RESUMO
Objective:To optimize the alcohol precipitation technology of Dingtongning granule and investigate the rationality of al-cohol precipitation technology by pharmacodynamics. Methods:With the yield of extract and the transfer rates of paeoniflorin and feru-lic acid as the comprehensive evaluation indices,L9(34) orthogonal test was used to optimize the alcohol precipitation technology by taking the concentration of alcohol precipitation,relative density of extract and alcohol precipitation time as the influencing factors,and the efficacy of water extract before and after the alcohol precipitation was compared through the biochemical indices of mice. Results:The optimum alcohol precipitation process was as follows:the extracting liquid was concentrated to the relative density of 1.10 g·ml-1 (25 ℃),and then 90% ethanol was added till the concentration of ethanol up to 70%,and last,the extract was stored in cold for 18 h. The efficacy had no obvious changes before and after the alcohol precipitation. Conclusion:The optimal alcohol precipitation tech-nology is stable and feasible,which can maintain the efficacy and shows good purification. The optimal alcohol precipitation technology provides experimental basis for the scientific production of Dingtongning granule.