RESUMO
Objective:To observe the protective effect of Zhicao Tea Mixture on Müller cells and the expression of inflammatory factors in mice with diabetic retinopathy.Methods:Seventy-five C57BL/6J mice were randomly divided into the normal control group, diabetes mellitus (DM) group, low concentrations group, medium concentrations group and high concentrations group, with 16 mice in each group. The diabetes model of mice in all groups except the normal control group were established by intraperitoneal injection of STZ (60 mg/kg). Four weeks after the successful modeling, the Zhicao Tea Mixture with low (30 ml/kg), medium (60 ml/kg) and high concentrations (120 ml/kg) were respectively administered by gavage. Weight and blood glucose of mice in each group were measured every two weeks. After 8 weeks, Western blot method was used to detect the mice retina Müller cells activation marker gelatinous fibrous acidic protein (GFAP). Immunofluorescence was performed to detect the expression GFAP and glutamine synthetase (GS). Real-time quantitative PCR (RT- qPCR) and ELISA were used to determine the mRNA and protein expression levels of mouse retinal VEGF, TNF-α, IL-1β and IL-6 respectively.Results:The weight of mice in the DM group was lower than that of the normal control group, and the blood glucose was increased. Zhicao Tea Mixture had no effect on the weight of DM mice, but had a significant hypoglycemic effect. The GFAP expression ( t=38.318, P<0.001) in the retina of mice in the DM group was increased and GS expression ( t=29.737, P<0.001) was decreased compared with the control group. The GFAP expression ( t=13.677, 19.387, 16.305; P<0.05) in the retina of mice in the low, medium and high concentrations group were decreased and GS expression ( t=5.170, 19.399, 6.705; P<0.05) were increased compared with the DM group. The expressions of retinal inflammatory factors VEGF, TNF-α, IL-1β and IL-6 in DM group all increased, while the expressions of the above-mentioned inflammatory factors in the retina of mice decreased in the low, medium and high concentrations group. Conclusion:Zhicao Tea Mixture can decrease the blood glucose of DM mice and reduces the diabetic retinal inflammatory response.
RESUMO
Objective:To study the inhibitory effect of bone marrow mesenchymal stem cells (MSC) on the proliferation of allogeneic T lymphocytes in patients with acute leukemia and its mechanism. Methods: 30 patients with acute myeloid leukemia ( AML) ,30 patients with acute lymphoblastic leukemia ( ALL) ,and 30 healthy subjects ( healthy group) were selected. MSC cells were isolated and cultured in three groups. The expression level of MSC cells in three groups were determined by MSC assay. The inhibitory effect of MSC on the proliferation of T cells was detected by Transwell assay. Results: AML group supernatant TGF-β1,HGF levels were significantly lower than the ALL group and the healthy group (P0. 05). AML,ALL,MSC cells secrete cytokines healthy group of T lymphocyte proliferation still had a significant inhibitory effect, inhibition of differences in the case of contact and not in direct contact co-culture of T lymphocyte proliferation was not statistically sig-nificant (P> 0. 05); after joining the MSC,T cell proliferation in the three groups was significantly inhibited upon addition of anti-TGF-β1 antibody,anti-HGF antibody after T cell proliferation effective than a simple reversal join MSC (P<0. 05). Conclusion:MSC for acute leukemia patients inhibit allogeneic T lymphocyte proliferation,functioning principle may be related to the secretion of cyto-kines.