RESUMO
lymphocyte,and especially the small lymphocytes were moreheavily labeled than the larger ones,and the Con A receptor of NK cell exhibitedpolarization along the cell surface.These results suggest that:1.the extent of ConA receptors was related to the functional activities of the ceils,the more active thecell is,the more the Con A receptors will be;2.the receptors of Con A wereprobably related to the cell recognition,for instance,on the NK cell Con A rece-ptors showed polar distribution;3.the number of receptors of Con A was dependenton the cell differentiation,the more mature the cell is,the more the Con Areceptors are.
RESUMO
In order to observe the effects of the supernatant of WEHI-3 cell culture which contains IL-3 (interleukin 3), we have Performed some experiments by adding WEHI-3 supernatant to murine bone marrow cells culture. Cell culture of six days shows that the supernatant of WEHI-3 can promote adherence and proliferation of the stromal cells. There are 1.334?10~6 stromal cells/L in the culture which contained 20% WEHI-3 supernatant. Four kinds of stromal cells can be identified under both light microscope and SEM. Fibroblast-like cells which tend to be spindle-shaped with many filament-like and microvilli-like dendrites on the cell surface; Riticular-like cells which are irregular with some folds on the dendrites surface occasionally; Macrophage-like cells which are round with many phagocytic granules in the cytoplasm, and are characterized by positive ANAE staining, and many lamina-like folds and long filament-like dendrites on the cell sufrace; Fat cells are few in number, the cell body is ovoid with lipid droplet, and show strong cytoplasmic staining for Sudan black B, the cell surface is smooth. At the same time we have also observed the close contact between the hematopoietic cells and the stromal cells. In the culture without WEHI-3 supernatant, we have found few small adhered cells with less cytoplasm and weak enzyme activaties under light microscope, and with atrophied cell body and flat surface under SEM. The cell count of the stromal cells in normal culture is 58.83/mm~2. The results showed that WEHI-3 supernatant can promote the growth and proliferation of the stromal cells of murine bone marrow with some histochemical changes.
RESUMO
The effects of the Biological Suppressor of Cancer(BSG)on human normal hone marrow cells and leukaemic cells were studied.There are some evidences that the BSC possesses a biological activities which apparently kill leukaemic cells and inhibit their DNA synthesis,but it has no such effects on erythroblast.The BSC extracted from ascites of ascitic tumors shows no cancer lines specificity,nor tissue specificity.
RESUMO
In this paper, murine natural killer (NK) cells were studied with cytochemistry and electron-microscopy after treated with interleukin-2 (IL-2) and polysaccharide of astragalus (PA). The results showed that acid phosphatase (AcP), acid naphthylacetate esterase (ANAE) and naphthol-AS-D chloroacetate esterase (NCAE) were positive in NK cells, and the activity of AcP and ANAE was enhanced by IL-2 and PA. Significant changes of NK cells in ultrastructure took place after preculturing with IL-2.