram-positive cocci and 71.9％ (3 712/5 162) were gram-negative bacilli.About 36.5％ (255/698) ofS.aureus isolates and 81.4％ (180/221) of coagulase negative Staphylococcus isolates were resistant to methicillin.No S.aureus and coagulase negative Staphylococcus isolate were found resistant to vancomycin or linezolid.Enterococcus isolates showed low resistance to vancomycin and linezolid.One strain of E.faecium was found resistant to vancomycin.ESBLs were produced in 39.9％ (298/747) ofE.coli,26.1％ (294/1 127) ofKlebsiella spp.,and 15.6％ (12/77) ofP mirabilis strains.The Enterobacteriaceae strains were less resistant to imipenem,beta-lactam/beta-lactamase inhibitor combination and amikacin.About 36.6％ (163 / 445) of A.baumannii isolates and 1.8％ (13/715) of P.aeruginosa isolates were extensively drug-resistant strains.Conclusions Antibiotic resistance poses a serious threat to clinical practice,to which more attention should be paid.Clinical microbiology lab should make more efforts to provide better support to clinical therapy.

Sensory cueing in the treatment of unilateral spatial neglect / 中华物理医学与康复杂志

Objective To investigate the effects of sensory cueing (SC) on unilateral spatial neglect after stroke.Methods Five stroke survivors with unilateral spatial neglect underwent a tailored sensory cueing treatment (wearing a sensory cueing device 3 hours a day, 5 days a week for 2 weeks) in addition to their conventional rehabilitation.Two weeks before and one day before the treatment, and then one day, two weeks and 4 weeks after the treatment, all five patients were assessed using the Hong Kong edition of the behavioral inattention test (BIT-C).Results No significant changes were identified in the average BIT-C ratings at the two time point before the intervention.However, the average score had increased significantly only one day after the start of the intervention, with further significant improvement at each of the succeeding 2 week intervals.The greatest improvement was in finishing cancellation tasks, and the most severely affected patient showed the greatest improvement.Conclusion Sensory cueing treatment may be useful and feasible in reducing unilateral spatial neglect for stroke survivors.However, randomized and controlled trials with larger samples are needed to further verify its effects.

Treatment of cervical opening wound (report of 43 cases) / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To summarize our experiences on surgical saving of the cervical opening wound.@*METHOD@#Retrospectively analyze 43 cases with cervical opening wound in department of otolaryngology, suizhou central hospital from 1992-2010.@*RESULT@#Forty-one among 43 cases healed with no complications, 1 case died of obstruction of air way and hemorrhagic shock, and 1 case occurred secondary laryngostenosis and resulted in fine prognosis af ter treating with laryngotracheoplasty in higher hospital.@*CONCLUSION@#It is very important to give the appropriate treatment on the injuries of large cervical vessels, nerves, laryngeal cartilages, throat mucosa and complications. It is important to repair the wounds primarily. Correct diagnosis and treatment at first time, and well nursing are crucial for good prognosis.

Expression and significance of SP-A in nasal mucosa of allergic rhinitis and nasal polyp / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#Surfactant protein A (SP-A) is protein that appears to play an important role in mammalian first-line host defense. The objective of this study was to immunolocalize SP-A in human sinonasal tissue.@*METHOD@#Eleven cases of allergic rhinitis, fifteen cases of polyp and seven cases of normal middle turbinate were studied with immunohistochemistry and immunofluorescence method to detect the expression of SP-A.@*RESULT@#The expression of SP-A in allergic rhinitis and polyp were dramatically higher than that in controls (P 0.05). The result demonstrated that SP-A was positively correlated with eosinophils within the basement membrane of epithelium (R = 0.81, 0.55). In the result of immunofluorescence, there was significantly higher expression SP-A in nasal mucosa of allergic rhinitis and nasal polyp than that in control group (P < 0.05).@*CONCLUSION@#SP-A is likely to play key roles in the inflammatory reaction process of allergic rhinitis and polyp. Its secretion in the upper airway indicates that future studies may allow manipulation of this protein and development of novel treatments for sinonasal pathology.

Expression and significance of SP-A in nasal mucosa of allergic rhinitis and nasal polyp / 临床耳鼻咽喉头颈外科杂志

Objective: Surfactant protein A (SP-A) is protein that appears to play an important role in mammalian first-line host defense. The objective of this study was to immunolocalize SP-A in human sinonasal tissue. Method:Eleven cases of allergic rhinitis, fifteen cases of polyp and seven cases of normal middle turbinate were studied with immunohistochemistry and immunofluorescence method to detect the expression of SP-A. Result:The expression of SP-A in allergic rhinitis and polyp were dramatically higher than that in controls(P0.05). The result demonstrated that SP-A was positivly correlated with eosinophils within the basement membrane of epitheli-um(R=0.81,0.55). In the result of immunofluorescence, there was significantly higher expression SP-A in nasal mucosa of allergic rhinitis and nasal polyp than that in control group(P<0.05). Conclusion:SP-A is likely to play key roles in the inflammatory reaction process of allergic rhinitis and polyp. Its secretion in the upper airway indicates that future studies may allow manipulation of this protein and development of novel treatments for sinonasal pathology.

Expression of human protection of telomeres1 (POT1) and telomeric repeat binding factor-2 (TRF2)in laryngneal squamous cell carcinoma / 中国耳鼻咽喉头颈外科

OBJECTIVE To study the significance by analyzing the expression of human protection of telomeres1(POT1)and telomeric repeatbinding factor-2(TRF2)in the tissues of laryngneal squamous cell carcinoma(LSCC)and in polyp of vocal cord tissues. METHODS The expression of POT1 and TRF2 in 20 tumor samples and 19 polyp of vocal cord were determined by the indirect immunofluorescence method. The results were scored and analyzed statistically. RESULTS The positive expression rates of POT1 and TRF2 in tumor samples was 65.00% and 70.00% respectively. There were no positive expression of POT1 and TRF2 in polyp of vocal cord. The positive expression of POT1 was higher in poorly differentiated laryngeal carcinoma than that in well-differentiated and moderately differentiated laryngeal carcinoma(Chi-square test with contingency table, P0.05). CONCLUSION The expression of POT1 and TRF2 in laryngneal squamous cell carcinoma were remarkable, POT1 and TRF2 may play a critical role in tumorigenesis of laryngneal squamous cell carcinoma. There was statistical significant difference for degrees of POT1 expression in different tumor histological grades.